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Background and Aim: Atrial fibrillation (AF) is the most common sustained arrhythmia in hypertrophic cardiomyopathy (HCM) with significant effects on outcome. We aim to compare the left atrial (LA) diameter measurement with HCM-AF Score in predicting atrial fibrillation (AF) development in HCM., Methods: From the regional cohort of the Campania Region, Italy, 519 HCM patients (38% women, age45 ± 17 years) without history of AF, were enrolled in the study. The primary clinical endpoint was the development of AF, defined as at least 1 episode documented by ECG., Results: During the follow-up (mean 8 ± 6, IQ range 2.5-11.2 years), 99 patients (19%) developed AF. Patients who developed AF were more symptomatic, had higher prevalence of ICD implantation, had larger LA diameter, greater left ventricular (LV) maximal wall thickness and LV outflow tract obstruction (p < 0.01). Both LA diameter and HCM-AF score were higher in patients who developed AF versus those who did not (LA diameter 49 ± 7 versus 43 ± 6 mm; HCM-AF score 22 ± 4 versus 19 ± 4; p < 0.0001); however, ROC curve analysis demonstrated that LA diameter had a significant greater area under the curve than HCM-AF Score (p < 0.0001). At 5 years follow-up, a LA diameter > 46 mm, showed a similar accuracy in predicting AF development of HCM-AF score ≥ 22, which identifies patients at high risk to develop AF., Conclusion: Our analysis shows that LA diameter, a worldwide and simple echocardiographic measure, is capable alone to predict AF development in HCM patients., (Copyright © 2023 Elsevier Ireland Ltd. All rights reserved.)

Background: Abl1 is a protein tyrosine kinase whose aberrant activation due to mutations is the culprit of several cancers, most notably chronic myeloid leukaemia. Several Abl1 inhibitors are used as anti-cancer drugs. Unfortunately, drug resistance limits their effectiveness. The main cause for drug resistance is mutations in the kinase domain (KD) of Abl1 that evolve in patients. The T315I mutation confers resistance against all clinically-available inhibitors except ponatinib. Resistance to ponatinib can develop by compound (double) mutations., Methods: Kinetic measurements of the KD of Abl1 and its mutants were carried out to examine their catalytic activity. Specifically, mutants that lead to drug resistance against ponatinib were considered. Molecular dynamics simulations and multiple sequence analysis were used for explanation of the experimental findings., Results: The catalytic efficiency of the T315I pan-resistance mutant is more than two times lower than that of the native KD. All ponatinib resistant mutations restore the catalytic efficiency of the enzyme. Two of them (G250E/T315I and Y253H/E255V) have a catalytic efficiency that is more than five times that of the native KD., Conclusions: The measurements and analysis suggest that resistance is at least partially due to the development of a highly efficient kinase through subsequent mutations. The simulations highlight modifications in two structurally important regions of Abl1, the activation and phosphate binding loops, upon mutations., General Significance: Experimental and computational methods were used together to explain how mutations in the kinase domain of Abl1 lead to resistance against the most advanced drug currently in use to treat chronic myeloid leukaemia., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Over the last decades, traditional olive production has been converted to intensive and super-intensive cultivation systems, characterized by high plant density and irrigation. Although this conversion improves product quality and quantity, it requires a larger amount of energy input. The new contributions in this paper are, first, an analysis of the energy and environmental performance of two commercial-scale high peak-power hybrid photovoltaic irrigation systems (HPVIS) installed at intensive and super-intensive Mediterranean olive orchards; second, an analysis of PV hybrid solutions, comparing PV hybridization with the electric power grid and with diesel generators; and finally, a comparison of the environmental benefits of HPVIS with conventional power sources. Energy and environmental performances were assessed through energy and carbon payback times (EPBT and CPBT). The results show EPBT of 1.98 and 4.58 years and CPBT of 1.86 and 9.16 years for HPVIS in Morocco and Portugal, respectively. Moreover, the HPVIS were able to achieve low emission rates, corresponding to 48 and 103 g CO 2 e per kWh generated. The EPBT and CPBT obtained in this study were directly linked with the irrigation schedules of the olive orchards; therefore, weather conditions and irrigation management may modify the energy and environmental performances of HPVIS. The consumption of grid electricity and diesel fuel, before and after the implementation of HPVIS, was also analyzed. The results obtained show fossil energy savings of 67% for the Moroccan farm and 41% for the Portuguese installation. These savings suggest that the energy produced by HPVIS in olive orchards will avoid the emissions of a large amount of greenhouse gas and the exploitation of natural resources associated with fossil fuel production., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)

The setting up of innovative irrigation water management might contribute to the mitigation of negative issues related to climate change. Our hypothesis was that globe artichoke irrigated with a traditionally drip system could be converted to an innovative water management system based on precision irrigation techniques and on evaporative cooling application in order to improve crop physiological status with positive impacts on earliness, total heads yield and water saving. Over two experiments carried out at plot- and field-scale, two irrigation management systems, differing in type and application time, were compared: (i) conventional, and (ii) canopy-cooling. Plant physiological status at a weekly sampling interval and the head atrophy incidence (as the ratio of the total primary heads collected) were monitored. We also recorded and determined heads production, and yield components. In both experiments, throughout the application period of evaporative cooling (three months), canopy-cooling showed the lowest value of leaf temperature and the highest photosynthesis values compared with the conventional one (+3 °C and -30%, respectively). The physiological advantage gained by the crop with evaporative cooling has led to a higher production both in terms of total yield (+30%), and in terms of harvested first order heads that from an economic viewpoint are the most profitable for farmers. At farm-scale, the canopy-cooling treatment resulted in a higher earliness (35 days) and water productivity (+36%) compared with conventional one. Our findings show that by combining evaporative cooling practice with precision irrigation technique the heads yield can be optimized also leading to a relevant water saving (-34%). Moreover, the study proved that canopy-cooling set up might be a winning strategy in order to mitigate climatic changes and heat stress conditions., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)