23 results on '"Pellegrino, S."'
Search Results
2. Phenotype and clinical characteristics of early compared to late-onset pediatric inflammatory bowel disease
- Author
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Aloi, M, Lionetti, P, Barabino, A, Guariso, Graziella, Costa, S, Fontana, M, Romano, C, Lombardi, G, Staiano, A, Alvisi, P, Diaferia, P, Baldi, M, Romagnoli, V, Gasparetto, M, DI PAOLA, M, Muraca, M, Pellegrino, S, Cucchiara, S, Martelossi, S, and on behalf of SIGENP IBD Group
- Published
- 2012
3. Compliant multistable structural elements
- Author
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Santer, M., Pellegrino, S., Santer, M., and Pellegrino, S.
- Abstract
Compliant multistable structures are presented which exhibit a large geometric change when actuated between their stable states. It is demonstrated how asymmetric-bistability is achieved through the combination of linear and nonlinear springs. Finite element analytical techniques are provided which enable the design of such structures, and which illustrate how the presence of imperfections can substantially alter their structural performance. A multistable structure is developed which consists of four connected bistable tetrahedral units. The validity of the analytical techniques is confirmed through observation of several physical models.
- Published
- 2008
4. Weld quality prediction in linear friction welding of AA6082-T6 through an integrated numerical tool
- Author
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Davide Campanella, Livan Fratini, Gianluca Buffa, Sergio Pellegrino, Buffa, G., Campanella, D., Pellegrino, S., and Fratini, L.
- Subjects
0209 industrial biotechnology ,Engineering ,Aluminum alloy ,Field (physics) ,Interface (computing) ,Neural Network ,02 engineering and technology ,Welding ,Industrial and Manufacturing Engineering ,law.invention ,020901 industrial engineering & automation ,Quality (physics) ,law ,Friction welding ,Settore ING-IND/16 - Tecnologie E Sistemi Di Lavorazione ,FEM ,Artificial neural network ,business.industry ,Oscillation ,Metals and Alloys ,Structural engineering ,021001 nanoscience & nanotechnology ,Finite element method ,Computer Science Applications ,Modeling and Simulation ,Ceramics and Composites ,Linear Friction Welding ,0210 nano-technology ,business - Abstract
A numerical and an experimental campaign were carried out with varying oscillation frequency and interface pressure. The local values of the main field variables at the contact interface between the specimens were predicted by a Lagrangian, implicit, thermo-mechanical FEM model and used as input of a dedicated Neural Network (NN). The NN, integrated in the FEM environment, was designed in order to calculate both a Boolean output, indicating the occurrence of welding, and a continuous output, indicating the quality of the obtained solid state weld. The analysis of the obtained results allowed three different levels of bonding quality, i.e., no weld, sound weld and excess of heat, to be correctly identified and predicted.
- Published
- 2016
5. Lactose malabsorption and intolerance: What is the correct management in older adults?
- Author
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Gallo A, Pellegrino S, Lipari A, Pero E, Ibba F, Cacciatore S, Marzetti E, Landi F, and Montalto M
- Subjects
- Humans, Aged, Animals, Milk, Diet, Lactase genetics, Lactose, Lactose Intolerance diagnosis, Gastrointestinal Diseases complications, Malabsorption Syndromes complications
- Abstract
Lactose malabsorption is a very common condition due to intestinal lactase deficiency. Post weaning, a genetically programmed and irreversible reduction of lactase activity occurs in the majority of the world's population. Lactose malabsorption does not necessarily result in gastrointestinal symptoms, i.e. lactose intolerance, which occurs in approximately one third of those with lactase deficiency. In the absence of well-established guidelines, the common therapeutic approach tends to exclude milk and dairy products from the diet. However, this strategy may have serious nutritional disadvantages. Mainly in particular categories, such as the older adults, the approach to lactose malabsorption may deserve careful considerations. Milk and dairy products are an important supply of a wide range of nutrients that contribute to meet the nutritional needs in different life stages. Dietary composition can significantly impact the mechanisms leading to age-related loss of bone mineral density, skeletal muscle mass or function and overall risk of sarcopenia. Moreover, in the latest years, different lines of evidence have highlighted an association between dairy intake and prevention of chronic diseases as well as all-cause mortality. The aim of this opinion paper is to provide an overview of lactose malabsorption and intolerance in the older adults and their implications in clinical practice., (Copyright © 2023 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.)
- Published
- 2023
- Full Text
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6. Hepatic angiomyolipoma: an international multicenter analysis on diagnosis, management and outcome.
