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Unlabelled: Oral cancer disease represents a significant fraction of all human cancer types and its poor early diagnosis contributes to reduced individual survival rate. The identification of proteins modulated in tumorigenic cells and its post-translational modifications may improve our understanding of tumor development in epithelial cells. We have analyzed the phosphoproteome of tumorigenic (SCC-9) and non-tumorigenic (HaCaT) cell lines using MS-based approach in order to identify phosphopeptides with differing patterns of modifications and/or abundance. Our results revealed the identity of 4,206 protein phosphorylation sites with sixty-two sites showing to be significantly modulated between the two cell lines. The phosphoproteome data showed an overrepresentation of proteins with a possible role in nuclear regulatory functions. Pathway analysis was further performed on the phosphoproteome dataset and differences and commonalities of the functional pathways present in tumorigenic and non-tumorigenic cells were identified. Phosphopeptides that belong to the proteins lamina-associated polypeptide 2 isoform alpha and serine-arginine repetitive matrix protein 2 were identified with differential abundance and they appear as promising tumor-related phosphopeptides. These two proteins may be related to the structural alterations generally found in the nucleus of tumorigenic cells. The identification of phosphorylation sites in tumorigenic cells may contribute to disclose novel signaling mechanisms associated with OSCC., Significance: Oral Squamous Cell Carcinoma (OSCC) is an important cancer disease affecting thousands of people worldwide. Many cellular processes related to the development of oral cancer remain unknown; however, the studies performed in vitro with cancer cells have contributed to guide more specific research which may be further performed by using in vivo approaches or clinical samples. To our knowledge, only few studies have been published showing the results of phosphoproteome profiling of squamous cell carcinoma models, and many signaling proteins must be identified and functionally characterized in order to increase the knowledge available about the complexity of the signaling networks responsible for oral cancer development and its progression. Furthermore, our knowledge regarding proteins exclusive or very low abundant in cancer cells remains limited. A better understanding of the differences between signaling pathways present in epithelial cell lines may contribute to reveal the processes underlying the OSCC., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Understanding the processes and mechanisms of carbon acquisition and accumulation in microalgae is fundamental to enhance the cellular capabilities aimed to environmental and industrial applications. The "omics" approaches have greatly contributed to expanding the knowledge on these carbon-related cellular responses, reporting large data sets on microalgae transcriptome, proteome and metabolome. This review emphasizes the advances made on Chlamydomonas exploration; however, some knowledge acquired from studying this model organism, may be extrapolated to close algae species. The large data sets available for this organism revealed the identity of a vast range of genes and proteins which are integrating carbon-related mechanisms. Nevertheless, these data sets have also highlighted the need for integrative analysis in order to fully explore the information enclosed. Here, some of the main results from "omics" approaches which may contribute to the understanding of carbon acquisition and accumulation in Chlamydomonas were reviewed and possible applications were discussed., Biological Significance: A number of important publications in the field of "omics" technologies have been published reporting studies of the model green microalga Chlamydomonas reinhardtii and related to microalgal biomass production. However, there are only few attempts to integrate these data. Publications showing the results from "omics" approaches, such as transcriptome, metabolome and proteome, focused in the study of mechanisms of carbon acquisition and accumulation in microalgae were reviewed. This review contributes to highlight the knowledge recently generated on such "omics" studies and it discusses how these results may be important for the advance of applied sciences, such as microalgae biotechnology., (© 2013.)

Leishmania amazonensis causes a wide spectrum of leishmaniasis. There are no vaccines or adequate treatment for leishmaniasis, therefore there is considerable interest in the identification of new targets for anti-leishmania drugs. The central role of telomere-binding proteins in cell maintenance makes these proteins potential targets for new drugs. In this work, we used a combination of purification chromatographies to screen L. amazonensis proteins for molecules capable of binding double-stranded telomeric DNA. This approach resulted in the purification of a 38kDa polypeptide that was identified by mass spectrometry as Rbp38, a trypanosomatid protein previously shown to stabilize mitochondrial RNA and to associate with nuclear and kinetoplast DNAs. Western blotting and supershift assays confirmed the identity of the protein as LaRbp38. Competition and chromatin immunoprecipitation assays confirmed that LaRbp38 interacted with kinetoplast and nuclear DNAs in vivo and suggested that LaRbp38 may have dual cellular localization and more than one function.