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Abstract Stricter effluent limits, water shortage conditions, land availability requires today even more the needs of advanced wastewater treatments. Attractive solutions come from membrane bioreactors (MBR), Integrated Fixed Film Activated Sludge (IFAS) or combinations (i.e., IFAS-MBRs). One crucial aspect for the applicability of this overall new technology, compared to the conventional activated sludge systems, is the lack of knowledge for design and manage (e.g., kinetic constants, optimal operative conditions etc.). In view of the above frame, the aim of the present study was to assess the kinetic and stoichiometric parameters of bacterial species in MBRs by means of respirometric techniques. Plant configurations, operational conditions and wastewater features (domestic/industrial) were analysed. Four different MBR plants were investigated: i) sequencing batch MBR subjected to a gradual salinity increase; ii) pre-denitrification MBR treating saline wastewater contaminated by hydrocarbons; iii) University of Cape Town (UCT) MBR treating domestic wastewater subjected to a carbon-to-nitrogen (C/N) ratio variation; iiii) UCT- IFAS -MBR treating domestic wastewater. Results show a significant influence on biomass respiratory activity from both plant configurations and operational conditions. The salinity increase severely affected the activity of autotrophic species, while heterotrophic community was mainly influenced by the C/N variation. Moreover, it was observed a specialization in the IFAS-MBR configuration, with the suspended biomass more affine to organic matter, whilst biofilm in the nitrification process. The respirometric analysis confirmed to be an effective tool for the evaluation of the biomass kinetic and stoichiometric parameters. The results of this study can be useful for the application of mathematical models in the design phase and for the monitoring of biomass viability during plant operations. Graphical abstract Image 1 Highlights • The study aim was to provide new insights on biokinetic behaviour of MBRs. • Respirometry was used to assess the kinetic and stoichiometric coefficients. • Heterotrophs highly suffered low C/N ratio with increased membrane fouling. • Salinity highly affected the autotrophic activity increasing the pore blocking. • It was observed a specialization of suspended and attached biomass in the IFAS-MBR. [ABSTRACT FROM AUTHOR]

The present study has focused on the mainstream integration of polyhydroxyalkanoate (PHA) production with industrial wastewater treatment by exploiting three different technologies all operating in sequencing batch reactors (SBR): conventional activated sludge (AS-SBR), membrane bioreactor (AS-MBR) and aerobic granular sludge (AGS). A full aerobic feast/famine strategy was adopted to obtain enrichment of biomass with PHA-storing bacteria. All the systems were operated at different organic loading (OLR) rate equal to 1-2-3 kgCOD/m3∙d in three respective experimental periods. The AS-MBR showed the better and stable carbon removal performance, whereas the effluent quality of the AS-SBR and AGS deteriorated at high OLR. Biomass enrichment with PHA-storing bacteria was successfully obtained in all the systems. The AS-MBR improved the PHA productivity with increasing OLR (max 35% w/w), whereas the AS-SBR reduced the PHA content (max 20% w/w) above an OLR threshold of 2 kgCOD/m3∙d. In contrast, in the AGS the increase of OLR resulted in a significant decrease in PHA productivity (max 14% w/w) and a concomitant increase of extracellular polymers (EPS) production (max 75% w/w). Results demonstrated that organic carbon was mainly driven towards the intracellular storage pathway in the AS-SBR (max yield 51%) and MBR (max yield 61%), whereas additional stressors in AGS (e.g., hydraulic selection pressure, shear forces) induced bacteria to channel the COD into extracellular storage compounds (max yield 50%) necessary to maintain the granule's structure. The results of the present study indicated that full-aerobic feast/famine strategy was more suitable for flocculent sludge-based technologies, although biofilm-like systems could open new scenarios for other biopolymers recovery (e.g., EPS). Moreover, the AS-MBR resulted the most suitable technology for the integration of PHA production in a mainstream industrial wastewater treatment plant, considering the greater process stability and the potential reclamation of the treated wastewater. [Display omitted] • A mainstream process for PHA production was integrated with wastewater treatment. • A comparison between AS, AGS and MBR as the enrichment/treatment stage was assessed. • MBR enabled the highest PHA production potential and effluent water eligible for reuse. • Biomass enrichment under full aerobic in AGS promoted extracellular storage pathways. • Integration of PHA production in the mainstream of a WWTP resulted feasible using MBR. [ABSTRACT FROM AUTHOR]

