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Objective: To assess the feasibility of an ultrasound (US)-guided quadratus lumborum (QL)-psoas muscle (Pm) interfascial plane injection using a dorsoventral (DV) approach, and to compare needle visualization and distribution pattern between DV and ventrodorsal (VD) approaches., Study Design: Experimental anatomical study., Animals: A total of 14 cat cadavers., Methods: Bilateral in-plane US-guided injections in the QL-Pm plane were performed at the level of the second lumbar (L2) transverse process. A spinal needle was inserted using either a VD or DV approach. A total volume of 0.4 mL kg -1 of a solution of ropivacaine 0.18%, tissue dye and iohexol (350 mg mL -1 ), in a proportion of 3:1:1, respectively, was injected bilaterally. Spread of injectate was compared and evaluated using US, computed tomography (CT) scanning and anatomical dissection. Presence of dye on the sympathetic trunk was compared between methods using Kappa (κ) coefficient of agreement., Results: The QL-Pm plane was visualized, and dye was present in the target plane using both approaches. Needle visualization in the VD approach was enhanced compared with the DV approach (p = 0.0005). Mean distribution along the sympathetic trunk was 4.8 and 4.1 segments in the VD and DV approaches, respectively, showing non-statistical differences. The ventral branches of L1, L2 and L3 were successfully stained in 11/14 and 6/14 using the VD and DV approaches, respectively. Substantial agreement (κ = 0.61) of sympathetic trunk staining was observed between CT scan and anatomical dissection. Dye was observed cranial to the diaphragm in 7/8 cases on CT with both approaches. Epidural and abdominal cavity dye distribution was not observed., Conclusions and Clinical Relevance: The DV approach to the QL-Pm plane could be an alternative to the VD approach. Both approaches seem capable of reaching the spinal nerves, the sympathetic trunk and both splanchnic and coeliac nerves in feline cadavers., (Copyright © 2022 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.)

Objectives: To describe the ultrasonographic (US) anatomy of the sublumbar region at the level of second lumbar vertebra (L2), to assess the feasibility of US-guided quadratus lumborum (QL) injections and to evaluate the dye distribution pattern in feline cadavers., Study Design: Descriptive feline cadaver study., Animals: A total of eight cat cadavers., Methods: Using a linear probe (6-14 MHz), with the cadavers in lateral recumbency, the QL and psoas muscles (Pm) were identified at the level of L2 and landmarks recorded. Using an in-plane technique, a spinal needle was inserted in a ventrodorsal direction to reach the interfascial plane between the QL and Pm. Using a ropivacaine, dye and iohexol solution, a total volume of 0.4 mL kg -1 was injected. Computed tomography (CT) scanning and anatomic dissection were performed to evaluate the spread of injectate. Presence of dye on the sympathetic trunk was compared between assessment methods using kappa coefficient of agreement (κ)., Results: Using US guidance, the QL-Pm interfascial plane was identified and dye solution was present in the target fascial plane in all animals. Injectate was distributed on the ventral roots of the spinal nerves between the first and third lumbar vertebrae in 6/8 cats, and on the sympathetic trunk from the thirteenth thoracic to the third lumbar vertebrae in 7/8. Dye was found on the major splanchnic nerve in 7/8 cats and on the minor splanchnic nerve and coeliac ganglion pathways in all animals. Agreement between dissection and CT images dye distribution on the sympathetic trunk was κ = 0.72., Conclusions and Clinical Relevance: The US-guided QL injection was feasible and injectate was present in the QL-Pm interfascial plane in all feline cadavers. In vivo studies are warranted to evaluate the analgesic efficacy of this technique for abdominal surgical procedures., (Copyright © 2021 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.)

Background: Plant defensins were discovered at beginning of the 90s'; however, their precise mechanism of action is still unknown. Herein, we studied ApDef 1 -Saccharomyces cerevisiae interaction., Methods: ApDef 1 -S. cerevisiae interaction was studied by determining the MIC, viability and death kinetic assays. Viability assay was repeated with hydroxyurea synchronized-yeast and pretreated with CCCP. Plasma membrane permeabilization, ROS induction, chromatin condensation, and caspase activation analyses were assessed through Sytox green, DAB, DAPI and FITC-VAD-FMK, respectively. Viability assay was done in presence of ascorbic acid and Z-VAD-FMK. Ultrastructural analysis was done by electron microscopy., Results: ApDef 1 caused S. cerevisiae cell death and MIC was 7.8μM. Whole cell population died after 18h of ApDef 1 interaction. After 3h, 98.76% of synchronized cell population died. Pretreatment with CCCP protected yeast from ApDef 1 induced death. ApDef 1 -S. cerevisiae interaction resulted in membrane permeabilization, H 2 O 2 increased production, chromatin condensation and caspase activation. Ascorbic acid prevented yeast cell death and membrane permeabilization. Z-VAD-FMK prevented yeast cell death., Conclusions: ApDef 1 -S. cerevisiae interaction caused cell death through cell cycle dependentprocess which requires preserved membrane potential. After interaction, yeast went through uncontrolled ROS production and accumulation, which led to plasma membrane permeabilization, chromatin condensation and, ultimately, cell death by activation of caspase-dependent apoptosis via., General Significance: We show novel requirements for the interaction between plant defensin and fungi cells, i.e. cell cycle phase and membrane potential, and we indicate that membrane permeabilization is probably caused by ROS and therefore, it would be an indirect event of the ApDef 1 -S. cerevisiae interaction., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Leishmaniosis caused by Leishmania infantum is a zoonotic disease endemic in many countries of America, Asia and Europe, including Portugal. Dogs are the major reservoir of L. infantum, but domestic cats may also be infected. Three clinical cases of feline leishmaniosis are described, with ocular clinical signs as the only manifestation of the disease. A case had bilateral anterior uveitis and a granulomatous conjunctivitis, another one presented keratitis and the third case had a nodular blepharitis. All the affected cats had high serum titres of antibodies to L. infantum, while polymerase chain reaction results were positive in two of the cats. Although all cats in the present study improved after treatment with meglumine antimoniate and/or allopurinol, one of them died 6months later apparently due to a systemic L. infantum infection. The prevalence of disease may be underestimated in cats, because leishmaniosis is often not considered in the differential diagnosis of feline diseases. Feline leishmaniosis should be suspected in cats with ocular clinical signs and in those living in or traveling to areas where the zoonosis is endemic., (Copyright © 2016 Elsevier Inc. All rights reserved.)