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Background: In areas co-endemic for Plasmodium vivax and Plasmodium falciparum there is an increased risk of P vivax parasitaemia following P falciparum malaria. Radical cure is currently only recommended for patients presenting with P vivax malaria. Expanding the indication for radical cure to patients presenting with P falciparum malaria could reduce their risk of subsequent P vivax parasitaemia., Methods: We did a multicentre, open-label, superiority randomised controlled trial in five health clinics in Bangladesh, Indonesia, and Ethiopia. In Bangladesh and Indonesia, patients were excluded if they were younger than 1 year, whereas in Ethiopia patients were excluded if they were younger than 18 years. Patients with uncomplicated P falciparum monoinfection who had fever or a history of fever in the 48 h preceding clinic visit were eligible for enrolment and were required to have a glucose-6-dehydrogenase (G6PD) activity of 70% or greater. Patients received blood schizontocidal treatment (artemether-lumefantrine in Ethiopia and Bangladesh and dihydroartemisinin-piperaquine in Indonesia) and were randomly assigned (1:1) to receive either high-dose short-course oral primaquine (intervention arm; total dose 7 mg/kg over 7 days) or standard care (standard care arm; single dose oral primaquine of 0·25 mg/kg). Random assignment was done by an independent statistician in blocks of eight by use of sealed envelopes. All randomly assigned and eligible patients were included in the primary and safety analyses. The per-protocol analysis excluded those who did not complete treatment or had substantial protocol violations. The primary endpoint was the incidence risk of P vivax parasitaemia on day 63. This trial is registered at ClinicalTrials.gov, NCT03916003., Findings: Between Aug 18, 2019, and March 14, 2022, a total of 500 patients were enrolled and randomly assigned, and 495 eligible patients were included in the intention-to-treat analysis (246 intervention and 249 control). The incidence risk of P vivax parasitaemia at day 63 was 11·0% (95% CI 7·5-15·9) in the standard care arm compared with 2·5% (1·0-5·9) in the intervention arm (hazard ratio 0·20, 95% CI 0·08-0·51; p=0·0009). The effect size differed with blood schizontocidal treatment and site. Routine symptom reporting on day 2 and day 7 were similar between groups. In the first 42 days, there were a total of four primaquine-related adverse events reported in the standard care arm and 26 in the intervention arm; 132 (92%) of all 143 adverse events were mild. There were two serious adverse events in the intervention arm, which were considered unrelated to the study drug. None of the patients developed severe anaemia (defined as haemoglobin <5 g/dL)., Interpretation: In patients with a G6PD activity of 70% or greater, high-dose short-course primaquine was safe and relatively well tolerated and reduced the risk of subsequent P vivax parasitaemia within 63 days by five fold. Universal radical cure therefore potentially offers substantial clinical, public health, and operational benefits, but these benefits will vary with endemic setting., Funding: Australian Academy of Science Regional Collaborations Program, Bill & Melinda Gates Foundation, and National Health and Medical Research Council., Competing Interests: Declaration of interests KT is funded by a CSL Century fellowship. JAS and RNP are funded by National Health and Medical Research Council Leadership Investigator Grants (1196068 and 2008501). All other authors declare no competing interests., (Copyright © 2023 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved.)

Ivermectin, a US Food and Drug Administration-approved anti-parasitic agent, was found to inhibit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication in vitro. A randomized, double-blind, placebo-controlled trial was conducted to determine the rapidity of viral clearance and safety of ivermectin among adult SARS-CoV-2 patients. The trial included 72 hospitalized patients in Dhaka, Bangladesh, who were assigned to one of three groups: oral ivermectin alone (12 mg once daily for 5 days), oral ivermectin in combination with doxycycline (12 mg ivermectin single dose and 200 mg doxycycline on day 1, followed by 100 mg every 12 h for the next 4 days), and a placebo control group. Clinical symptoms of fever, cough, and sore throat were comparable among the three groups. Virological clearance was earlier in the 5-day ivermectin treatment arm when compared to the placebo group (9.7 days vs 12.7 days; p = 0.02), but this was not the case for the ivermectin + doxycycline arm (11.5 days; p = 0.27). There were no severe adverse drug events recorded in the study. A 5-day course of ivermectin was found to be safe and effective in treating adult patients with mild COVID-19. Larger trials will be needed to confirm these preliminary findings., (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Ultrafast Laplace NMR (UF-LNMR) reduces the experiment time of multidimensional relaxation and diffusion measurements to a fraction. Here, we demonstrate a method for nonlinear (in this case logarithmic) sampling of the indirect dimension in UF-LNMR measurements. The method is based on the use of frequency-swept pulses with the frequency nonlinearly increasing with time. This leads to an optimized detection of exponential experimental data and significantly improved resolution of LNMR parameters., (Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.)