Abstract: In urban water quality management, several models are connected and integrated for analysing the fate of pollutants from the sources in the urban catchment to the final recipient; classical problems connected with the selection and calibration of parameters are amplified by the complexity of the modelling approach increasing their uncertainty. The present paper aims at studying the influence of reductions in available data on the modelling response uncertainty with respect to the different integrated modelling outputs (both considering quantity and quality variables). At this scope, a parsimonious integrated home-made model has been used allowing for analysing the combinative effect of data availability regarding the different parts of the integrated urban drainage system; the uncertainty analysis approach has been applied to an experimental catchment in Bologna (Italy). The number of available data points has been fictitiously reduced obtaining data sets ranging between 25% and 100% of the actually measured data. For each of the data sets, uncertainty has been evaluated and its propagation from the upstream sub-model to the downstream ones has been assessed. The present study demonstrates that model calibration and modelling efficiency assessment may induce the operator to be excessively confident in the model results when available data are scarce. Quite the opposite is indeed true, that limited data availability increases modelling uncertainty. A conclusion of this article is that uncertainty analysis should always be conducted in order to effectively evaluate model reliability. [Copyright &y& Elsevier]

Urban stormwater quality modelling plays a central role in evaluation of the quality of the receiving water body. However, the complexity of the physical processes that must be simulated and the limited amount of data available for calibration may lead to high uncertainty in the model results. This study was conducted to assess modelling uncertainty associated with catchment surface pollution evaluation. Eight models were compared based on the results of a case study in which there was limited data available for calibration. Uncertainty analysis was then conducted using three different methods: the Bayesian Monte Carlo method, the GLUE pseudo-Bayesian method and the GLUE method revised by means of a formal distribution of residuals between the model and measured data (GLUE_f). The uncertainty assessment of the models enabled evaluation of the advantages and limitations of the three methodologies adopted. The models were then tested using the quantity–quality data gathered for the Fossolo catchment in Bologna, Italy. The results revealed that all of the models evaluated here provided good calibration results, even if the model reliability (in terms of related uncertainty) varied, which suggests the adoption of a specific modelling approach with respect to the others. Additionally, a comparison of uncertainty analysis approaches showed that, regarding the models evaluated here, the classical Bayesian method is more effective at discriminating models according to their uncertainty, but the GLUE approach performs similarly when it is based on the same founding assumptions as the Bayesian method. [Copyright &y& Elsevier]

Abstract: In urban drainage, new computational possibilities have supported the development of new integrated approaches aimed at joint water quantity and quality analysis of the whole urban drainage system. Although the benefit of an integrated approach has been widely demonstrated, to date, several aspects prevent its applicability such as scarce availability of field data if compared with model complexity. These aspects sometimes prevent the correct estimation of parameters thus leading to large uncertainty in modelling response. This is a typical parameter identifiability problem that is discussed in the present paper evaluating the effect of identifiability procedures in increasing operator confidence in modelling results. The methodology presented has been applied to a home-made integrated urban drainage model that has been calibrated/validated considering field data collected in the Savena experimental catchment (Bologna, Italy). The results demonstrate the effectiveness of the identifiability analysis in obtaining a tool for urban integrated modelling applications and field data gathering campaigns. [Copyright &y& Elsevier]

