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2 results on '"Tomasini, Nicolás"'

1. Trypanosoma cruzi I haplogroups in american countries based on spliced leader-intergenic spacer polimorphism

Author

Cura, Carolina Inés, Mejía Jaramillo, Ana M., Duffy, Tomás, Burgos, Juan Miguel, Rodriguero, Marcela Silvina, Cardinal, Marta Victoria, Kjos, Sonia, Gurgel Gonçalves, Rodrigo, Blanchet, Denis, De Pablos, Luis M., Tomasini, Nicolás, da Silva, Alexandre, Russomando, Graciela Mabel, Cuba, Cesar A. Cuba, Aznar, Christine, Abate, Teresa, Levin, Mariano Jorge, Osuna, Antonio, Gurtler, Ricardo Esteban, Diosque, Patricio, Solari, Aldo, Triana Chávez, Omar, and Schijman, Alejandro Gabriel

Subjects
Ciencias Biológicas, purl.org/becyt/ford/1 [https], PCR, DISCRETE TYPING UNIT, Otras Ciencias Biológicas, CHAGAS DISEASE, SPLICED-LEADER INTERGENIC REGION, purl.org/becyt/ford/1.6 [https], CIENCIAS NATURALES Y EXACTAS, GENOTYPE, TRYPANOSOMA CRUZI I
Abstract

The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motif proposed by Herrera et al. (2007) to define four haplotypes in Colombia, we could classify these genotypes into four distinct Tc I SL-IR groups, three corresponding to the former haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and one novel group, Ie (19 genotypes). Genotypes harbouring the Tc Ic motif were not detected in our study. Tc Ia was associated with domestic cycles in southern and northern South America and sylvatic cycles in Central and North America. Tc Ib was found in all transmission cycles from Colombia. Tc Id was identified in all transmission cycles from Argentina and Colombia, including Chagas cardiomyopathy patients, sylvatic Brazilian samples and human cases from French Guiana, Panama and Venezuela. Tc Ie gathered five samples from domestic Triatoma infestans from northern Argentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and two chagasic patients from Chile and one from a Bolivian patient with chagasic reactivation. Mixed infections by Tc Ia. +. Tc Id, Tc Ia. +. Tc Ie and Tc Id. +. Tc Ie were detected in vector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Id were identified in different tissues from a heart transplanted Chagas cardiomyopathy patient with reactivation, denoting histotropism. Trypanosoma cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphis virginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodnius neglectus from Brazil and M. spinolai and M. gajardoi from Chile are to our knowledge described for the first time. Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Mejía Jaramillo, Ana M.. Universidad de Antioquia; Colombia Fil: Duffy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Burgos, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Kjos, Sonia. Centers for Disease Control and Prevention; Estados Unidos Fil: Gurgel Gonçalves, Rodrigo. Universidade do Brasília; Brasil Fil: Blanchet, Denis. Universite Des Antilles Et de la Guyane; Guayana Francesa Fil: De Pablos, Luis M.. Universidad de Granada; España Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: da Silva, Alexandre. Centers for Disease Control and Prevention; Estados Unidos Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay Fil: Cuba, Cesar A. Cuba. Universidade do Brasília; Brasil Fil: Aznar, Christine. Universite Des Antilles Et de la Guyane; Guayana Francesa Fil: Abate, Teresa. Universidad Central de Venezuela. Facultad de Medicina; Venezuela Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Osuna, Antonio. Universidad de Granada; España Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Solari, Aldo. Facultad de Medicina de la Universidad de Chile; Chile Fil: Triana Chávez, Omar. Universidad de Antioquia; Colombia Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina

Published
2010

2. Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina.

Author

Ragone PG, Pérez Brandán C, Padilla AM, Monje Rumi M, Lauthier JJ, Alberti D'Amato AM, Tomasini N, Cimino RO, Romero NM, Portelli M, Nasser JR, Basombrío MA, and Diosque P

Subjects
Animals, Antibodies, Protozoan blood, Argentina epidemiology, Blood parasitology, Chagas Disease immunology, Chagas Disease pathology, DNA Fingerprinting, DNA, Protozoan genetics, Disease Models, Animal, Enzymes analysis, Genetic Variation, Heart parasitology, Humans, Male, Mice, Mice, Inbred C57BL, Muscle, Skeletal parasitology, Muscle, Skeletal pathology, Myocardium pathology, Trypanosoma cruzi classification, Trypanosoma cruzi isolation & purification, Chagas Disease epidemiology, Chagas Disease parasitology, Endemic Diseases, Trypanosoma cruzi pathogenicity
Abstract

The biological behavior of the different Trypanosoma cruzi strains is still unclear and the importance of exploring the relevance of these differences in natural isolates is of great significance. Herein we describe the biological behavior of four T. cruzi isolates circulating sympatrically in a restricted geographic area in Argentina endemic for Chagas Disease. These isolates were characterized as belonging to the Discrete Typing Units (DTUs) TcI, TcIII, TcV and TcVI as shown by Multilocus Enzyme Electrophoresis and Multilocus Sequence Typing. In order to study the natural behavior of the different isolates and to preserve their natural properties, we developed a vector transmission model that allows their maintenance in the laboratory. The model consisted of serial passages of these parasites between insect vectors and mice. Vector-derived parasite forms were then inoculated in C57BL/6J mice and number of parasite in peripheral blood, serological response and histological damage in acute and chronic phases of the infection were measured. Parasites from DTUs TcI, TcIII and TcVI were detected by direct fresh blood examination, while TcV parasites could only be detected by Polimerase Chain Reaction. No significant difference in the anti-T. cruzi antibody response was found during the chronic phase of infection, except for mice infected with TcV parasites where no antibodies could be detected. Histological sections showed that TcI isolate produced more damage in skeletal muscle while TcVI induced more inflammation in the heart. This work shows differential biological behavior among different parasite isolates obtained from the same cycle of transmission, permitting the opportunity to formulate future hypotheses of clinical and epidemiological importance., (Copyright © 2012 Elsevier B.V. All rights reserved.)

Published
2012
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