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Ammonia is a promising fuel with high energy density, accessible storage, and no CO 2 production by combustion, but its combustion produces the pollutant NO. In this study, a Bunsen burner experimental bench was selected to investigate the concentration of NO generated by ammonia combustion at different initial oxygen concentrations. Further, the reaction pathways of NO were analyzed in depth, and sensitivity analysis was performed. The results show that the Konnov mechanism has an excellent predictive effect on NO generated by ammonia combustion. In the ammonia-premixed laminar flame at atmospheric pressure, the NO concentration peaked at an equivalence ratio of 0.9. The high initial oxygen concentration enhanced the combustion of ammonia-premixed flame and increased the conversion of NH 3 to NO. NO was not only a product but a contribution to the combustion of NH 3 . As the equivalence ratio increases, NH 2 consumes a large amount of NO and reduces NO production. The high initial oxygen concentration enhanced NO production, and the effect was more pronounced at low equivalents. The study results provide theoretical guidance for the utilization of ammonia combustion and pollutant reduction and help to drive the process of ammonia combustion toward practicality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)