1. An essential role for Cmtr2 in mammalian embryonic development.
- Author
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Yermalovich AV, Mohsenin Z, Cowdin M, Giotti B, Gupta A, Feng A, Golomb L, Wheeler DB, Xu K, Tsankov A, Cleaver O, and Meyerson M
- Subjects
- Animals, Female, Mice, Pregnancy, Cell Proliferation, Embryo, Mammalian metabolism, Endothelial Cells metabolism, Mice, Knockout, Placenta metabolism, Signal Transduction, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 genetics, Yolk Sac metabolism, Yolk Sac embryology, Embryonic Development genetics, Methyltransferases metabolism, Methyltransferases genetics
- Abstract
CMTR2 is an mRNA cap methyltransferase with poorly understood physiological functions. It catalyzes 2'-O-ribose methylation of the second transcribed nucleotide of mRNAs, potentially serving to mark RNAs as "self" to evade the cellular innate immune response. Here we analyze the consequences of Cmtr2 deficiency in mice. We discover that constitutive deletion of Cmtr2 results in mouse embryos that die during mid-gestation, exhibiting defects in embryo size, placental malformation and yolk sac vascularization. Endothelial cell deletion of Cmtr2 in mice results in vascular and hematopoietic defects, and perinatal lethality. Detailed characterization of the constitutive Cmtr2 KO phenotype shows an activation of the p53 pathway and decreased proliferation, but no evidence of interferon pathway activation. In summary, our study reveals the essential roles of Cmtr2 in mammalian cells beyond its immunoregulatory function., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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