Problematic Internet Use (PIU) has become a concern for public mental health across the globe. However, there are few studies assessing PIU in Bangladesh. The present cross-sectional study estimated the prevalence rate of PIU and its associated risk factors among 405 university students in Bangladesh between June and July 2018. The measures included sociodemographic questions, internet and health-related variables, the Internet Addiction Test (IAT) and the Depression, Anxiety and Stress Scale (DASS-21). The prevalence of PIU was 32.6% among the respondents (cut-off score of ≥50 on the IAT). The prevalence of PIU was higher in males in comparison to females, although the difference was not statistically significant. Internet-related variables and psychiatric comorbidities were positively associated with PIU. From the unadjusted model, more frequent use of the internet and more time spent on the internet were identified as strong predictors of PIU, whereas the adjusted model showed depressive symptoms and stress only as strong predictors of PIU. It is hoped this preliminary study will facilitate further study on PIU along with other psychiatric disorders in Bangladesh., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Safety behaviors in pesticide use are considered the most important determinants of the adverse health effects among farmers. In addition, the risk of pesticide exposure is strongly associated with farmers' behavior when working with pesticides. However, there is limited understanding of farmers' safety behavior and its determinants, particularly in developing countries like Iran. A field survey of 373 rice producers was conducted in Mazandaran Province of northern Iran to study determinants of farmers' safety behavior when working with pesticides. Although farmers' safety behavior varied depending on the type of safety measures adopted, few farmers showed safe behaviors in using personal protective equipment (PPE) (8.9%), in following appropriate practices in pesticide use (8.6%), in following hygiene practices after using pesticides (2.7%), and in avoiding health risks (2.4%). The mean scores of importance of all four components of safety behavior were higher than the mean scores of their current use, implying that perceptions of importance of various types of safety measures during pesticide application were not fully reflected in farmers' practices. Perceived barriers of using safety measures were the most reliable predictor of farmers' safety behavior in pesticide use. Perceived barriers of using safety measures and mean number of pesticide applications per cropping season had a negative impact on farmers' safety behaviors. By contrast, the variables education, level of self-consumed production in the household, experience of health risks related to working with pesticides, regular health check-ups, and perception of pesticide hazards promoted farmers' safety behaviors. The study not only presents a clear framework for better understanding and assessing farmers' safety behavior in pesticide use, but also provides valuable inputs for the design of effective interventions that could support farmers in the implementation of safety measures in Iran., (Copyright © 2018. Published by Elsevier B.V.)

Personal protective equipment (PPE) can substantially reduce the risk exposure from pesticide spraying, but compliance is rather low, particularly among small-scale farmers. In this study the connection between farmers' willingness to use PPE in pesticide handling and perceptions of PPE usefulness was examined through a survey of 341 small-scale farmers in Gorgan county of Golestan Province, Iran. Farmers who were not using PPE when working with pesticides were purposively selected to better serve the objective of the study. Multinomial logit regression models were employed to examine differences in farmers' willingness to use PPE in the future. Almost four out of ten farmers (38.1%) showed willingness to use PPE. However, 36.7% of the farmers showed unwillingness and 25.2% were unsure about using PPE. The average score of farmers' perceptions of PPE disadvantages (x=0.55) was significantly higher than the corresponding score of PPE advantages (x=0.38). Low availability and high price were considered the most important constraints in PPE use by the majority of farmers (75.4% and 74.8% of the farmers, respectively). Farmers who perceived usefulness of PPE, such as effectiveness, safety, and ease of use, were more willing to use PPE in the future. Those who perceived non-usefulness of PPE, driven by unavailability, high price, lack of use by neighbors or colleagues, and ignorance of PPE in extension trainings, were less likely to use PPE in the future. Findings revealed that willingness to use PPE among small-scale farmers when working with pesticides is strongly linked to their perception of PPE usefulness. Findings raise our understanding of the important role of farmers' knowledge in PPE acceptance and use and can motivate policy-makers to pay more attention to the role of farmers' perceptions and awareness in the success or failure of health and safety programs. It is necessary to incorporate farmers' preferences in extension programs to promote safety measures during working with pesticides., (Copyright © 2017 Elsevier B.V. All rights reserved.)

In this work a simple, inexpensive, and sensitive optical sensor based on triacetylcellulose membrane as solid support was developed by using immobilization of Giemsa indicator for pH measurement. In this method, the influence variables on the membrane performance including pH concentration of indicator, response time, ionic strength, and reversibility were investigated. At optimum values of all variables the response of optical pH sensor is linear in the pH range of 3.0-12.0. This optical sensor was produced through simultaneous binding of the Giemsa on the activated triacetylcellulose membrane which responded to the pH changes in a broader linear range within less than 2.0 min and suitable reproducibility (RSD<5%). Stability results showed that this sensor was stable after 6 months of storage in the water/ethanol (50:50, v/v) solution without any measurable divergence in response properties (less than 5% RSD)., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Most lymphomas show an increased incidence and poorer prognosis in males vs females, suggesting endocrine regulation. We have previously shown that tumor growth in vivo of a murine T-cell-derived lymphoma is repressed following activation of estrogen receptor β (ERβ, ESR2). By using ERβ-deficient mice, we now demonstrate that this inhibition is mediated via a direct effect on the tumor cells and not on the microenvironment. Furthermore, we show that the growth-suppressing effects of ERβ agonist are also valid for human B-cell lymphomas as demonstrated in tumors derived from Granta-519 mantle cell lymphoma (MCL) and Raji Burkitt lymphoma (BL) cells. In Granta-519 MCL tumors, activation of ERβ reduced expression of BAFF and GRB7, 2 important molecules involved in B-cell proliferation and survival. Importantly, activation of ERβ inhibited angiogenesis and lymphangiogenesis, possibly mediated by impaired vascular endothelial growth factor C expression. Furthermore, using disseminating Raji BL cells, we show that ERβ activation reduces dissemination of grafted Raji BL tumors. We also show by immunohistochemistry that ERβ is expressed in primary MCL tissue. These results suggest that targeting ERβ with agonists may be valuable in the treatment of some lymphomas, affecting several aspects of the malignant process, including proliferation, vascularization, and dissemination.