Search

Your search keyword '"Shi-De Lu"' showing total 2 results
Start Over Author "Shi-De Lu" Publisher elsevier
2 results on '"Shi-De Lu"'

1. Caspase inhibition attenuates accumulation of β-amyloid by reducing β-secretase production and activity in rat brains after stroke

Author

Man Xiong, Ting Zhang, Lin-Mei Zhang, Shi-De Lu, Ya-Lin Huang, and Feng-Yan Sun

Subjects
Brain ischemia, β-amyloid peptide, β-secretase, Caspase-3, Caspase-3 inhibitor, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

In this study, we tested if caspase-3 inhibition decreased ischemia-induced Aβ elevation by reducing β-secretase (BACE1) activity. Changes in caspase-3, Aβ and BACE1 levels were detected in rat striatum on different days after middle cerebral artery occlusion using immunostaining. We found that the positive labeled cells of activated caspase-3, Aβ, and BACE1 were significantly and time-dependently increased in the ipsilateral striatum. The results of Western blotting and RT-PCR showed that caspase-3 inhibitor Z-DEVD-FMK reduced BACE1 mRNA and protein levels, and inhibited its protease activity, thereby decreasing the amount of APP C99 and Aβ in ischemic brains. Moreover, Z-DEVD-FMK reduced BACE1 and GFAP double-labeled cells, but not GFAP protein levels or GFAP-labeled cells, in the ipsilateral striatum. Thus, we demonstrated that caspase-3 inhibition attenuated ischemia-induced Aβ formation by reducing BACE1 production and activity. This finding provides a therapeutic strategy for preventing Aβ accumulation and reducing the risk of neurodegeneration after stroke.

Published
2008
Full Text
View/download PDF

2. Caspase inhibition attenuates accumulation of β-amyloid by reducing β-secretase production and activity in rat brains after stroke

Author

Feng-Yan Sun, Man Xiong, Ting Zhang, Ya-Lin Huang, Shi-De Lu, and Lin-Mei Zhang

Subjects
Male, medicine.medical_specialty, β-amyloid peptide, Blotting, Western, Gene Expression, Nerve Tissue Proteins, Caspase 3, Striatum, lcsh:RC321-571, Rats, Sprague-Dawley, Brain ischemia, Internal medicine, mental disorders, medicine, Animals, Aspartic Acid Endopeptidases, β-secretase, Protease Inhibitors, RNA, Messenger, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Caspase, Analysis of Variance, Amyloid beta-Peptides, Microscopy, Confocal, biology, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Neurodegeneration, Infarction, Middle Cerebral Artery, medicine.disease, Caspase Inhibitors, Immunohistochemistry, Corpus Striatum, Rats, Blot, Endocrinology, Neurology, Caspase-3, Caspase-3 inhibitor, biology.protein, Amyloid Precursor Protein Secretases, business, Oligopeptides, Amyloid precursor protein secretase, Neuroscience, Immunostaining
Abstract

In this study, we tested if caspase-3 inhibition decreased ischemia-induced Abeta elevation by reducing beta-secretase (BACE1) activity. Changes in caspase-3, Abeta and BACE1 levels were detected in rat striatum on different days after middle cerebral artery occlusion using immunostaining. We found that the positive labeled cells of activated caspase-3, Abeta, and BACE1 were significantly and time-dependently increased in the ipsilateral striatum. The results of Western blotting and RT-PCR showed that caspase-3 inhibitor Z-DEVD-FMK reduced BACE1 mRNA and protein levels, and inhibited its protease activity, thereby decreasing the amount of APP C99 and Abeta in ischemic brains. Moreover, Z-DEVD-FMK reduced BACE1 and GFAP double-labeled cells, but not GFAP protein levels or GFAP-labeled cells, in the ipsilateral striatum. Thus, we demonstrated that caspase-3 inhibition attenuated ischemia-induced Abeta formation by reducing BACE1 production and activity. This finding provides a therapeutic strategy for preventing Abeta accumulation and reducing the risk of neurodegeneration after stroke.

Published
2008

Catalog

Books, media, physical & digital resources
See catalog results