1. IMPY, a potential beta-amyloid imaging probe for detection of prion deposits in scrapie-infected mice.
- Author
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Song PJ, Bernard S, Sarradin P, Vergote J, Barc C, Chalon S, Kung MP, Kung HF, and Guilloteau D
- Subjects
- Alzheimer Disease diagnostic imaging, Alzheimer Disease pathology, Animals, Antibodies, Monoclonal chemistry, Autoradiography, Binding, Competitive, Brain diagnostic imaging, Brain pathology, Disease Models, Animal, Humans, Iodine Radioisotopes pharmacokinetics, Mice, Plaque, Amyloid pathology, Radionuclide Imaging, Radiopharmaceuticals pharmacokinetics, Scrapie pathology, Amyloid beta-Peptides chemistry, Isotope Labeling methods, Plaque, Amyloid diagnostic imaging, Prions chemistry, Pyrazoles pharmacokinetics, Scrapie diagnostic imaging
- Abstract
Introduction: A potential single-photon emission computed tomography imaging agent for labeling of A beta plaques of Alzheimer's disease, IMPY (2-(4'-dimethylaminophenyl)-6-iodo-imidazo[1,2-a]pyridine), would be effective in detection of prion amyloid deposits in transmissible spongiform encephalopathies (TSEs)., Methods: In vitro autoradiographic studies were carried out with [125 I]IMPY on brain sections from scrapie-infected mice and age-matched controls. Competition study was performed to evaluate the prion deposit binding specificity with nonradioactive IMPY., Results: Binding of [125 I]IMPY was observed in infected brain sections, while on age-matched control brain sections, there was no or very low labeling. Prion deposit binding was confirmed by histoblots with prion protein-specific monoclonal antibody 2D6. In the presence of nonradioactive IMPY, the binding of [125 I]IMPY was significantly inhibited in all regions studied., Conclusions: These findings indicate that IMPY can detect the prion deposits in vitro in scrapie-infected mice. Labeled with 123 I, this ligand may be useful to quantitate prion deposit burdens in TSEs by in vivo imaging.
- Published
- 2008
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