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Higher nitrite accumulation, which is challenging to achieve reliably, is always sought to obtain better nitrogen removal performance in traditional partial nitritation-anammox (PN/A) process. This study developed a modified PN/A process by introducing nitrite-oxidizing bacteria and endogenous metabolism. Advanced nitrogen removal performance of 95.5 % was achieved at a low C/N ratio of 2.7 under nitrite accumulation ratio (NAR) fluctuations. Higher nitrate accumulation at lower NAR (70 ∼ 40 %) resulted in superior anammox contribution (60 ∼ 75 %) and nitrogen removal performance (93 ∼ 98 %). This was attributed to the higher nitrogen removal efficiency of the post-anoxic endogenous partial denitrification coupling anammox process, although the PN/A process occurring first possessed a faster anammox rate of 2.0 mg NH 4 + -N /(g VSS⋅h). The introduction of nitrate allowed more nitrite flow to anammox, promoting a high enrichment of anammox bacteria (Ca. Brocadia, 0.3 % to 2.8 %). This study provides new insights into the practical application of the PN/A process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A novel two-stage partial nitrification-anammox (PN-A) process was developed, achieving nitrogen removal from low carbon/nitrogen ratio municipal sewage under two typical operational modes and seasonal ambient temperatures. When complete nitritation-anammox was performed at temperatures greater than 19.4 °C, the effluent concentration of total inorganic nitrogen (TIN) was 4.1 mg/L, corresponding to a nitrogen removal efficiency (NRE) of 94.3 %. In contrast, when partial nitritation-anammox was performed at temperatures below 19.4 °C, the effluent TIN was 12.3 mg/L, corresponding to a NRE of 83.6 %. The relative abundance of Nitrosomonas and Nitrosomonadaceae increased from 0.02 % to 0.28 %, while Ca. Brocadia decreased from 1.85 % to 1.30 %, with the contribution of anammox to nitrogen removal being highest under low temperatures (19.4℃ to 13.8℃), at 59.0 %. This novel two-stage PN-A process provides a new approach for the stable operation of wastewater treatment plants (WWTPs) under low ambient temperatures., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Stable nitritation is the major challenge for short-cut nitrogen removal from municipal wastewater. This paper demonstrated a rapid achievement of partial nitrification (PN) in an enhanced biological phosphorus removal (EBPR) reactor treating domestic wastewater. Polyphosphate accumulating organisms (PAOs) were enriched operated at a short aerobic HRT (2.0 h) and SRT (10 d), with satisfactory phosphorus removal efficiency (95.9%). Both of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were elutriated simultaneously. Interestingly, AOB recovered much faster than NOB by a subsequent extension of aerobic HRT and SRT, resulting in a rapid development of PN within 15 days. Ammonia oxidation rates of AOB significantly increased by 44.2%, facilitating a high nitrite accumulation rate (NAR) of 95.8%. Genus Tetrasphaera, Halomonas, Paracoccus and Candidatus_Accumulibacter belonging to PAOs accounted for 4.6%. The proliferation of heterotrophs, typically as PAOs, maximized the microbial competition against NOB by favoring AOB activity and synergy with functional bacteria., (Copyright © 2021 Elsevier Ltd. All rights reserved.)