Search

Nanostructurization through high-energy mechanochemical milling is reconsidered as an effective method of biomedical engineering of anticancer drugs possessing poor availability in human organisms. In this research, the nanoparticles (NPs) of arsenic sulfides As4S4 combined with ZnS ones prepared by dry milling as As4S4/ZnS nanocomposites or wet milling in water solution of biocompatible copolymer Poloxamer 407 as suspensions are analyzed in respect of compositionally guided anticancer activity. A multiexperimental approach involving both atomic-specific and atomic-deficient methods supported by a structure-morphology study is applied to characterize this NP-based system. A hierarchical model considering free-volume elements between interacting crystallites and their agglomerates is developed to assess positron annihilation lifetime data treated within ×3-term fitting and ×3-×2-coupling decomposition algorithm. A compositionally dependent nanoformulation is represented as a common stabilization of coarse-grained As4S4 and fine-grained ZnS NPs accompanies by positron-to-positronium trapping conversion. An anticancer effect on A375 and Bowes melanoma cell lines clearly confirms the improved biomedical efficacy of the developed As4S4-ZnS nanosystem.