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The properties of chitin-based adsorbents varied among studies since they are influenced by different factors, such as the types of base and acid used to extract the chitin. Therefore, this works aimed to investigate the impact of four different acid solutions on the extraction and properties of chitin from shrimp shell waste, and to evaluate the adsorption performance of the obtained chitin on removing dye from an aqueous solution. The result showed that H 2 SO 4 , HCl, and HNO 3 could remove high minerals from the shrimp shell, while the effect of CH 3 COOH was inferior. The Fourier Transform Infrared (FTIR) and X-ray diffraction (XRD) indicated that the extracted chitin was α-amorphous structure, regardless of the type of acid solution. However, the type of acid solution influenced the crystallinity index of the extracted chitin. The Scanning Electron Microscope (SEM) showed both fibrillar material and porous structures. In addition, the chitin extracted through demineralization using H 2 SO 4 was more effective in removing RBBR dye from aqueous solution, followed by HCl, HNO 3, and the last, CH 3 COOH treatment. The performances of chitin-based adsorbent could be attributed to the strength of acid solution used to remove mineral during the extraction process and the obtained pore structures., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The main obstacles on silver rasbora (Rasbora argyrotaenia) culture are having the limited number of broodstock and spawning depending on the season. The purpose of this study was to determine the effect of different dosage of Ovaprim™ induction by topical gill method to silver rasbora spermiation in order to continue the production out of its reproduction season with an optimum dose. A total of 30 male fish with a weight of 7.78 ± 0.20 g and length 4.11 ± 0.31 cm was used in this research. Topical gill treatments of Ovaprim™ were administered with following doses; 0.15 μl/g, 0.25 μl/g, 0.35 μl/g, 0.45 μl/g and 0.55 μl/g body weight. Milt volume, sperm concentration, sperm motility, and sperm viability parameters were observed in this study to understand the optimum dose of Ovaprim™ for male silver rasbora breeders. Spermiation induction of silver rasbora using Ovaprim™ with topical gill method has been successfully carried out, indicating an increase (P < 0.05) in milt volume, sperm concentration, sperm motility, and sperm viability. According to results a dose of Ovaprim™ is recommended to be used the 0.25 μl/g body weight in the spermiation induction of silver rasbora., (Published by Elsevier Inc.)