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Your search keyword '"Ryutaro Fukumura"' showing total 3 results
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3 results on '"Ryutaro Fukumura"'

1. List of Contributors

Author

Bernhard Aigner, W. Ted Allison, Roberto Andreatini, Marta Antonelli, Saskia S. Arndt, Amazon Austin, Cristin Brand, Joanna Bukowska, Andrew C. Caprariello, Rachel E. Carlisle, Lucas M. Chaible, Li Chen, Mengqi Chen, Thomas Chi, Mohammed E. Choudhury, Iain J. Clarke, Kristine Coleman, Gabriela D. Colpo, Oliver S. Damm, Catherine M. Davis, Erica E. Davis, Luis de Lecea, Aline S. de Miranda, Diogo B. de Souza, Jeffrey G. Dickhout, Paul J. Donaldson, Geoffrey A. Donnan, Yuko Emoto, David Feifel, Marcus A. Finzi Corat, Martin G. Frasch, Ryutaro Fukumura, Toshio Fukusato, John-Paul Fuller-Jackson, Sharyl Fyffe-Maricich, Barbara Gawronska-Kozak, Godfrey S. Getz, Yoichi Gondo, Belinda A. Henry, David W. Howells, Sumit Jamwal, Jiro Kasahara, Nicholas Katsanis, Matthew W. Kemp, Barbara Kessler, Imran Khan, Aileen King, Denise Kinoshita, Nikolai Klymiuk, Ryuta Koyama, Doris Kretzschmar, Puneet Kumar, Jitka Kuncova, Maki Kuro, Mayuko Kurome, Michael Lardelli, Heather A. Lawson, Lars Lewejohann, Julie C. Lim, David M. Lyons, Malcolm R. Macleod, Shigeru Makino, Marta Martinez-Lage, Renita M. Martis, Ellen Meijer, Gerlinde A.S. Metz, Robert H. Miller, Yuichiro Miura, Zahraa Mohammed-Ali, Gabrielle C. Musk, Samera Nademi, Aysegul Nalca, Morgan Newman, Yu Nie, Noriko Nishikawa, Masahiro Nomoto, Rebecca E. Nordquist, Victoria E. O’Collins, Masatoshi Ogawa, Marco A. Pereira-Sampaio, Jean-Paul Praud, João Quevedo, Catherine A. Reardon, Christopher P. Regan, Simone Renner, Fabiola M. Ribeiro, Natalia P. Rocha, Sandra Rodriguez-Perales, Sara I. Ruiz, Masatoshi Saito, Francisco J.B. Sampaio, Steven J. Schapiro, Alan F. Schatzberg, Jay Schulkin, Angela L. Shamchuk, Richard P. Shannon, You-Tang Shen, Paul D. Shilling, Milan Stengl, Marshall L. Stoller, Stephen R. Stürzenbaum, Jitka Sviglerova, Kazumi Taguchi, Yoshihisa Takahashi, Akie Tanabe, Junya Tanaka, Jerrold Tannenbaum, Antônio L. Teixeira, Jeffrey M. Testani, Keith B. Tierney, Raul Torres-Ruiz, Airo Tsubura, Ryosuke Tsuji, David T. Tzou, Haruo Usuda, Samira S. Valvassori, Franz J. van der Staay, Roger B. Varela, Giuseppe Verdile, Irene Vorontsova, Jessica P. Wayhart, James L. Weed, JoEllen Welsh, Steffi Werler, Joachim Wistuba, Eckhard Wolf, Annegret Wünsch, Toshiyuki Yamamoto, Hironori Yokoyama, Katsuhiko Yoshizawa, Maria L. Zaidan Dagli, Jennifer Y. Zhang, Yu Zhou, and Elizabeth E. Zumbrun

Published
2017
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2. Forward and Reverse Genetics to Model Human Diseases in the Mouse

Author

Yoichi Gondo, Ryutaro Fukumura, and Shigeru Makino

Subjects
Genetics, symbols.namesake, Genome editing, Mendelian inheritance, symbols, Gene targeting, Genomics, Identification (biology), Computational biology, Biology, Functional genomics, Genome, Reverse genetics
Abstract

To establish human disease models in the mouse, various forward and reverse genetics tools have been developed. New technologies, for example, high-throughput identification of novel mutations, gene targeting, and genome editing, in the mouse genome have made the forward and reverse genetics possible. Worldwide efforts including a variety of international consortiums have been producing many mutant mice with useful annotations for functional genomics to elucidate the mechanisms behind diseases. The resource centers and public databases have also been built to enhance their utilities. The modeling has started from Mendelian monogenic traits and has been expanding to more complex quantitative traits as well. Multidisciplinary integration among, for example, molecular, cellular and developmental biology, genetics, genomics, medicine, statistics, and informatics must be orchestrated to fully utilized the resources and knowledge of model mice.

Published
2017
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3. ENU-induced mutant mice for a next-generation gene-targeting system

Author

Ryutaro Fukumura and Yoichi Gondo

Subjects
Genetics, Mutation, Mutant, Mutagenesis (molecular biology technique), Gene targeting, Biology, medicine.disease_cause, Genome, Molecular biology, Reverse genetics, genomic DNA, otorhinolaryngologic diseases, medicine, Gene
Abstract

By the N -ethyl- N -nitrosourea (ENU)-based gene-driven mutagenesis, it is now possible to obtain allelic series of mutant mouse strains, each of which carries a different base substitution in any target gene. This new reverse genetic tool has become available based on the ENU mutant mouse library. The ENU mutant mouse library consists of dual archives of frozen sperm and corresponding genomic DNA derived from Generation-1 (G1) male mice, each of which carries thousands of ENU-induced base substitutions. Firstly, ENU-induced mutations in the target gene are screened from the genomic DNA archive by using one of the high-throughput mutation discovery systems. The identified mutations are then revived as live mice by the in vitro fertilization (IVF) and embryo transfer (ET) technology. Just like the knockout (KO) mouse system, the revived mutant strains are finally subjected to the three-generation scheme to reveal the gene function(s) of the target gene. This new reverse genetics or “next-generation gene-targeting system” allows us to elucidate the biological roles of the mouse genome in terms of single base-pair effects not only for the protein-coding sequences but also for any genomic sequences.

Published
2009
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