- Author
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Klompenhouwer AJ, Dwarkasing RS, Doukas M, Pellegrino S, Vilgrain V, Paradis V, Soubrane O, Beane JD, Geller DA, Nalesnik MA, Tripke V, Lang H, Schmelzle M, Pratschke J, Schöning W, Beal E, Sun S, Pawlik TM, de Man RA, and Ijzermans JNM
- Subjects
- Adult, Angiomyolipoma mortality, Databases, Factual, Female, Hepatectomy, Humans, Liver Neoplasms mortality, Magnetic Resonance Imaging, Male, Middle Aged, Retrospective Studies, Tomography, X-Ray Computed, Treatment Outcome, Angiomyolipoma diagnosis, Angiomyolipoma therapy, Liver Neoplasms diagnosis, Liver Neoplasms therapy
- Abstract
Background: Hepatic angiomyolipoma (HAML) may easily be misdiagnosed as a malignancy. The study aim was to assess diagnostic dilemmas, clinical management and outcome of this rare tumor., Methods: This retrospective international multicenter study included all patients with pathologically proven HAML diagnosed between 1997 and 2017. Data on patient characteristics, diagnostic work-up, management and follow-up were analyzed., Results: Thirty-eight patients were included, 32 female. Median age was 56yrs (i.q.r. 43-64) and median HAML-diameter was 57.5 mm (i.q.r. 38.5-95.3). Thirty patients had undergone CT and 27/38 MRI of the liver, diagnostic biopsy was performed in 19/38. Initial diagnosis was incorrect in 15/38 patients, of which 13 were thought to have malignancy. In 84% biopsy resulted in a correct preoperative diagnosis. Twenty-nine patients were managed with surgical resection, 4/38 with surveillance and 3/38 with liver transplantation. Recurrence after resection occurred in two cases. No HAML related deaths or progression to malignancy were documented., Conclusion: HAML diagnosis proved problematic even in hepatobiliary expertise centers. Biopsy is indicated and may provide valuable additional information when HAML diagnosis is considered on cross-sectional imaging, especially when surgical resection imposes a risk of complications. Conservative management with regular imaging follow-up might be justified when biopsy confirms (classic type) HAML., (Copyright © 2019 International Hepato-Pancreato-Biliary Association Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
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7. Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
- Author
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Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, and Hashem Y
- Subjects
- Cryoelectron Microscopy, Histidine chemistry, Histidine metabolism, Hydrolysis, Models, Molecular, Peptide Elongation Factor 2 metabolism, Protein Conformation, RNA, Messenger metabolism, RNA, Transfer metabolism, Ribosomes metabolism, Guanosine Triphosphate metabolism, Histidine analogs & derivatives, Peptide Elongation Factor 2 chemistry, Protein Biosynthesis, RNA, Messenger chemistry, RNA, Transfer chemistry, Ribosomes chemistry, Saccharomyces cerevisiae metabolism
- Abstract
One of the most critical steps of protein biosynthesis is the coupled movement of mRNA, which encodes genetic information, with tRNAs on the ribosome. In eukaryotes, this process is catalyzed by a conserved G-protein, the elongation factor 2 (eEF2), which carries a unique post-translational modification, called diphthamide, found in all eukaryotic species. Here we present near-atomic resolution cryo-electron microscopy structures of yeast 80S ribosome complexes containing mRNA, tRNA and eEF2 trapped in different GTP-hydrolysis states which provide further structural insights into the role of diphthamide in the mechanism of translation fidelity in eukaryotes., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
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8. Stunting is an independent predictor of mortality in patients with cystic fibrosis.
- Author
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Vieni G, Faraci S, Collura M, Lombardo M, Traverso G, Cristadoro S, Termini L, Lucanto MC, Furnari ML, Trimarchi G, Triglia MR, Costa S, Pellegrino S, and Magazzù G
- Subjects
- Adolescent, Adult, Area Under Curve, Body Mass Index, Burkholderia cepacia, Case-Control Studies, Child, Child, Preschool, Cystic Fibrosis physiopathology, Female, Forced Expiratory Volume, Humans, Logistic Models, Male, Multivariate Analysis, Nutritional Status, Pseudomonas aeruginosa, Retrospective Studies, Risk Factors, Survival Rate, Body Height, Cystic Fibrosis mortality
- Abstract
Background & Aims: Some studies have shown a direct relationship between nutritional status and survival in Cystic Fibrosis (CF) patients. Body wasting, defined as a percentage of the ideal body weight for age, has been shown to be an independent predictor of mortality in CF. With respect to height only two studies were performed and these studies suggested that stunting is an important determinant of survival but both did not adjust statistical analysis for confounding variables. We aimed at determining the association between stunting and risk of mortality in CF patients., Methods: 393 CF patients older than 6 years of age, 95 deceased, as cases, and 298 live, as controls, were enrolled in a nested case-control study. Stunting was defined by a height percentile < 5th. We performed a multivariate statistical analysis including height percentile and the following possible confounding variables: age, gender, Body Mass Index (BMI), Forced Expiratory Volume in 1 s (FEV1), genotype, pancreatic status, CF-related diabetes, colonization with Pseudomonas aeruginosa and/or Burkholderia cepacia., Results: In the adjusted analyses stunting (OR 2.22 [IC 95%1.10-4.46]), wasting (OR 5.27 [IC 95% 2.66-10.41]), and FEV1 < 40% of predicted (OR 10.60 [IC 95% 5.43-20.67]) resulted the covariates that significantly predict the risk of mortality., Conclusions: Our study shows, for the first time, that stunting is a significant and independent risk factor for mortality in CF patients, and warrants an intervention of nutritional rehabilitation. Considering that nutritional interventions in stunted patients should be prolonged, are invasive and expensive, and might affect self-esteem and body image, their efficacy should be fully assessed by Randomised Controlled Trials., (Copyright © 2012 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.)
