1. Analysis of neurotransmitters in Daphnia magna affected by neuroactive pharmaceuticals using liquid chromatography-high resolution mass spectrometry.
- Author
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Gómez-Canela C, Rovira García X, Martínez-Jerónimo F, Marcé RM, and Barata C
- Subjects
- Animals, Aquatic Organisms drug effects, Aquatic Organisms growth & development, Aquatic Organisms metabolism, Daphnia drug effects, Daphnia metabolism, Duloxetine Hydrochloride analysis, Fluoxetine analysis, Models, Animal, Neurotransmitter Agents metabolism, Sertraline analysis, Venlafaxine Hydrochloride analysis, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity, Chromatography, High Pressure Liquid methods, Daphnia chemistry, Duloxetine Hydrochloride toxicity, Fluoxetine toxicity, Mass Spectrometry methods, Neurotransmitter Agents chemistry, Sertraline toxicity, Venlafaxine Hydrochloride toxicity
- Abstract
Neurotransmission plays an essential role during the central nervous system (CNS) development. During the last years, several studies based on the changes produced in neurotransmitters of aquatic organisms caused by pharmaceuticals have been reported. Daphnia magna, the aquatic ecotoxicological model organism, shares several of the neurotransmitters targeted by antidepressant and other neuro-active drugs with vertebrates. Therefore, a method based on liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) has been applied for the first time to study the levels of 41 neurotransmitters in Daphnia magna under the effect of four different neuro-active pharmaceuticals (sertraline, venlafaxine, duloxetine and fluoxetine). In addition, the performance of LC-HRMS was studied in terms of linearity, sensitivity, intra- and inter-day precision, and overall robustness. The developed analytical method using LC-HRMS is a new tool for neurotoxicology research using the Daphnia magna model. As a result, general differences on the concentrations of those neurotransmitters exposed to the mentioned pharmaceuticals were observed., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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