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Ensuring the accuracy and reliability of ventilation parameter monitoring is pivotal for the development of intelligent ventilation systems. To attain a visual representation of airflow, solving the challenge of airflow reconstruction necessitates the strategic use of a limited number of sensors. In addressing these concerns, this article introduces an optimization approach for ventilation airflow leveraging the Breadth-First Search (BFS) algorithm. Additionally, it proposes an optimization distribution method for mine ventilation sensors, grounded in the Independent Cut Set algorithm. Research has found that compared to the traditional PSO algorithm, the BFS algorithm produces a higher optimal air volume solution when optimizing the air volume; Comparatively, the proposed algorithm exhibits significantly shorter average running times than the Particle Swarm Optimization (PSO) algorithm. It boasts the highest average convergence rate, ensuring superior accuracy, and possesses a notable capability to escape local minima, facilitating the acquisition of optimal solutions. Leveraging the independent cut set algorithm optimizes the calculation process through matrix operations. Exploiting the properties of matrices allows for a more rapid and intuitive resolution of sensor localization problems.

Summary: Junctional coupling between endoplasmic reticulum (ER) Ca2+-sensor STIM proteins and plasma membrane (PM) Orai channels mediates Ca2+ signals in most cells. We reveal that PM-tethered, fluorescently tagged C-terminal M4x (fourth transmembrane helix contains a cytoplasmic C-terminal extension) peptides from Orai channels undergo a Leu-specific signature of direct interaction with the STIM1 Orai-activating region (SOAR), exactly mimicking STIM1 binding to gate Orai channels. The 20-amino-acid Orai3-M4x peptide associates avidly with STIM1 within ER-PM junctions, functions to competitively block native Ca2+ signals, and mediates a key modification of STIM-Orai coupling induced by 2-aminoethoxydiphenyl borate. By blocking STIM-Orai coupling, the Orai3-M4x peptide reveals the critical role of Orai channels in driving Ca2+ oscillatory signals and transcriptional control through NFAT. The M4x peptides interact independently with SOAR dimers consistent with unimolecular coupling between Orai subunits and STIM1 dimers. We reveal the critical role of M4x helices in defining the coupling interface between STIM and Orai proteins to mediate store-operated Ca2+ signals.