1. A rapid quantitative on-site coronavirus disease 19 serological test.
- Author
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Lee JH, Bae PK, Kim H, Song YJ, Yi SY, Kwon J, Seo JS, Lee JM, Jo HS, Park SM, Park HS, Shin KS, Chung S, and Shin YB
- Subjects
- Antibodies, Viral, Humans, Immunoassay, Immunoglobulin G, Immunoglobulin M, SARS-CoV-2, Sensitivity and Specificity, Serologic Tests, Biosensing Techniques, COVID-19
- Abstract
On-site severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) serological assays allow for timely in-field decisions to be made regarding patient status, also enabling population-wide screening to assist in controlling the coronavirus disease 2019 (COVID-19) pandemic. Here we propose a rapid microfluidic serological assay with two unique functions of nanointerstice filling and digitized flow control, which enable the fast/robust filling of the sample fluid as well as precise regulation of duration and volume of immune reaction. Developed microfluidic assay showed enhanced limit of detection, and 91.67% sensitivity and 100% specificity (n = 152) for clinical samples of SARS CoV-2 patients. The assay enables daily monitoring of IgM/IgG titers and patterns, which could be crucial parameters for convalescence from COVID-19 and provide important insight into how the immune system responds to SARS CoV-2. The developed on-site microfluidic assay presented the mean time for IgM and IgG seroconversions, indicating that these titers plateaued days after seroconversion. The mean duration from day 0 to PCR negativity was 19.4 days (median 20 d, IQR 16-21 d), with higher IgM/IgG titres being observed when PCR positive turns into negative. Simple monitoring of these titres promotes rapid on-site detection and comprehensive understanding of the immune response of COVID-19 patients., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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