5 results on '"Wittkopp PJ"'
Search Results
2. Mechanisms of regulatory evolution in yeast.
- Author
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Siddiq MA and Wittkopp PJ
- Subjects
- Genome, Saccharomyces cerevisiae genetics, Evolution, Molecular
- Abstract
Studies of regulatory variation in yeast - at the level of new mutations, polymorphisms within a species, and divergence between species - have provided great insight into the molecular and evolutionary processes responsible for the evolution of gene expression in eukaryotes. The increasing ease with which yeast genomes can be manipulated and expression quantified in a high-throughput manner has recently accelerated mechanistic studies of cis- and trans-regulatory variation at multiple evolutionary timescales. These studies have, for example, identified differences in the properties of cis- and trans-acting mutations that affect their evolutionary fate, experimentally characterized the molecular mechanisms through which cis- and trans-regulatory variants act, and illustrated how regulatory networks can diverge between species with or without changes in gene expression., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
3. The Genetic Basis of Pigmentation Differences Within and Between Drosophila Species.
- Author
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Massey JH and Wittkopp PJ
- Subjects
- Abdomen physiology, Animals, Organ Specificity genetics, Pupa genetics, Thorax physiology, Wings, Animal physiology, Drosophila genetics, Pigmentation genetics
- Abstract
In Drosophila, as well as in many other plants and animals, pigmentation is highly variable both within and between species. This variability, combined with powerful genetic and transgenic tools as well as knowledge of how pigment patterns are formed biochemically and developmentally, has made Drosophila pigmentation a premier system for investigating the genetic and molecular mechanisms responsible for phenotypic evolution. In this chapter, we review and synthesize findings from a rapidly growing body of case studies examining the genetic basis of pigmentation differences in the abdomen, thorax, wings, and pupal cases within and between Drosophila species. A core set of genes, including genes required for pigment synthesis (eg, yellow, ebony, tan, Dat) as well as developmental regulators of these genes (eg, bab1, bab2, omb, Dll, and wg), emerge as the primary sources of this variation, with most genes having been shown to contribute to pigmentation differences both within and between species. In cases where specific genetic changes contributing to pigmentation divergence were identified in these genes, the changes were always located in noncoding sequences and affected cis-regulatory activity. We conclude this chapter by discussing these and other lessons learned from evolutionary genetic studies of Drosophila pigmentation and identify topics we think should be the focus of future work with this model system., (© 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
4. The function and regulation of Ultrabithorax in the legs of Drosophila melanogaster.
- Author
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Davis GK, Srinivasan DG, Wittkopp PJ, and Stern DL
- Subjects
- Animals, Base Sequence, Body Patterning genetics, DNA genetics, Drosophila genetics, Drosophila Proteins genetics, Drosophila melanogaster genetics, Enhancer Elements, Genetic, Evolution, Molecular, Extremities growth & development, Female, Gene Expression Regulation, Developmental, Genes, Homeobox, Genes, Insect, Genetic Complementation Test, Homeodomain Proteins genetics, Male, Mutation, Recombination, Genetic, Species Specificity, Transcription Factors genetics, Drosophila Proteins physiology, Drosophila melanogaster growth & development, Drosophila melanogaster physiology, Homeodomain Proteins physiology, Transcription Factors physiology
- Abstract
Alterations in Hox gene expression patterns have been implicated in both large and small-scale morphological evolution. An improved understanding of these changes requires a detailed understanding of Hox gene cis-regulatory function and evolution. cis-regulatory evolution of the Hox gene Ultrabithorax (Ubx) has been shown to contribute to evolution of trichome patterns on the posterior second femur (T2p) of Drosophila species. As a step toward determining how this function of Ubx has evolved, we performed a series of experiments to clarify the role of Ubx in patterning femurs and to identify the cis-regulatory regions of Ubx that drive expression in T2p. We first performed clonal analysis to further define Ubx function in patterning bristle and trichome patterns in the legs. We found that low levels of Ubx expression are sufficient to repress an eighth bristle row on the posterior second and third femurs, whereas higher levels of expression are required to promote the development and migration of other bristles on the third femur and to repress trichomes. We then tested the hypothesis that the evolutionary difference in T2p trichome patterns due to Ubx was caused by a change in the global cis-regulation of Ubx expression. We found no evidence to support this view, suggesting that the evolved difference in Ubx function reflects evolution of a leg-specific enhancer. We then searched for the regulatory regions of the Ubx locus that drive expression in the second and third femur by assaying all existing regulatory mutations of the Ubx locus and new deficiencies in the large intron of Ubx that we generated by P-element-induced male recombination. We found that two enhancer regions previously known to regulate Ubx expression in the legs, abx and pbx, are required for Ubx expression in the third femur, but that they do not contribute to pupal expression of Ubx in the second femur. This analysis allowed us to rule out at least 100 kb of DNA in and around the Ubx locus as containing a T2p-specific enhancer. We then surveyed an additional approximately 30 kb using enhancer constructs. None of these enhancer constructs produced an expression pattern similar to Ubx expression in T2p. Thus, after surveying over 95% of the Ubx locus, we have not been able to localize a T2p-specific enhancer. While the enhancer could reside within the small regions we have not surveyed, it is also possible that the enhancer is structurally complex and/or acts only within its native genomic context.
- Published
- 2007
- Full Text
- View/download PDF
5. Immunohistochemical colocalization of Yellow and male-specific Fruitless in Drosophila melanogaster neuroblasts.
- Author
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Radovic A, Wittkopp PJ, Long AD, and Drapeau MD
- Subjects
- Animals, Female, Immunohistochemistry, Male, Sex Factors, Sexual Behavior, Animal, Transcription, Genetic, Drosophila Proteins, Drosophila melanogaster metabolism, Insect Proteins biosynthesis, Nerve Tissue Proteins biosynthesis, Neurons metabolism, Transcription Factors biosynthesis
- Abstract
The Drosophila melanogaster fruitless gene encodes multiple male-specific transcription factors that are hypothesized to regulate a hierarchy of genes responsible for the development of male courtship behavior. Here we show that there are dramatically increased levels of the protein product of the male courtship behavior gene yellow associated with male-specific Fruitless protein in a subset of neuroblasts in third-instar larval male brains. We hypothesize that yellow is downstream of fruitless in a male courtship behavior developmental genetic pathway., ((c) 2002 Elsevier Science (USA).)
- Published
- 2002
- Full Text
- View/download PDF
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