1. Proteomics approach in horticultural crops for abiotic-stress tolerance
- Author
-
Rakesh Kumar Sinha and Shiv Verma
- Subjects
Abiotic component ,Abiotic stress ,Quantitative proteomics ,Horticultural crops ,Identification (biology) ,Computational biology ,Biology ,Proteomics ,Cellular localization ,Isobaric tag for relative and absolute quantitation - Abstract
Abiotic stresses, for example, water stress, salinity, chilling, high temperature, and nutrient imbalance may influence the molecular and physiological process of plants in declining crop production. The molecular and physiological changes that hinder crop growth are foremost concerned since it affects directly meeting the global food demand. Abiotic stress reveals a profound impact on plant proteins, including alteration in posttranscriptional, cellular localization, and at the posttranslational levels. Understanding and identifying the role of differentially expressed protein during abiotic stress will be comparatively easier to deal with plant signaling to stresses. A proteomics approach like identification of abiotic stress–specific biomarkers, study of the selected protein accumulation pattern in response to different stresses, and elucidation of functional protein pathways is most important. Currently, gel electrophoresis, in addition to high throughput mass spectrometry (LC-MS-MS, MALDI-TOF) coupled with bioinformatics, is a widely used proteomics approach in plant-protein identification. This chapter focuses on advanced quantitative proteomics approaches like isobaric tag for relative and absolute quantitation (Iraq) and inductively coupled plasma-mass spectrometry approaches used to find novel and differentially expressed proteins. The proteomics approach and findings will enhance our understanding of the cellular processes and the pathways associated with abiotic-stress responses to develop and improve important agronomical traits.
- Published
- 2021