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The enzyme 3-hydroxykynurenine transaminase (HKT) acts as an important enzyme in tryptophan catabolism of disease-carrier insects, e.g. Aedes aegypti and Anopheles gambiae . HKT is a detoxification enzyme that converts 3-hydroxykynurenine (a precursor for reactive nitrogen and oxygen species) into xanthurenic acid (stable and nontoxic compound). We have previously synthesized eleven new oxadiazole derivatives and demonstrated their noncompetitive inhibitory activity towards HKT from A. aegypti (https://doi.org/10.1016/j.bmc.2019.115252). These findings are presented in a research paper accompanying the present technical report on a new assay to overcome the fact that the substrate and product of the HKT-catalyzed reaction exhibit maximum absorption at very near wavelength (370 and 369 nm, respectively). The methods previously described in the literature rely on chromatographic separation prior to absorbance quantification, which limits their use for inhibitor screening. Due to HKT attractive features as a molecular target for larvicidal compounds, we report herein a new, faster and affordable methodology to evaluate the enzymatic activity of recombinant HKT, and therefore allow for the fast screening of potential HKT inhibitors via absorbance spectrophotometer. The advantages of the proposed methodology to previously described ones are:•It is faster and cheaper than HPLC-based assays because it does not require the use of chromatography columns and solvents to separate reaction components;•It uses of 96-well plates, enabling the simultaneous quantification of samples;•It can be applied to all transaminases that have xanthurenic acid as a product., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2020 The Author(s). Published by Elsevier B.V.)

This article contains a dataset related to the research published in "The potential of the pecan nut cake as an ingredient for the food industry" [1]. A three-component simplex-centroid mixture design coupled with response surface methodology (RSM) was applied to generate statistical models and to analyze the dataset. The method was also applied to evaluate the effect of different solvents (ethanol, water, and acetic acid) on the extraction of bioactive compounds of pecan nut cake (PNC) and its antioxidant activity. Furthermore, simultaneous optimization of the solvent mixture was carried out to predict the optimum point with the best combination of solvents to obtain an extract with enhanced phytochemical composition, as well as high in vitro antioxidant activity. The maximization of total phenolic compounds, condensed tannins, and antioxidant activity of the PNC was predicted by the desirability function. A total of 80 interactions were run to provide the best condition for optimization. The combined use of the different solvents enables a higher recovery of the compounds than their isolated use. This dataset may help other researchers on the application of a mixture design to recover phytochemicals from a broad range of co-products such as defatted meals and other nut cakes, which are sometimes discarded as waste by many industries., (© 2020 The Author(s).)