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The effects of exposure to the environmental toxicant cadmium, in combination with obesity, on the metal content in mouse testis were evaluated. Starting in utero and continuing through to 10 or 24 weeks post-weaning, male mice were exposed to cadmium (0, 0.5 or 5 ppm), and fed either a low (LFD) or high fat diet (HFD) post-weaning. Testicular levels of cadmium and essential metals were determined 10 and 24 weeks post-weaning by ICP-MS. Similar to what has been previously observed in the liver, kidney, heart and brain, significant levels of cadmium accumulated in the testis under all exposure conditions. Additionally, HFD-fed animals accumulated more cadmium than did their LFD-treated counterparts. Both treatments affected essential metal homeostasis in the testis. These findings suggest that cadmium and obesity may compromise the reproductive potential in the male mouse by disrupting essential metal levels.

Background: Coronary atherosclerosis (CAS) is a complex chronic inflammatory disease with significant genetic and environmental contributions. While genome-wide association studies (GWAS) have pinpointed many risk loci, over 75 % are in non-coding regions, complicating functional analysis and understanding gene-disease mechanisms. Methods: We conducted a cross-tissue transcriptome-wide association study (TWAS) using data from the GWAS Catalog (16,041 cases, 440,307 controls) and the Genotype-Tissue Expression (GTEx) v8 eQTL dataset. Initially, we used the Unified Test for Molecular Signatures (UTMOST) for analysis, followed by validation with Functional Summary-based Imputation (FUSION) and conditional and joint (COJO) analyses. Candidate genes were further refined using Multi-marker Analysis of Genomic Annotation (MAGMA). Causal relationships were assessed through Summary Data-Based Mendelian Randomization (SMR), colocalization analysis (COLOC), and Mendelian Randomization (MR). GeneMANIA was used to identify interacting genes, and Phenome-Wide Association Study (PheWAS) was employed to enhance the results. Results: UTMOST identified 33 susceptibility genes for CAS. Out of these, 17 met stringent criteria in both UTMOST and FUSION analyses. Combining results from UTMOST, FUSION, and MAGMA, we identified four critical candidate genes. WDPCP was the only gene to pass SMR, COLOC, and MR analyses, confirming its causal role in CAS. GeneMANIA revealed additional interacting genes, and PheWAS validated WDPCP's role as a susceptibility gene. Conclusion: WDPCP is a potential novel susceptibility gene for CAS, influencing endothelial function, lipid metabolism, and coronary artery development. This study extends GWAS findings, highlighting WDPCP's potential as a therapeutic target and its consistent expression across different tissues. Further validation studies are warranted.

Afforestation is recognized as a crucial strategy for mitigating global warming. Therefore, appropriate areas and scales of afforestation needed to be accurate identify and plan, due to the varying levels of economic development and ecological protection across different regions and future scenarios. This study utilized land use data, natural and socio-economic data as the indicators from 2000 to 2020. The Patch-level Land Use Simulation Model (PLUS) was employed to simulate and analyze afforestation potential areas (APAs) under four scenarios: Natural Development (NDS), Ecological Protection (EPS), Urban Development (UDS), and Cultivated Land Protection (CLPS) for the years 2035 and 2050. The results shown that: (1) the spatial distribution of APAs in Yunnan Province under different scenarios shown significant spatial heterogeneity. The APAs are mainly concentrated in economically backward areas with favorable natural conditions. Moreover, the APAs are primarily found around existing forests. (2) The EPS scenario revealed the highest afforestation potential, with 14,132.14 km2 and 13,825.76 km2 of high-APAs, and 5,204.17 km2 and 5,262.4 km2 of low-APAs expected by 2035 and 2050, respectively. The CLPS scenario shown the lowest afforestation potential. (3) The APAs are mainly influenced by natural conditions, the strictness of forest management, and the level of local economic development. Climate and soil, as fundamental natural elements, constitute the foundational conditions for these potential areas. (4) Afforestation strategies should have emphasized ’adapting to local conditions and time’ at different development stages. The program would be dynamically adjusted according to future development scenarios to maximize afforestation potential areas and ecological value. These strategies could provide valuable suggestions for future afforestation planning in Yunnan Province.

Background/Objective: This study assessed the correlation between latitude and the cardiorespiratory fitness (CRF) of children and adolescents. Methods: In 16 provinces and autonomous regions in China, 25,941 children and adolescents aged 10–18 were included. CRF was measured using the 20 m shuttle run test (20 m SRT) and estimated peak oxygen uptake (VO2peak). One-way ANOVA and multiple regression analysis were used to explore the correlation between CRF and latitude in children and adolescents. Results: The VO2peak values of the low (south), middle, and high (north) latitude groups for boys were 43.1, 43.1, and 40.7 mL/kg/min, respectively, and 40.0, 40.0, and 38.5 mL/kg/min for girls, respectively. After adjusting for confounding factors, the regression coefficients (β) between VO2peak-Z and both latitude-Z and (latitude-Z)2 for boys were −0.151 and −0.043, respectively. For girls, they were −0.142 and −0.020, respectively. The Partial correlation coefficient (r) for latitude-Z and (latitude-Z)2 were −0.14 and −0.04 for boys, and −0.13 and −0.02 for girls, respectively. Conclusion: The CRF among children and adolescents in high latitude regions is significantly lower than that in middle and low latitude region, and it generally shows a “parabolic” trend between Latitude-Z and VO2peak-Z.

Phenolic moieties are important constituents in dissolved organic matter (DOM) in natural and engineered systems. However, their roles in membrane fouling mechanism during drinking water treatment by ultrafiltration (UF) have remained elusive. Herein, by using water insoluble polyvinylpolypyrrolidone (PVPP) resins, we sequestered the phenolic moieties from a model DOM (Suwannee River DOM, SRDOM) and characterized their molecular profiles using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS). Subsequently, their roles in UF membrane fouling propensity were investigated using reconstituted DOM solutions with various concentrations of phenolic moieties. The results showed that the phenolic moieties were of higher molecular weight and rich in unsaturation cyclic structures and oxygen-rich groups. Van Krevelen diagrams revealed that the sequestered sample was rich in aromatics structures and tannins-like compounds while contained less alicyclic organic acids in comparison with the original SRDOM, which was consistent with the aromaticity index (AI) analysis. UF experiments showed that the more phenolic moieties in DOM solution, the severer decline of flux was observed. The phenolic moieties played a significant role in membrane irremovable fouling due to the hydrophobic interactions and their higher molecular weight as evidenced by membrane cleaning tests. By surface characterization, the SRDOM fouled membrane was identified to have a higher water contact angle value and abundant C–O groups, likely due to the adsorption of more hydrophobic phenolic moieties. Overall, these findings highlighted links between phenolic moieties and membrane fouling development, and implied that membrane performance could be improved by pre-removal of phenolic moieties in DOM. Keywords: Membrane fouling, Dissolved organic matter, Polyvinylpolypyrrolidone, Phenolic moieties, Electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry