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We examined the impact of varying ageing times (0, 6, 12, and 15 months) on the alterations in the concentration of key volatile compounds in Cheddar cheese as it ripened. With PCA of E-nose and PLS-DA of gas GC–MS and GC-IMS, the cheddar cheese samples could be identified from one another. As the cheese samples ripened longer, their overall acid, ester, and aldehyde concentrations increased. The important flavor biomarkers of ripened Cheddar cheese were 1-hexanol, 1-octen-3-ol, 2,6-dimethylpyrazine, octanal, decanal, 1-pentanol, butanoic acid ethyl ester, (E)-2-octenal, nonanoic acid, hexanal, dimethyltrisulfide, (E)-2-undecenal, and 1-heptanol, according to chemometric methods. The cheese samples that were ripened for 0 and 6 months showed a considerable contribution of the milky flavor. There was a strong negative association between the milky flavor and acetic acid. The aged Cheddar cheese exhibited distinct nutty, sour, and fruity flavors after 12 and 15 months. The fruity flavor was highly positively correlated with hexanal, octanal, and 2-heptanone, butanoic acid, ethyl ester, and acetic acid. The sour and nutty flavors were also strongly positively correlated with acids, while the sour and nutty flavors were positively influenced by ethyl hexanoate and benzaldehyde. This study provides data on the composition and variations in the flavor profile of ripening cheddar cheese.

Objectives: To evaluate the neutralizing antibody (NAb) levels against the SARS-CoV-2 Omicron variants BF.7, BQ.1, BQ.1.1, XBB.1, and XBB.1.5 after vaccination and natural infection. Methods: The NAbs against the different viral strains of 490 individuals with SARS-CoV-2 and 187 without SARS-CoV-2 in the Beijing COVID-19 outbreak during December 2022 to January 2023 were analyzed. Results: In uninfected individuals, limited levels of NAbs were produced against the prototype and variant strains after two doses vaccine but significantly increased after three or four doses of the vaccine. The infected individuals had high NAbs levels against the BF.7, BQ.1, and BQ.1.1 variants and moderate NAbs levels against the XBB.1 and XBB.1.5 variants. The highest NAbs levels were observed after two inoculation doses. The third and fourth doses vaccine did not result in a significant increase the NAbs levels. After the last dose of vaccination, the NAbs levels peaked at 12 months for the prototype and BF.7 and between 6 to 12 months for the BQ.1, BQ.1.1, XBB.1, and XBB.1.5 variants. Conclusions: The immune response decreases as the virus mutates. If booster vaccination is considered necessary, it is suggested for at least 6 months after infection.

Severe capacity degradation at high operating voltages and poor interphase stability at elevated temperature have thus far precluded the practical application of LiNi0.5Mn1.5O4 (LNMO) as a cathode material for lithium-ion batteries. Addressing these challenges through a combination of experimental and theoretical methods in this work, we demonstrate how a fluorinated carbonate electrolyte enables both high-voltage and high temperature operation by mitigating the traditional interfacial reactions observed in electrolytes with conventional carbonate solvents. Computational studies confirm the exceptional oxidation stability of fluorinated carbonate electrolyte which reduces deprotonation at high voltage. The mitigated deprotonation will then minimize the formation of HF acid which corrodes the LNMO surface and leads to phase transformation and poor interphases. With fluorinated carbonate electrolyte at elevated temperature, it was found on LNMO’s subsurface a reduced amount of Mn3O4 phase which can block Li+ transfer and result in drastic cell failure. Leveraging this approach, LNMO/graphite full cells with a high loading of 3.0 mAh/cm2 achieve excellent cycling stability, retaining ∼84 % of their initial capacity at room temperature (25 °C) after 200 cycles and ∼68 % after 100 cycles at 55 °C. This advanced electrolyte also shows promise for improving calendar life, retaining >30 % more capacity than the carbonate baseline after high temperature storage. These results indicate that electrolytes based on fluorinated carbonates are a promising strategy for overcoming the remaining challenges toward practical commercial application of LNMO.

Nonanal, (E)-2-nonenal, (E,E)-2,4-nonadienal, and (E,Z)-2,6-nonadienal were used to study the effect of number and position of the unsaturated bond in aliphatic aldehydes on meat flavorings. Cysteine-Amadori and thiazolidine derivatives were synthesized, identified by UPLC-TOF/MS and NMR, and quantitatively by UPLC-MS/MS. The polyunsaturated aldehydes exhibited higher inhibition than monounsaturated aldehydes, and monounsaturated aldehydes exhibited higher inhibition than saturated aldehydes, mainly manifested by the inhibition of the cysteine-Amadori formation and acceleration of the thiazolidine derivatives formation. The effect of unsaturated bonds position in aliphatic aldehydes on the initial Maillard reaction stage was similar. The cysteine played an important role in catalyzing the reaction of aliphatic aldehydes. A total of 109 volatile compounds derived by heating prepared thiazolidine derivatives degradation were detected by GC-MS. Formation pathways of volatile compounds were proposed by retro-aldol, oxidation, etc. Particularly, a route to form thiazole by the decarboxylation reaction of thiazolidine derivatives which derivatives from formaldehyde reacting with cysteine was proposed.

We characterize the suitability of two additive manufactured materials for use in shielding thermal neutrons for neutron scattering instrumentation. We compare two different boron based additive manufactured masks to a traditionally manufactured boron nitride mask in a real-use case scenario. The first additive manufactured mask is a composite of aluminum and boron carbide. The second additive manufactured mask is a composite of boron carbide and cyanoacrylate. We report the thermal conductivity of the aluminum boron carbide mask in comparison to other relevant materials. The aluminum boron carbide material may find use in shielding applications where thermal conductivity, a low total neutron scattering cross section, and low toxicity are criteria.

