1. Magnetic hydrogels for levodopa release and cell stimulation triggered by external magnetic field.
- Author
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Kondaveeti S, Semeano ATS, Cornejo DR, Ulrich H, and Petri DFS
- Subjects
- Alginates chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Dopamine Agents administration & dosage, Dopamine Agents chemistry, Dopamine Agents pharmacokinetics, Drug Delivery Systems methods, Drug Liberation, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Hydrogen-Ion Concentration, Levodopa administration & dosage, Levodopa pharmacokinetics, Microscopy, Electron, Scanning, Polysaccharides, Bacterial chemistry, Hydrogels chemistry, Levodopa chemistry, Magnetic Fields, Magnetics
- Abstract
Magnetic responsive hydrogels composed of alginate (Alg) and xanthan gum (XG), crosslinked with Ca
2+ ions, were modified by in situ magnetic nanoparticles (MNP) formation. In comparison to magnetic Alg hydrogels, magnetic Alg-XG hydrogels presented superior mechanical and swelling properties, due to the high charge density and molecular weight of XG. The loading efficiency of levodopa (LD), an important antiparkinson drug, in the Alg-XG/MNP hydrogels was the highest (64%), followed by Alg/MNP (56%), Alg-XG (53%) and Alg (28%). A static external magnetic field (EMF) of 0.4 T stimulated the release of LD from Alg-XG/MNP hydrogels achieving 64 ± 6% of the initial loading after 30 h. The viability, proliferation and expression of dopaminergic markers of human neuroblastoma SH-SY5Y cell on the LD loaded magnetic hydrogels were successful, particularly under EMF, which stimulated the release of LD. Overall, the results of this study provided the rational design of magnetic hydrogels for the delivery of drugs, which combined with external magnetic stimulus, might improve cell proliferation and specific differentiation., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
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