1. Characterization of the bioactive compounds with efficacy against gout in Guizhi Shaoyao Zhimu Decoction by UHPLC-Q-Orbitrap HRMS combined with network pharmacological analysis
- Author
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Yong-Xiang Gao, Tingna Li, Yijia Zeng, Yuanyuan Ren, Xiaorui Zhang, Tan Jin, Hai-Rong Zeng, and Qinwan Huang
- Subjects
General Chemical Engineering ,Active components ,02 engineering and technology ,010402 general chemistry ,Orbitrap ,Mass spectrometry ,01 natural sciences ,law.invention ,law ,Guizhi-shaoyao-zhimu decoction ,medicine ,Inflammatory factors ,UHPLC-Q-Orbitrap HRMS ,QD1-999 ,Chromatography ,Chemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Gout ,Q exactive ,Gouty arthritis ,Guizhi Shaoyao Zhimu Decoction ,Ultra high performance ,0210 nano-technology ,Chemical components analysis ,Network pharmacology - Abstract
The current study aimed to explore the mechanism of Guizhi Shaoyao Zhimu Decoction in the treatment of gouty arthritis based on the combination of Vanquish Ultra High Performance Liquid Chromatography Q Exactive IV Pole-electrostatic field orbital trap high resolution mass spectrometer (UHPLC-Q-Orbitrap HRMS) and network pharmacology. Firstly, UHPLC-Q-Orbitrap HRMS was established and applied to separation and identification of the chemical components of GSZD. Then, using network pharmacology analysis to obtain the potential active components and candidate targets underlying the effect of GSZD on gouty arthritis (GA). Through targeted and untargeted analysis, a total of 79 components, including 3 chromones, 26 flavonoids, 10 alkaloids, 10 phenolic acids, 7 nucleosides and nucleobases, and 23 other components, were characterized from GSZD. 11 compounds were considered to potentially key active compounds against GA which may be via interacting with multiple inflammatory factors and multiple inflammatory pathways.
- Published
- 2021