Objectives: To determine the status of immune responses after primary and booster immunization for SARS-CoV-2 variants and evaluate the differences in disease resistance based upon titers of neutralizing antibodies (NAbs) against the variants. Methods: Participants aged 18–59 years received 2 doses of inactivated COVID-19 vaccine, 14 days apart, and a booster dose after 12 months. Blood samples were collected before vaccination (baseline), 1 and 6 months after primary immunization, and at multiple instances within 21 days of the booster dose. NAbs against the spike protein of Wuhan-Hu-1 and 3 variants were measured using pseudovirus neutralization assays. Results: Of 400 enrolled participants, 387 completed visits scheduled within 6 months of the second dose and 346 participants received the booster dose in the follow-up research. After 1 month of primary immunization, geometric mean titers (GMTs) of NAbs peaked for Wuhan-Hu-1, whereas GMTs of other variants were

A molecular sensory science approach was used to explore the effects of ultrasonic treatment on aroma compounds of watermelon juice. Watermelon juice was submitted to ultrasonic power at 325 W for 20 min. Ultrasonic treatment reduced odor related to cucumber and green descriptors, whilst significantly improved odors related to sweet, floral, and fruity descriptors, thus contributing to the overall flavor of watermelon juice. Compared with untreated watermelon juice, the amount and concentration of volatile compounds in ultrasonicated watermelon juice increased by 82.50% and 111.84%, respectively. Notably, 22 alkene compounds were newly formed in ultrasonicated watermelon juice, which contributed to sweet and fruity aroma of watermelon juice. The findings of the present study suggest that ultrasonic treatment may be a potential method to improve the overall flavor of watermelon juice.

Composting is an effective and necessary modality in the recycling of agricultural wastes such as livestock manure, furfural, and straw. However, the risks of heavy metals (HMs) and antibiotic resistance genes (ARGs) during industrial-scale composting process have not been adequately assessed, especially with the addition of bacterial agents. In this study, changes in HMs toxicity, ARGs propagation and microbial community structure during industrial-scale aerobic composting of livestock manure were firstly investigated with various substrates addition. Moreover, the effect of the addition of bacterial agents (Bacillus strains) was evaluated. The results showed that industrial aerobic composting process could immobilize various HMs with different extents and significantly reduce the levels of ARGs such as intl1 and oqxB genes. The addition of Bacillus strains could further reduce the levels of most detected ARGs and the bioavailability of Cu and Cr, and the relative abundance of ereA and tetA was undetectable in some materials. After composting, the main bacterial community structures were similar among different substrates irrespective of bacterial agents and indicated that the composting process was the main driver for their change. This study provides a scientific reference for the safe reuse of livestock manure.

Our previous study indicated that intravenous vitamin C (IVC) treatment concurrent with modulated electrohyperthermia (mEHT) was safe and improved the quality of life (QoL) of non-small-cell lung cancer (NSCLC) patients. The aim of this trial was to further verify the efficacy of the above combination therapy in previously treated patients with refractory advanced (stage IIIb or IV) NSCLC. A total of 97 patients were randomized to receive IVC and mEHT plus best supportive care (BSC) (n = 49 in the active arm, receiving 1 g/kg * d IVC concurrently with mEHT, three times a week for 25 treatments in total) or BSC alone (n = 48 in the control arm). After a median follow-up of 24 months, progression-free survival (PFS) and overall survival (OS) were significantly prolonged by combination therapy compared to BSC alone (PFS: 3 months vs 1.85 months, P

Oral anticancer drugs have the advantages of convenient and flexible administration, however, they also face some new problems related to their oral preparation. Herein we describe a case of advanced non-small-cell lung cancer patient treated with gefitinib who had long-term adverse reactions of rashes and diarrhea, and his rashes disappeared after taking alkaline soda, and then reappeared after stopping drinking it. Imaging progress was also observed. To our knowledge, this is the first report on the effect of alkaline food on gefitinib-induced rashes dynamic change. In this case, the rash acted as a signal of therapeutic efficacy. Clinicians and pharmacists should be aware of potential and common factors that affect drug efficacy and strive to achieve the best therapeutic results.

The retention and elution of proteins in ion-exchange chromatography is routinely controlled by adjusting the mobile phase salt concentration. It has repeatedly been observed, as judged from adsorption isotherms, that the apparent heterogeneity of adsorption is lower at more-eluting, higher ionic strength. Here, we present an investigation into the mechanism of this phenomenon using a single-molecule, super-resolution imaging technique called motion-blur Points Accumulation for Imaging in Nanoscale Topography (mbPAINT). We observed that the number of functional adsorption sites was smaller at high ionic strength and that these sites had reduced desorption kinetic heterogeneity, and thus narrower predicted elution profiles, for the anion-exchange adsorption of α-lactalbumin on an agarose-supported, clustered-charge ligand stationary phase. Explanations for the narrowing of the functional population such as inter-protein interactions and protein or support structural changes were investigated through kinetic analysis, circular dichroism spectroscopy, and microscopy of agarose microbeads, respectively. The results suggest the reduction of heterogeneity is due to both electrostatic screening between the protein and ligand and tuning the steric availability within the agarose support. Overall, we have shown that single molecule spectroscopy can aid in understanding the influence of ionic strength on the population of functional adsorbent sites participating in the ion-exchange chromatographic separation of proteins., (Copyright © 2014 Elsevier B.V. All rights reserved.)