1. Ultrasensitive detection of alpha-synuclein oligomer using a PolyD-glucosamine/gold nanoparticle/carbon-based nanomaterials modified electrochemical immunosensor in human plasma
- Author
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Qiong Yuan, Dan Tao, Shizhen Song, Nicole Jaffrezic-Renault, Yingying Gu, Emily P. Nguyen, Jing Cheng, Hongzhi Pan, Zhenzhong Guo, Hubei Provincial Natural Science Foundation, and National Natural Science Foundation of China
- Subjects
Detection limit ,Graphene ,Chemistry ,010401 analytical chemistry ,Nanoparticle ,02 engineering and technology ,Carbon nanotube ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Oligomer ,0104 chemical sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Colloidal gold ,law ,0210 nano-technology ,Selectivity ,Spectroscopy ,Nuclear chemistry - Abstract
To discover the early stage Parkinson’s disease (PD) patients and provide treatment ideas, an ultrasensitive poly (D-glucosamine)/gold nanoparticles/ multi-walled carbon nanotubes/reduced graphene oxide (PDG/AuNPs/MWCNTs/rGO) modified immunosensor was proposed to detect alpha-synuclein oligomer (α-syno), which is the early biomarker in PD. As signal amplification materials, the PDG/AuNPs/ MWCNTs/rGO nanocomplex was immobilized on the glassy carbon electrode surface, and anti-α-syno antibody that specifically recognizes and binds α-syno is then grafted. The immunosensor revealed a linear response between 0.05 fM and 500.00 fM, using square wave voltammetry (SWV), and a detection limit of 0.03fM was found. The immunosensor with excellent sensitivity, selectivity and stability has been applied to the analysis of α-syno in human plasma samples., The authors gratefully acknowledge the financial supports from “ChuTian Scholar” Project Award of Hubei Province of China, the Open Foundation of Hubei Province Key Laboratory of Occupational Hazard Identification and Control (NO. OHIC2018G04) and the National Natural Science Foundation of China (No. 21676305 and No. 81973097).
- Published
- 2020