1. The protective effect of 17β-estradiol against hydrogen peroxide-induced apoptosis on mesenchymal stem cell
- Author
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Chen, Hai-Ying, Zhang, Xian, Chen, Shuang-Feng, Zhang, Ying-Xin, Liu, Yi-Hua, Ma, Long-Le, and Wang, Le-Xin
- Subjects
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ESTRADIOL , *HYDROGEN peroxide , *DRUG efficacy , *MESENCHYMAL stem cells , *DRUG design , *APOPTOSIS , *OXIDATIVE stress - Abstract
Abstract: This study was designed to investigate the function of 17β-estradiol (17β-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2). BMSCs were treated with 17β-E2 for 24h and then treated with 100μM H2O2 for 1h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H2O2 compared to control group. But pretreatment with 17β-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17β-E2 exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17β-E2 can reduce the donor stem cells damage. [Copyright &y& Elsevier]
- Published
- 2012
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