1. Diversity and distribution of methane-oxidizing microbial communities associated with different faunal assemblages in a giant pockmark of the Gabon continental margin
- Author
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Marie-Anne Cambon-Bonavita, Antje Boetius, Myriam Sibuet, Sébastien Duperron, Eloise Delage, Jean-Claude Caprais, Thierry Nadalig, Erwan Roussel, Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire Environnement Profond (LEP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Max Planck Institute for Marine Microbiology, and Max-Planck-Gesellschaft
- Subjects
010504 meteorology & atmospheric sciences ,Microbial phylogeny ,Oceanography ,01 natural sciences ,Cold seep ,Vesicomyidae ,03 medical and health sciences ,AOM ,14. Life underwater ,0105 earth and related environmental sciences ,Chemosynthesis ,0303 health sciences ,biology ,030306 microbiology ,Ecology ,Pockmark ,Verrucomicrobia ,Faunal assemblage ,biology.organism_classification ,QR ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Cytophaga ,Chemosynthetic ecosystems ,Proteobacteria ,REGAB ,Archaea - Abstract
En libre-accès sur Archimer : http://archimer.ifremer.fr/doc/2009/publication-7298.pdf; International audience; A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Siboglinidae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5 cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups were detected in the pockmark sediments, as well as from both known bacterial partners. The archaeal diversity was limited to the ANME cluster for all investigated samples. The bacterial diversity included members of the Proteobacteria, Bacilliales, Cytophaga/Flavobacteria, Verrucomicrobia, JS1 and Actinobacteria clusters. Bacterial 16S rRNA gene sequences related to those of known sulphide-oxidizing symbionts were recovered from tissues of several invertebrates including vesicomyid clams and siboglinid tubeworms of REGAB.
- Published
- 2009
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