The aim of this work was to gain insights about the feasibility of chlorinated solvents removal through biostimulated and bioaugmented biological processes in laboratory-scale permeable reactive barriers (PRBs) under anaerobic and aerobic conditions. The experimental plant consisted of two Plexiglas cylindrical columns filled with silica sand and fed with real groundwater contaminated by chlorinated solvents (mainly 1,2-dichloroethane, 1,2-DCA, at a concentration of 20 mg l−1). Column A simulated a PRB containing poly-β-hydroxybutyrate (PHB) powder as electron donor and worked under anaerobic conditions; in Column B an inlet air flow rate ensured aerobic conditions. Both columns were inoculated with dechlorinating bacterial consortia obtained by enrichment cultures from the same contaminated groundwater. Results from Column A showed that PHB can be fermented and used as a slow-releasing carbon source for sustaining reductive dechlorination, as revealed by acetate production up to 267 mg l−1 and 100% 1,2-DCA removal. The microbial community detected in Column A at the end of the experimental period was mainly enriched in sulfate reducing bacteria that could act as both fermenting and dechlorinating agents. Column B showed a slight lower 1,2-DCA removal efficiency (98%) likely related to the establishment of aerobic (co)metabolic processes. [Display omitted] • 98–100% (an)aerobic 1,2-DCA removal from a real contaminated groundwater in PRB. • PHB used as slow-release acetate source for anaerobic reductive dechlorination, 267 mg l−1. • Establishment of direct or cometabolic oxidative biodegradation in aerobic PRB. • Biodegradation confirmed by metagenomic detection of dehalogenating bacteria. • Feasibility of a bioremediation approach using bioaugmented and biostimulated PRBs. [ABSTRACT FROM AUTHOR]

In this study the occurrence and the behavior of illicit drugs and their metabolites have been investigated for two wastewater treatment plants (WWTPs) (namely, WWTP-1 and WWTP-2) located in Sicily (island of Italy). Samples were analyzed for methamphetamine, cocaine (COC), 3,4-methylenedioxymethamphetamine (MDMA), methadone (METH), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), 3,4-methylenedioxy amphetamine (MDA); 3,4-methylenedioxy ethylamphetamine (MDEA), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) and Benzoylecgonine (BEG). The BEG, COC, MOR and THC-COOH were found at the highest concentration in both WWTPs. The Wastewater-based epidemiology calculation for BEG, COC, cannabinoids and THC-COOH was performed. On average, for both plants, population consumes 1.6 and 23.4 dose 1000 inh −1 day −1 of cocaine and cannabis, respectively. For WWTP-1 negative removals of illicit drugs were observed. For WWTP-2 the following average removal efficiencies were obtained: BEG (77.85%), COC (92.34%), CODEINE (64.75%), MOR (90.16%) and THC-COOH (68.64%). [ABSTRACT FROM AUTHOR]

In the present research, insights about the mechanisms of excess sludge minimization occurring in an oxic-settling-anaerobic (OSA) were provided. The investigation involved two systems operating in parallel. In particular, a conventional activated sludge (CAS) system as control and a system implementing the OSA process both having a pre-denitrification scheme were considered. Five periods (P1–P5) were studied, during which several operating conditions and configurations were tested. Specifically, the hydraulic retention time (HRT) in the anaerobic reactor of the OSA system (P1 8 h, P2–P3 12 h, P4 8 h, P5 12 h) and the return sludge from the anaerobic to the anoxic (scheme A) (P1–P2) or aerobic (scheme B) mainstream reactors (P3–P5) were investigated. The results highlighted that the excess sludge production in the OSA was lower in all the configurations (12–41%). In more detail, the observed yield (Y obs) was reduced from 0.50-0.89 gTSS gCOD−1 (control) to 0.22 -0.34 gTSS gCOD−1 in the OSA process. The highest excess sludge reduction (40%) was achieved when the OSA was operated according to scheme B and HRT of 12 h in the anaerobic reactor (P3). Generally, scheme A enabled the establishment of cell lysis and extracellular polymeric substances (EPS) destructuration, leading to a worsening of process performances when high anaerobic HRT (>8 h) was imposed. In contrast, scheme B enabled the establishment of maintenance metabolism in addition to the uncoupling metabolism, while cell lysis and EPS destruction were minimized. This allowed obtaining higher sludge reduction yield without compromising the effluent quality. [Display omitted] • An OSA process with a novel plant layout enabled excess sludge reduction up to 40%. • Cell lysis and EPS destruction were enhanced by operating under prolonged anaerobiosis. • Cell lysis and EPS hydrolysis impaired effluent quality and sludge settling features. • Maintenance and uncoupling metabolism enabled higher sludge reduction (26–40%). • Nitrifiers were affected by prolonged starvation under not aerobic conditions. [ABSTRACT FROM AUTHOR]