- Published
- 2013
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9. Three concentrations of levobupivacaine for ilioinguinal/iliohypogastric nerve block in ambulatory pediatric surgery.
- Author
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Disma N, Tuo P, Pellegrino S, and Astuto M
- Subjects
- Ambulatory Surgical Procedures methods, Anesthetics, Inhalation therapeutic use, Anesthetics, Local administration & dosage, Bupivacaine administration & dosage, Bupivacaine analogs & derivatives, Bupivacaine therapeutic use, Child, Child, Preschool, Dose-Response Relationship, Drug, Double-Blind Method, Female, Hernia, Inguinal surgery, Hospitals, University, Humans, Infant, Levobupivacaine, Male, Methyl Ethers therapeutic use, Pain Measurement, Prospective Studies, Sevoflurane, Anesthetics, Local therapeutic use, Nerve Block methods, Pain, Postoperative drug therapy
- Abstract
Study Objective: To compare the postoperative analgesia of three different concentrations of levobupivacaine for ilioinguinal/iliohypogastric (II/IH) block in children undergoing inguinal hernia repair., Design: Double-blind, prospective, randomized, controlled trial., Setting: Operating room and postoperative recovery area of a university hospital., Patients: 73 ASA physical status I and II children, aged one to 6 years, scheduled for outpatient inguinal hernia repair., Interventions: Patients were randomized to receive one of three levobupivacaine concentrations: 0.125% (L0.125), 0.25% (L0.25), or 0.375% (L0.375). All patients received standard anesthesia with sevoflurane and II/IH nerve block., Measurements: Heart rate (HR), non invasive blood pressure (NIBP), respiratory rate, end-tidal carbon dioxide concentration (ETCO(2)), and oxygen saturation via pulse oximetry (SpO(2)) were monitored during surgery. Postoperative pain scores with CHEOPS (Children's Hospital of Eastern Ontario Pain Scale) and need for rescue analgesia postoperatively were measured and recorded., Main Results: 60 patients entered the postoperative observational period. The number of patients who received rescue analgesia was comparable in the three groups. In Group L0.125, mean CHEOPS score was significantly higher, and time to first administration of rescue analgesia was shorter, than in the other two groups (P < 0.05). Pain scores and time to first administration of rescue analgesia were comparable between Groups L0.25 and L0.375., Conclusions: II/IH nerve block using 0.4 mL kg(-1) of 0.25% levobupivacaine provided satisfactory postoperative pain relief after inguinal herniorraphy.
- Published
- 2009
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10. Weight loss in obese type 2 diabetic patients on an intensive therapeutic programme: Importance of an initial short hospitalization period.
- Author
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Saldalamacchia G, Massaro P, Pacioni D, Giordano C, Tia VN, Pellegrino S, Lilli S, Riccardi G, and Rivellese AA
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- Adult, Combined Modality Therapy, Diabetes Mellitus, Type 2 diet therapy, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 physiopathology, Female, Humans, Hypoglycemic Agents therapeutic use, Inpatients, Length of Stay, Male, Middle Aged, Nutritional Physiological Phenomena, Obesity diet therapy, Obesity drug therapy, Obesity physiopathology, Outpatients, Patient Compliance, Program Evaluation, Treatment Outcome, Caloric Restriction, Diabetes Mellitus, Type 2 therapy, Exercise Therapy, Health Knowledge, Attitudes, Practice, Hospitalization, Obesity therapy, Patient Education as Topic, Weight Loss
- Published
- 2008
- Full Text
- View/download PDF
11. Expansile mandibular lesion in a child.
- Author
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Pellegrino SV and Berardi TR
- Subjects
- Adrenal Gland Neoplasms therapy, Child, Diagnosis, Differential, Humans, Male, Mandibular Neoplasms pathology, Mandibular Neoplasms therapy, Neoplasm Staging, Neuroblastoma pathology, Neuroblastoma therapy, Adrenal Gland Neoplasms pathology, Mandibular Neoplasms secondary, Neuroblastoma secondary
- Published
- 2000
- Full Text
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12. Liquid chromatographic determination of ethyl alcohol in body fluids.
