1. A genetic analysis of the adenine phosphoribosyl transferase locus in Chinese hamster V79-AP4 cells: relevance to mutagenesis studies.
- Author
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Colella CM, Simi S, Van Boxel T, Talarico D, Della Valle G, Carrozza ML, Fratta D, Mariani T, Piras A, and Simili M
- Subjects
- 2-Aminopurine analogs & derivatives, 2-Aminopurine pharmacology, Animals, Blotting, Southern, Cell Line, Chromosome Banding, Chromosome Mapping, DNA Probes, Isoenzymes, L-Lactate Dehydrogenase genetics, Adenine Phosphoribosyltransferase genetics, Cricetinae genetics, Cricetulus genetics, Mutagenicity Tests methods, Pentosyltransferases genetics
- Abstract
The chromosomal location of the autosomal locus aprt has been investigated in the permanent Chinese hamster cell line V79-AP4 by standard somatic cell genetics methodologies. Aprt is functionally dizygous in V79-AP4 and the 2 alleles map on 2 chromosome 3 homologs, in agreement with the chromosome assignment of the gene in Chinese hamster primary cells. Chromosome G-banding and a Southern blot analysis of V79-AP4 DNA, using as a probe the cloned Chinese hamster aprt gene, have not revealed any structural alteration at either of the 2 aprt alleles. One of the chromosomes 3 has, however, a terminal deletion in its long arm and is therefore morphologically marked. These findings could make V79-AP4 an interesting cell system for the study of mutational mechanisms at the aprt locus in Chinese hamster.
- Published
- 1988
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