1. A t-test ranking-based discriminant analysis for classification of free-range and barn-raised broiler chickens by 1 H NMR spectroscopy.
- Author
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de Oliveira ES, Lião LM, Silva AK, Prado CS, Sena MM, and de Almeida Ribeiro Oliveira G
- Subjects
- Animals, Chickens metabolism, Discriminant Analysis, Magnetic Resonance Spectroscopy methods, Anserine analysis, Carnosine analysis
- Abstract
Intensive systems of raising chickens in barns prevail worldwide for financial reasons. In contrast, free-range chickens are raised in better welfare conditions, and preferred by consumers due to their distinctive taste/flavor, having higher market prices. Thus, free-range chickens have been the target of frauds. In this study,
1 H NMR metabolic profiles of breasts of free-range and barn-raised broilers (108 individuals) were compared by two discriminant models, based on t-test ranking and partial least squares (PLS-DA). Both models provided 100 % of correct classification in both training and test sets, being the univariate model based on t-test screening simpler and more robust. Among other differences, barn-raised broilers presented lower carnosine and anserine concentrations, and higher free amino acids contents. Univariate discrimination was based on the ratio of two NMR signals assigned to β-alanine and carnosine + anserine, respectively. As an additional advantage, this profiling method could be adapted to other measurement platforms., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2023
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