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1. Prolonged DNA hydrolysis in water: A study on DNA stability.

Author

Fattorini P, Marrubini G, Bonin S, Bertoglio B, Grignani P, Recchia E, Pitacco P, Procopio F, Cantoni C, Pajnič IZ, Sorçaburu-Cigliero S, and Previdere C

Abstract

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.

Published
2018
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