1. Ginger extract and zingerone ameliorated trinitrobenzene sulphonic acid-induced colitis in mice via modulation of nuclear factor-κB activity and interleukin-1β signalling pathway.
- Author
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Hsiang CY, Lo HY, Huang HC, Li CC, Wu SL, and Ho TY
- Subjects
- Animals, Colitis chemically induced, Colitis genetics, Female, Guaiacol administration & dosage, Humans, Interleukin-1beta genetics, Mice, Mice, Inbred BALB C, NF-kappa B genetics, Signal Transduction drug effects, Trinitrobenzenesulfonic Acid adverse effects, Colitis drug therapy, Colitis metabolism, Zingiber officinale chemistry, Guaiacol analogs & derivatives, Interleukin-1beta metabolism, NF-kappa B metabolism, Plant Extracts administration & dosage
- Abstract
Ginger is a commonly used spice with anti-inflammatory potential. Colitis is the common pathological lesion of inflammatory bowel diseases. In this study, we investigated the therapeutic effects of ginger and its component zingerone in mice with 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Ginger and zingerone ameliorated TNBS-induced colonic injury in a dose-dependent manner. Pathway analysis of ginger- and zingerone-regulated gene expression profiles showed that ginger and zingerone significantly regulated cytokine-related pathways. Network analysis showed that nuclear factor-κB (NF-κB) and interleukin-1β (IL-1β) were key molecules involved in the expression of ginger- and zingerone-affected genes. Ex vivo imaging and immunohistochemical staining further verified that ginger and zingerone suppressed TNBS-induced NF-κB activation and IL-1β protein level in the colon. In conclusion, ginger improved TNBS-induced colitis via modulation of NF-κB activity and IL-1β signalling pathway. Moreover, zingerone might be the active component of ginger responsible for the amelioration of colitis induced by TNBS., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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