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Cholic acid based biodegradable reverse polymeric micelles have been widely utilized as preclinically suitable drug delivery system for poorly water-soluble drugs. In this report, we developed N-acetylcysteine functionalized cholic acid based triarmed poly (Dl-lactide) (ACyCA-triarmed (DLL) n as reversed polymeric micelles for drug delivery studies. ACyCA was synthesized via thiol-yne click reaction and subsequently used as an initiator for the synthesis of ACyCA-triarmed (DLL) n through ring opening polymerization (ROP) using Sn (Oct) 2 as a catalyst. The synthesized ACyCA-triarmed (DLL) n was characterized using GPC, FT-IR, 1 H NMR, 13 C NMR, spectrofluorometer, HR-TEM, DSC, TGA, XRD, DLS, and zeta potential techniques. The reverse critical micellar concentration of the polymer was determined to be 1.99 mg/mL using a spectrofluorometer. The synthesized reverse micelles (RMs) were utilized as a reducing and capping agent for the preparation of AuNPs under sunlight exposure. The formed AuNPs exhibited spherical in shape with an average size of ∼ 23.4 nm and D h was found to be 86.8 ± 1.3 nm as evidenced by the TEM and DLS analysis. Furthermore, the antimicrobial activity, hemolytic activity, anti-oxidant activity, and in-vitro drug release studies were examined for the RMs-AuNPs and compared with RMs. The hydrophobic nature of RMs and RMs-AuNPs had better haemocompatibility at above the reversed CMC. The antioxidant activity RMs-AuNPs showed better inhibitory effect in a dose-dependent manner as compared to RMs. The RMs-AuNPs formulation act as reservoir for solubilization of hydrophobic doxorubicin (Dox.HCl) drugs and can be used as therapeutic platform for slow and sustained release of drugs in biological medium., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Biosurfactant based biostimulants plays a vital role in agriculture filed by enhancing the soil quality, promote plant growth, and eliminate plant pathogens, and increasing nutrient uptake. This manuscript describes the synthesis of trimesic based lithocholic ester functionalized amphiphiles (TMLCEA) with oppositely charged head groups using thiol-yne click chemistry, which is an effective and simple approach. The trimesic based lithocholic ester functionalized zwitterionic penicillamine (TMLCEPA), cationic cysteamine·HCl (TMLCECy), and anionic thiomalic acid (TMLCETM) exhibited hierarchically self-assembled microstructures from below to above the CMC. In below the CMC, TMLCEPA, TMLCECy, and TMLCETM showed a bundle of petals, flower-like morphology, and grass seed-like patterns respectively. The morphology of self-assembly was studied by FE-SEM, DLS, OPM, contact angle, and zeta potential measurements. Among these amphiphiles, TMLCECy exhibited potential antimicrobial activity at above the CMC. The biostimulant effect of different concentration of TMLCEA treated with maize and green gram seeds were evaluated under in vitro condition, wherein TMLCECy showed improved seed germination and seedling parameters at 750 µL/mL as compared to TMLCEPA, TMLCETM and untreated amphiphiles as control. Molecular docking and molecular dynamic simulations show that TMLCEPA and TMLCETM showed higher binding affinity for dengue methyltransferase protein. The result of the present study opens up new avenues for bile acid-based amphiphiles as bio-based and cost-effective biostimulants for sustainable agriculture., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The use of marine waste derived chitosan (CS) for the synthesis of nanomaterials is considered as one of the effective routes for bio-waste management and recovering functional products. Herein, CS capped silver nanoparticles (Ag NPs-CS) with potential anticancer and dye pollutants adoption properties have been synthesized photochemically under direct sunlight. To obtain, CS, shrimp shell waste was subjected to a serious of standard demineralization, deproteinization and deacetylation processes. The electronic absorption peak (400 nm) denoting surface plasmonic resonance of Ag NPs and infrared peaks relevant to CS (3364 cm -1 of OH/NH 2 , 2932 cm -1 of CH, and 1647 cm -1 of -CO) exhibited peaks confirmed the formation of CS-Ag NPs. Ag NPs-CS exhibited anticancer activity against Human lung adenocarcinoma cell lines (A549), the maximum cell death noticed at the concentration of 20 μg/mL and 70 μg/mL was 20 and 52 %, respectively. An aqueous Ag NPs-CS (100 μg/mL) was degraded ≥95 % of mixed dye target solution (25 mg/mL) containing equal volume of cationic dye (Methylene blue and Rhodamine B) and anionic dye (methyl orange). Therefore, these findings suggest that the shrimp shell waste derived CS can be used for the synthesis of CS-Ag NPs with potential biomedical and environmental applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

A nanohybrid prepared from the lignocellulosic residue is a feasible approach to synthesize blue light emitting fluorescent doped TiO 2 quantum dot nanocomposite (C-TiO 2 QDs) by microwave techniques using Mandarin orange (Citrus reticulata) peel powder with titanium isopropoxide precursors. With a greater orange peel colloidal medium, the structure of the TiO 2 -NPs changed from a mixture of rutile and anatase phases to exclusively the anatase phase. The optical and morphological properties of as-prepared C-TiO 2 QDs were characterized by HR-TEM, XRD, FT-IR, UV-visible, PL spectra, DLS, and Zeta potential techniques. The reaction condition was optimized by changing substrate composition, pH, and reaction time. C-TiO 2 QDs exhibit outstanding stability at pH 7 and remain sustained for at least 180 days without aggregation. As prepared C-TiO 2 QDs have distinct emission and excitation activities with an average particle size of 2.8 nm. Cell viability was performed on normal L929 cells, where it showed excellent biocompatibility (<90 %) even at the concentration of 200 μg/mL after 24 h treatment. Additionally, the synthesized C-TiO 2 QDs were used with L929 cells as a fluorescent probe for bio-imaging applications. The results revealed that neither of the cell lines' morphologies had significantly changed, proving the biocompatibility of the synthetic C-TiO 2 QDs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)