The paper reports the main results of an experimental campaign performed on a membrane bioreactor pilot plant designed to treat synthetic shipboard slops. The experimental campaign was divided into two phases: salinity acclimation up to 20 g NaCl L −1 (Phase I) and hydrocarbon (diesel fuel) dosing (Phase II). The observed results show that the carbon removal was not severely affected by the wastewater features. Conversely, respirometric tests showed that nitrification was strongly affected by the salinity (33% of nitrification efficiency at 20 g NaCl L −1 – Phase I) as a result of the salinity in the autotrophic biomass. Moreover, the sludge viscosity increased during Phase II due to the wastewater composition, leading to an increase in the membrane resistance, and severe degradation of the sludge dewaterability was also observed. Indeed, the capillary suction time increased by a factor of 3 times compared with that of Phase I. [ABSTRACT FROM AUTHOR]

The time required to stabilise mature aerobic granules is rather variable. In addition, cultivation time and the structural characteristics of granules seem to be related to the nature of wastewater influent. Granular sludge has been used for the treatment of several industrial wastewaters, but nothing has been reported about wastewater characterized by the simultaneous presence of hydrocarbons and high chloride concentration. In this work, the authors analysed the granulation process and performance as well as the physical characteristics of aerobic granules in two Granular Sequencing Batch Airlift Reactors (GSBARs), fed with acetate-based synthetic wastewater in reactor 1 (R1) and with a mixture of real and simulated slop (R2). The results obtained in 100 days show that full granulation was achieved in both reactors. The granules in R2 developed more quickly, but they appeared slightly unstable and more susceptible to breaking. Despite high salt concentration, the efficiency of phosphorous and carbon removal was satisfactory. Low nitrification activity was observed in R1, confirming that a longer time is necessary to obtain the acclimation of autotrophic biomass in aerobic granules. In R2 the combined effect of salinity and hydrocarbons caused the inhibition of the autotrophic biomass, with the consequence that nitrification was absent. Hydrocarbons were initially removed by adsorption afterwards by biological degradation with a removal efficiency of over 90%. [ABSTRACT FROM AUTHOR]

The aim of the present study was to compare the oxygen transfer efficiency in a conventional activated sludge and a membrane bioreactor system. The oxygen transfer was evaluated by means of the oxygen transfer coefficient and α-factor calculation, under different total suspended solids concentration, extracellular polymeric substances, sludge apparent viscosity and size of the flocs. The oxygen transfer coefficient and α-factor showed an exponential decreasing trend with total suspended solid, with a stronger oxygen transfer coefficient dependence in the conventional activated sludge compared to the membrane bioreactor. It was noted that the oxygen transfer coefficient in the conventional activated sludge become comparable to that in membrane bioreactor when the total suspended solid concentration in the conventional activated sludge was higher than 5 g L−1. Operating under high carbon to nitrogen ratio, the oxygen transfer coefficient increased in both conventional activated sludge and membrane bioreactor because of sludge deflocculation and it was noticed a weaker dependence of the oxygen transfer coefficient with total suspended solid. The results indicated that the most important parameters on the oxygen transfer efficiency were in order: the total suspended solid concentration, flocs size, sludge apparent viscosity and extracellular polymeric substances content. Based on the influence of the main biomass features affecting the oxygen transfer coefficient and considering the typical operating conditions in both systems, those of membrane bioreactor appeared to be more favorable to oxygen transfer efficiency. The paper provides a useful insight about some peculiarities of oxygen transfer in a conventional activated sludge and a membrane reactor system, highlighting new and useful information in the light of a more sustainable management of these systems. Image 1 • A comparison between the oxygen transfer in CAS and MBR bench plant was carried out. • (k L a) 20 and α-factor showed exponential trends with TSS for both CAS and MBR. • (k L a) 20 values higher in MBR within a TSS range between 4 gTSS L−1 and 6 gTSS L−1. • Under similar conditions features of sludge in MBR more favorable to oxygen transfer. • TSS, EPS and viscosity should be controlled to optimize aeration efficiency. [ABSTRACT FROM AUTHOR]