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Abstract: A water-soluble polysaccharide obtained from Acanthopanax senticosus leaves (ASL), was fractionated by DEAE–Sepharose fast-flow column chromatography, and purified by Sephadex G-75 gel-permeation column chromatography. The characteristics of ASP-2-1 were determined by chemical analysis, high-performance capillary electrophoresis (HPCE), high-performance gel-permeation chromatography (HPGPC). The results show that ASP-2-1 contained 89.47% carbohydrate, 7.45% uronic acid, 2.16% protein and seven kinds of monosaccharides including rhamnose, xylose, glucose, mannose, arabinose, galactose and glucuronic acid in a molar ratio of 7.45:18.63:25.15:0.93:8.35:2.79:5.69, with an average molecular weight of about 14,573Da. Furthermore, the immunobiological and antioxidant activities, in vitro, of ASP-2-1 were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and ferric-reducing antioxidant power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH ), superoxide radical and hydroxyl radical ( OH) free radical-scavenging assay, respectively. The results showed that ASP-2-1 exhibited significantly higher immunomodulatory activities against the lymphocyte proliferation in vitro, pronounced reductive power (FRAP value: 785.1μM at 0.2mg/ml), strong hydroxyl radical (89.56% at 1mg/ml) scavenging activity, moderate superoxide radicals (65.32% at 1mg/ml) and DPPH radicals (68.9% at 1mg/ml) scavenging activities. ASP-2-1 should be explored as a novel and potential natural antioxidant and immunostimulating agent for use in functional foods or medicine. [Copyright &y& Elsevier]

Abstract: Four water-soluble polysaccharides fractions (ZSP1b, ZSP2, ZSP3c and ZSP4b) were isolated and purified from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao by DEAE-SepharoseCL-6B and SepharoseCL-6B column chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). Antioxidant activities of crude polysaccharide from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao (CZSP) and its purified fractions were investigated including superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging activity and reducing power. Antioxidant results showed that ZSP3c and ZSP4b containing more uronic acid had the stronger free radical scavenging activities than ZSP1b containing no uronic acid. Available data obtained with in vitro models suggested that uronic acid was an effective indicator of antioxidant activity of the samples. [ABSTRACT FROM AUTHOR]

Abstract: Water-soluble salvianolic acid A (Sal A) and salvianolic acid B (Sal B) were successfully isolated and purified from the crude extract of Salvia miltiorrhiza by high-speed counter-current chromatography (HSCCC). The solvent system was n-hexane–ethyl acetate–methanol–water (3:6:6:10, v/v/v/v). 4.27mg of Sal A and 32.09mg of Sal B were obtained from 260mg of the crude sample. The purities of Sal A and Sal B were 96.67% and 97.43%, respectively. Their structures were identified by 1H NMR and 13C NMR. Antioxidant activities of Sal A and Sal B were also evaluated and compared by the methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS +) radical cation decolourisation assay. Both Sal A and Sal B showed high radical scavenging activities with their EC50 values being 1.43±0.09 and 1.81±0.01μg/ml in DPPH radical method. The ABTS results showed that Sal A and Sal B exhibited high total antioxidant activities, their EC50 values were 1.35±0.00 and 1.43±0.01μg/ml, respectively. [Copyright &y& Elsevier]