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Mosquitoes, as temperature-sensitive ectothermic vectors, exhibit temperature-dependence. This study investigates Culex pipiens pallens (Cx. pallens) responses to abrupt temperature increases and their implications on mosquito physiology. First instar larvae (24hr post hatching) and newly enclosed adults (24hr post emergence) were separately exposed to heat shock regimes of 33 °C, 37 °C, and 42 °C for 3 days alongside a control temperature of 27 °C. Results showed that mortality was triggered at 42 °C within a day. Adult male mosquitoes were less tolerant to all temperatures than larvae and adult females (p < 0.05). Exposing larvae to constant temperatures for 3 days significantly decreased larvae's development time, growth rate and adult emergence (p < 0.05). Reproductive fitness was significantly reduced (p < 0.05) in males emerging from larvae exposed to 37 °C. Life table parameters showed significant increased mortality rate, kill power and decreased life expectancy at the embryonic stage (p < 0.05). Furthermore, heatwaves deactivated the Transient receptor protein ankyrin 1 at 37 °C (p < 0.05) in larvae but not adults. Calmodium, Heat shock protein 90, and small heat shock protein expression were significantly decreased in larvae at 37 °C (p < 0.05) as compared to larvae raised at 33 °C and 27 °C. In conclusion, we classified the heat waves into three categories: adaptable (33 °C), critical (37 °C), and fatal (42 °C). Prolonged exposure of Culex pallens larvae to extreme heat affects the male reproductive output. These findings may serve as an important reference for forecasting vector and pest dynamics and used to tailor mosquito prevention and control measures., Competing Interests: Declaration of competing interest None of the authors have a conflict of interest to disclose, (Copyright © 2024. Published by Elsevier B.V.)

Microplastics (MPs) are frequently detected in the wastewater. Herein, the short-term and long-term effects of polyethylene terephthalate (PET) MPs on anammox granular sludge were investigated and the potential response mechanisms were analyzed. Results showed that although short-term exposure of anammox granular sludge to PET-MPs induced a stress response, the nitrogen removal performance was not significantly affected. By contrast, long-term exposure to PET-MPs inhibited nitrogen removal performance with increased exposure time and PET-MP concentration. The total nitrogen removal efficiency (TNRE) decreased by 28.7 % when sludge was exposed to 200 mg/L of PET-MPs. However, the anammox activity recovered with prolonged operation time, and approximately 87 % of the initial TNRE was recovered after three months. Microbial community evolution and metabolic exchange variations were the potential response mechanisms of anammox granular sludge to PET-MP exposure, with PET-MP exposure decreasing the anammox bacteria growth rate and relative symbiotic bacterial abundance in the anammox consortia and hindering cross-feeding pathways. The findings of this study provide novel insight into anammox behavior when treating wastewater containing PET-MPs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Enestroburin is the first strobilurin fungicide developed by China and has been widely used to control fungal disease for 15 years. Investigation of its photolytic behaviour is essential for the comprehensive evaluation of its ecological risk. The effects of solution pH, humic acid (HA) and Fe(III) ions on photolysis were studied. The direct photolysis rates of enestroburin in the acidic solution (pH = 4) was faster than that in the basic (pH = 7) or neutral condition (pH = 9). HA and Fe 3+ ions inhibited photolysis by the light screening effect. The photolysis of enestroburin was very fast due to the generation of photo-isomers. Seven isomeric products of enestroburin were observed using SFC-MS/MS, and the reaction mechanism for photo-induced isomers was proposed. The reaction occurred on three double bonds, including tautomerism of enol ether and oxonium and the triplet energy transfer of the CC and CN double bond. 12 transformation products (TPs) were identified by screening suspect compounds and non-target compounds, and one product (M-381) was synthesized for confirmation and quantification. A probable transformation mechanism was suggested based on the identified TPs and DFT calculations. The main transformation reactions included hydration, hydrolysis, oxidation, reduction and decarboxylation. Finally, the toxicities of the identified TPs and parent compound to aquatic organisms were predicted using ECOSAR software, and the toxicities of enestroburin and M-381 to daphnia magna were tested in the laboratory. The toxicity classification proposed by ECOSAR is reliable to a certain extent. Enestroburin and 2 TPs (M-313 and M-327) were classified as "very toxic", which may pose a potential threat to aquatic ecosystems., Competing Interests: Declaration of competing interest All authors have approved this version of the article and no conflict of interest exists in the submission of this manuscript. This paper is our original unpublished work and neither the entire paper nor any part of its content has been published or has been accepted elsewhere., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Pyrisoxazole, an isoxazoline-class fungicide, has been registered and used for approximately 19 years. However, its environmental transformation products (TPs) and corresponding ecotoxicological effects remain ambiguous. In this study, the photolysis, hydrolysis, and soil transformation behavior of pyrisoxazole were systematically investigated by indoor simulation experiments and analyzed by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS) and UNIFI software. Transformation products in different environemnts were effectively identfied by a proposed workflow, which organically combined suspect and non-target screening strategies. In total, 17 TPs were screened out. Eight TPs were confirmed using the corresponding reference standards. Structures of another 9 compounds were tentatively proposed based on diagnostic evidence. Among them, 14 products were reported for the first time. The transformation pathways of pyrisoxazole in soil and water were proposed. Pathway analysis demonstrated that the different pH of aqueous solutions had little effect on the pathways, while the influence of different soil types and oxygen conditions was evident. Finally, the toxicity of the proposed TPs to fish and daphnids was predicted using ECOSAR software. These proposed TPs in soil and water, transformation pathways, and predicted ecotoxicity information could provide systematic insight into the fate and environmental risks of pyrisoxazole., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Background: The aim of this study was to present a modified pancreatojejunostomy technique for laparoscopic pancreaticoduodenectomy (LPD) and to evaluate its safety and reliability., Methods: Clinical data from 67 patients who underwent LPD at a single center, from September 2016 to December 2017 were retrospectively collected and analysed. Of these patients, 31 cases were subjected to modified pancreatojejunostomy (modified group), and 36 cases received duct-to-mucosa pancreatojejunostomy (control group) for LPD. We compared and analysed the operative outcomes and postoperative complications between the patients in the two groups., Results: All LPDs were successfully completed. The mean operation time for pancreatojejunostomy in the modified group was obviously lower than that of the control group (30.9 ± 6.6 min vs 45.3 ± 6.1 min, P < 0.01), and the total operative time was also shorter (321.8 ± 63.6 min vs 362.2 ± 59.6 min, P < 0.05) in the modified group. The overall incidence of postoperative complications was similar (29.0% vs 30.6% P = 0.724). Clinically relevant grade B/C POPF occurred in 2 patients (6.5%) in the modified group and 3 patients (8.3%) in the control group (P = 0.947); All cases were cured using conservative treatment., Conclusions: Our modified pancreatojejunostomy technique is safe, effective and easy to manipulate and learn following LPD., (Copyright © 2019 International Hepato-Pancreato-Biliary Association Inc. Published by Elsevier Ltd. All rights reserved.)