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Laizhou Bay, a major breeding ground for economic marine organisms in the northern waters of China, is facing rapid environmental degradation. In this study, field surveys in this area were conducted in the spring, summer, and autumn of 2020. Microscopic observation and RuBisCO large subunit (rbcL) gene analysis were employed to understand the community structure and temporal dynamics of phytoplankton. The phytoplankton community structures detected by the two methods showed significant differences. Microscopic observation revealed the dominance of dinoflagellates in spring that shifted to the dominance of diatoms in summer and autumn. However, rbcL gene sequencing consistently identified diatoms as dominant throughout all three seasons, with their relative abundance showing an increasing trend. Conversely, the relative abundance of the second- and third-most abundant taxa, namely, haptophytes and ochrophytes, decreased as the seasons transitioned. rbcL gene sequencing annotated more species than microscopy. It could detect haptophytes and cryptophytes, which were overlooked by microscopy. In addition, rbcL gene sequencing detected a remarkable amount of Thalassiosira profunda, which was previously unidentified in this sea area. However, it appeared to underestimate the contribution of dinoflagellates considerably, with most taxa being only identified through microscopic identification. The two methods jointly identified 28 harmful algal bloom taxa with similar detection quantities but substantial differences in species composition. Phytoplankton communities were influenced by temperature, salinity, and nutrients. The results of this work suggest that a combination of multiple techniques is necessary for a comprehensive understanding of phytoplankton., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Elaborating the environmental behavior of mefentrifluconazole, a novel triazole fungicide, in stereoselective level is of paramount importance for the application of the pesticide in agriculture. In this study, the enantioselective bioactivity, acute toxicity and stereoselective bioaccumulation of mefentrifluconazole in earthworm (Eisenia fetida) were investigated. Bioactivity tests against four pathogens revealed that R-(-)-mefentrifluconazole exhibited approximately 11-113 times higher bioactivity than its S-(+)-mefentrifluconazole. However, the LC 50 of S-(+)-, rac- and R-(-)-mefentrifluconazole to earthworm was measured to be 4.1, 11.4 and 7.3 μg/cm 2 , respectively, indicating active ingredient R-(-)-mefentrifluconazole is less toxic than its racemate and S-form. Accumulation of mefentrifluconazole in earthworms was non-enantioselective and negatively related to its adsorption onto soils. The concentration of mefentrifluconazole in in situ pore water (C IPW ) and CaCl 2 extraction (C CaCl2 ) was closely related to its accumulation in earthworms, suggesting that C IPW and C CaCl2 could be appropriate indicators for estimation of the bioavailability of mefentrifluconazole in soil. Conclusively, our study provides necessary information for the risk assessment of mefentrifluconazole in agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Triazole-resistance in Aspergillus fumigatus is widespread. We evaluated whether triazole-resistance in A. fumigatus and its related cyp51A mutations, induced by a combination of the triazole fungicides difenoconazole and propiconazole, differs from resistance induced by the individual fungicides. Both difenoconazole and propiconazole can induce triazole-resistance in A. fumigatus. Resistance is much easier induced by formulated fungicides or a combination of these two fungicides compared with standard fungicides or individual fungicides, respectively. Six different mutations (G138S, G138D, H147Y, I246M, M263I and D430N) were identified in the induced resistant strains. The H147Y, I246M and M263I mutations were associated with triazole-resistance. This implies that the application of a combination of difenoconazole and propiconazole may result in higher triazole-resistance in A. fumigatus and more mutations in the cyp51A gene., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Residual acetochlor and atrazine in soils, resulting from their extensive application to maize plants, may affect product safety of the ultimate wheat crop. To determine the potential uptake and accumulation of acetochlor and atrazine by wheat plants, the uptake mechanism, translocation, and subcellular distribution of these two herbicides were studied through hydroponic experiments (10 mg L - 1 ). The results indicated that acetochlor can be taken up through the apoplastic pathway and can accumulate in wheat roots with little upward translocation. However, atrazine could be taken up by roots through the symplastic pathway and subsequently transported to the stems and leaves. Little upward translocation of acetochlor in wheat plants was due to its preferential distribution into root organelles with higher lipid contents. Conversely, the low bioconcentration of atrazine in root organelles and cell walls after uptake led to its easy upward translocation. Uptake of acetochlor and atrazine by wheat roots and the distribution of atrazine to the stems and leaves were predicted well by using the partition-limited model. The obtained results indicated that residual atrazine in soil may be taken up by wheat roots and acropetally translocated, thereby posing a threat to product safety of wheat., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The existing form of an ionizable organic compound can simultaneously affect its soil adsorption and plant bioactivity. In this experiment, the adsorption and bioactivity of two weak acid herbicides (WAHs), imazethapyr and 2,4-D, were studied to explore the predominant mechanism by which the soil pH and the addition of biochar can influence the phytotoxicity of WAHs in soil. Then, the WAH concentration extracted by hollow fiber-based liquid-phase microextraction (C HF-LPME ), the in situ pore water concentration (C IPW ) and the added concentration (C AC ) were employed to estimate the phytotoxicity. The results showed that with increased pH from 5.5 to 8.5, the phytotoxicity of the WAHs to rice increased about 1-fold in the soil, but decreased in aqueous solutions, the IC 50 values for imazethapyr and 2,4-D at pH 5.0 were 3- and 2-fold higher than that at pH 8.0. In addition, the soil adsorption decreased, indicating that the adsorption process was the dominant factor for the variation of the phytotoxicity of the WAHs in the tested soil instead of the decreasing bioactivity. The concentration that inhibits plant growth by 50% (IC 50 ) calculated by the C AC in different pH and biochar soils ranged from 0.619 to 3.826 mg/kg for imazethapyr and 1.871-72.83 mg/kg for 2,4-D. The coefficient of variation (CV) of the IC 50 values reached 65.61% for imazethapyr and 130.0% for 2,4-D. However, when IC 50 was calculated by C IPW and C HF-LPME , the CVs of the IC 50 values decreased to 23.51% and 36.23% for imazethapyr and 40.21% and 50.93% for 2,4-D, respectively. These results suggested that C IPW and C HF-LPME may be more appropriate than C AC for estimating the phytotoxicity of WAHs., (Copyright © 2018 Elsevier Inc. All rights reserved.)