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Abstract: A water-soluble polysaccharide (FUP-1) was obtained from Fritillaria ussuriensis Maxim through warm water extraction, ethanol precipitation, anion-exchange and gel-permeation chromatography. Structural analysis of this compound revealed that the average molecular weight of FUP-1 was 4.1×104 Da, and the major monosaccharide component was xylose, followed by glucose and galactose, at low levels. In vitro antioxidant assays showed that FUP-1 exhibited strong hydroxyl, superoxide anion and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activities. During in vivo antioxidant testing, the administration of FUP-1 could largely attenuate the increase in d-gal-induced malondialdehyde (MDA) and renew the activities of superoxide dismutase (SOD) in serums as well as glutathione peroxidase (GSH-Px) and the total antioxidant capacity (T-AOC) in the livers of d-gal-treated mice. Overall, the results of the present study indicate that FUP-1 exerts antioxidant activity not only through its own radical-scavenging activity but also by boosting the enzymatic and nonenzymatic antioxidant defense system of the host. [Copyright &y& Elsevier]

Abstract: Four water-soluble polysaccharides fractions (ZSP1b, ZSP2, ZSP3c and ZSP4b) were isolated and purified from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao by DEAE-SepharoseCL-6B and SepharoseCL-6B column chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). Antioxidant activities of crude polysaccharide from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao (CZSP) and its purified fractions were investigated including superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging activity and reducing power. Antioxidant results showed that ZSP3c and ZSP4b containing more uronic acid had the stronger free radical scavenging activities than ZSP1b containing no uronic acid. Available data obtained with in vitro models suggested that uronic acid was an effective indicator of antioxidant activity of the samples. [ABSTRACT FROM AUTHOR]