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This study aims to develop a stable and efficient magnetic nanocomposite hydrogel (MNCH) for selective removal of methylene blue (MB) and crystal violet (CV). MNCHs with different Fe 3 O 4 contents (0-9 wt%) were synthesized following graft co-polymerization method using sodium alginate, acrylamide, itaconic acid, ammonium persulfate and N,N-methylene bisacrylamide. Among them, MNCH 5 , with 5 wt% Fe 3 O 4 , showed highest removal efficiency (>95 %). Optimal dye removal occurred at pH 10, with 40 min for CV and 60 min for MB using 30 mg dose. MNCH was characterized using various techniques, with X-ray diffraction (XRD) revealing crystallite size of 30.5 nm, and Brunauer-Emmett-Teller (BET) indicating surface area of 59.80 m 2 g -1 . Adsorption kinetics followed fractal pseudo-first-order and fractal Vermeulen diffusion models, reflecting MNCH's heterogeneous nature as suggested by fractal exponent (h) ranging 0.38-0.44, significantly deviating from zero. Langmuir-Freundlich isotherm accurately described the process, demonstrating MNCH's superior affinity for MB (4216.69 mg g -1 ) over CV (3730.17 mg g -1 ). Thermodynamics of MB adsorption was exothermic as suggested by negative ΔH value, while CV adsorption was endothermic. Density functional theory confirmed stronger interaction between MNCH and MB (E ads  = -49.29 kcal mol -1 ) compared to CV (E ads  = -41.30 kcal mol -1 ). These findings underscore MNCH's excellent adsorption capacity, making it promising for removing dyes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Previously, we identified that AP-1 transcription factor FOSL1 is required to maintain cancer stem cells (CSCs) in HNSCC, and an AP-1 inhibitor, T-5224, can eliminate HNSCC CSCs. However, its potency is relatively low, and furthermore, whether T-5224 eradicates CSCs through targeting FOSL1 and whether FOSL1 serves as an effective target for eliminating CSCs in HNSCC, require further validation. We first found that T-5224 can bind to FOSL1 directly. As a proof-of-principle, several cereblon (CRBN)-recruiting PROTACs were designed and synthesized using T-5224 as a warhead for more effective of targeting FOSL1. The top compound can potently degrade FOSL1 in HNSCC, thereby effectively eliminating CSCs to suppress HNSCC tumorigenesis, with around 30 to 100-fold improved potency over T-5224. In summary, our study further validates FOSL1 as an effective target for eliminating CSCs in HNSCC and suggests that PROTACs may provide a unique molecular tool for the development of novel molecules for targeting FOSL1., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)