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Hesperidin exhibits promising potential as a feed additive for augmenting gastric acid secretion in animals. Gastrointestinal function is essential for animal growth and the efficient digestion of dietary nutrients, with gastric acid secretion serving as one of its critical components. The secretion of gastric acid, together with other digestive fluids and substances, significantly influences the digestion and absorption of animal feed, which in turn affects growth performance. However, there is limited research regarding the application of hesperidin as a feed additive to enhance gastric acid secretion. The present study aims to evaluate the efficacy of hesperidin as a feed additive in enhancing gastric acid secretion and to elucidate its underlying mechanisms. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into four groups as follows: the control group receiving only the basal diet, and the other three groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of five replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 days. The growth performance was evaluated by monitoring feed intake and body weight throughout the trial. A four-day nutrient utilization trial was conducted prior to the conclusion of the feeding experiment. Adoption of the total collection method, the collected droppings were weighed and dried at 65 °C. Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, proventriculus, gizzard chyme, and jugular blood samples, The proventriculus and gizzard weight were weighed and the pH of gizzard chyme was measured at the same time. The collected jugular venous blood was used to assess gastrin levels, whereas chicken gizzard chyme was utilized for the analysis of lactate, hydrochloric acid, and pepsin activity. Proventriculus and gizzard tissues were used to evaluate pepsinogen levels, perform hematoxylin-eosin (H&E) staining, conduct enzyme-linked immunosorbent assays (ELISAs) for key proton pump components, and assess proton pump activity. The results demonstrated that, in comparison to the control group, both the 100mg/Kg and 150 mg/Kg groups exhibited a significant increase in final body weight (FBW) and average daily gain (ADG) (P < 0.05). Additionally, the feed to gain ratio (F/G) was significantly reduced in the 150mg/Kg group (P < 0.05). The results of the nutrient utilization trial indicate that all treatment groups had significantly higher levels of dry matter (DM) and ether extract (EE) compared to the control group (P < 0.05). Furthermore, crude protein (CP) and gross energy (ME) were significantly higher in the 100mg/Kg and 150mg/Kg groups than in both the control group and the 50mg/Kg group (P < 0.05). The inclusion of hesperidin in broiler diets leads to significant improvements in stomach development and lactic acid content, while pH and hydrochloric acid content exhibit opposite trends (P < 0.05). Supplementation of broiler diets with hesperidin at doses of 100 mg/Kg and 150 mg/Kg significantly up-regulates pepsin activity and pepsinogen levels (P < 0.05). Incorporation of hesperidin into the broilers' diet significantly enhances parietal cell numbers (P < 0.05). Dietary supplementation of hesperidin in broilers effectively up-regulates key signaling pathways and intracellular signal substances involved in proton pump activation (P < 0.05). The proton pump activity also exhibited a significant increase compared to the control group of 100mg/Kg and 150mg/Kg (P < 0.05) in our findings. In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, and it can improve the growth performance of broilers by increasing gastric acid secretion and promoting nutrient utilization through activation of proton pump. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy.

ABSTRACT: Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays a pivotal role in animal growth and the digestibility of dietary nutrients, with gastrointestinal motor function serving as a crucial component. However, limited research has been conducted on the application of hesperidin as a feed additive to promote gastrointestinal motility. The present study aims to assess the efficacy of Hesperidin as a feed additive in promoting gastrointestinal motility and elucidating its underlying mechanism. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into 4 groups as follows: the control group receiving only the basal diet, and the other 3 groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of 5 replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 d. At 21 d of age, a 5% w/v Evans Blue solution in distilled water was utilized to measure intestinal transit rates (ITR). Gastric emptying (GE) was evaluated by administering a phenol red solution at a concentration of 0.05% w/v (1 mL/broiler). Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, hypothalamus, duodenum, and jugular blood samples. Jugular blood samples were collected for brain-gut peptide content analysis, while gizzard, hypothalamus, and duodenum samples were used for immunohistochemical analysis. Real-time qPCR was performed on gizzard samples. The results demonstrated a significant improvement in the GE and ITR of broilers in all treatment groups compared to the control group (P < 0.05), particularly in the 100mg/Kg and 150mg/Kg hesperidin group. Incorporation of hesperidin into the broilers' diet significantly enhances serum levels of ghrelin, encompassing serotonin (5-HT), motilin (MTL), cholecystokinin (CCK), and Stem Cell Factor (SCF) as well as substance P (SP) in the gizzard and duodenal tissues while reducing vasoactive intestinal peptide (VIP) levels (P < 0.05). The group administered a dosage of 150mg/Kg exhibited the most pronounced effect.Immunohistochemistry analysis revealed that hesperidin supplementation up-regulated SP protein content and down-regulated VIP protein content in the hypothalamus, gizzard, and duodenum of broilers (P < 0.05), with the most pronounced effect illustrated in the 150mg/Kg hesperidin group. Furthermore, addition of hesperidin to broiler feed resulted in a significant up-regulation of protein expression and gene expression related to SCF and The protein expression of Receptor tyrosine kinase (C-Kit) was significantly upregulated in the 150mg/Kg group, while the gene expression of C-Kit was significantly upregulated in the 50 mg/Kg group (P < 0.05). In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, as its dietary supplementation of hesperidin improves gastrointestinal motility through modulation of both “gut-brain axis” signaling pathways and “SCF/C-Kit signaling pathways” within broiler chicken's digestive system. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy.

The dynamic thermal cycle of the directed energy deposition process inevitably leads to considerable residual stress within the as-deposited NiTi components. The high residual stresses in the as-deposited NiTi components were mostly removed by stress-relief annealing at 200 °C for 3 h without detectable change of its chemical composition and microstructure. The incremental hole drilling method was used to measure the residual stresses in samples of both the as-deposited and stress-relief annealed variants. Meanwhile, the changes in residual martensite and dislocation density during cycling were examined by quasi-in-situ EBSD observations. The results indicate that the stability of the cyclic process is jointly influenced by the residual stress and dislocation. When the residual stress was 1.6 %, the superelasticity of the specimens increased from 69.81 % to 95.72 %; After releasing the residual stress at a strain of 2.5 %, the superelasticity of the specimens increased from 63.09 % to 73.70 %. This study provides useful guidance for improving the superelasticity of NiTi alloys fabricated by directed energy deposition.