- Author
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Pellegrino S, Bruno FS, and Petrarulo M
- Subjects
- Chromatography, High Pressure Liquid methods, Humans, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet, Ethanol blood
- Abstract
A high-performance liquid chromatographic technique for ethyl alcohol determination in body fluids is proposed. Ethyl alcohol is quantitatively converted into acetaldehyde-phenylhydrazone by oxidation in the presence of alcohol dehydrogenase, nicotinamide-adenine dinucleotide and phenylhydrazine. The derivative is suitable for reversed-phase liquid chromatography and ultraviolet detection at 276 nm. The limits of linearity, detection and quantification as well as accuracy and reproducibility were investigated in water, serum and whole blood. Analytical responses were linear within the 0.008 to 5 g/l range, and the limit of quantification was 0.02 g/l both in aqueous standard and in biological matrix assays. Mean analytical recovery of ethyl alcohol in blood serum averaged 98.2+/-4.2%, imprecision (CV%) at 0.80 g/l was 2.2%, and the limit of quantification was 0.02 g/l. Serum concentrations of persons that avoided alcoholic beverages for a week were less than the limit of quantification. Ethyl alcohol concentrations in serum and whole blood compared well with those obtained by headspace gas chromatography. This simple and reliable procedure, which was also used for a urine assay, could be suitable for validation of the screening procedures used to monitor ethanol abuse.
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- 1999
- Full Text
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13. High-performance liquid chromatographic microassay for L-glutamate:glyoxylate aminotransferase activity in human liver. Application in primary hyperoxaluria type 1.
- Author
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Petrarulo M, Pellegrino S, Marangella M, Cosseddu D, and Linari F
- Subjects
- Humans, Infant, Male, Middle Aged, Phenylhydrazines, Sensitivity and Specificity, Spectrophotometry, Ultraviolet, Chromatography, High Pressure Liquid methods, Hyperoxaluria, Primary enzymology, Liver enzymology, Transaminases metabolism
- Abstract
A rapid and sensitive liquid chromatographic technique to determine L-glutamate:glyoxylate and aminotransferase (EC 2.6.1.4) activity in human liver is described. Homogenised tissue was incubated for 60 min in the presence of substrates and the 2-oxoglutarate generated was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography. The procedure allowed the detection of the enzyme activity expressed by 7.5 micrograms of liver protein, it was more sensitive and less time-consuming than the spectrophotometric procedure previously used. No significant differences were found between normal controls and patients with primary hyperoxaluria. In an 8-month-infant with primary hyperoxaluria type 1, the enzyme activity was reduced to 16% of the average control values.
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- 1993
- Full Text
- View/download PDF
14. High-performance liquid chromatographic microassay for methyl ethyl ketone in urine as the 2,4-dinitrophenyl-hydrazone derivative.
- Author
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Petrarulo M, Pellegrino S, and Testa E
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- Humans, Hydrazones metabolism, Microchemistry, Butanones urine, Chromatography, High Pressure Liquid methods, Hydrazones urine
- Abstract
We report a high-performance liquid chromatographic procedure for determining methyl ethyl ketone in urine. The method is based on pre-column derivatization with 2,4-dinitrophenylhydrazine and liquid-liquid extraction of the derivative. The analyte is chromatographically separated from other urine constituents in less than 12 min and is detected by UV absorption at 360 nm. Peak height and concentration are linearly related. The relative standard deviation assessed for within-day imprecision was 3.2% at the 2.21 mg/l level. The mean analytical recovery from urines spiked with 1.0 mg/l ketone was 96.0 +/- 6.1%. The simple sample handling, the small volume of urine required and the short amount of time taken for the whole procedure make it suitable for routine biomonitoring of exposure to methyl ethyl ketone in industrial workers. The concentration in urine from nine non-exposed controls was less than 0.1 mg/l. The concentrations measured in urine samples from 60 exposed workers ranged from 0.1 to 1.1 mg/l and from 0.3 to 3.6 mg/l at the before- and the end-shift collections, respectively.
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- 1992
- Full Text
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15. High-performance liquid chromatographic microassay for L-alanine:glyoxylate aminotransferase activity in human liver.
- Author
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Petrarulo M, Pellegrino S, Marangella M, Cosseddu D, and Linari F
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- Alanine metabolism, Alanine Transaminase metabolism, Colorimetry, Glyoxylates metabolism, Humans, Kinetics, Microchemistry, Phenylhydrazines, Pyruvates metabolism, Pyruvic Acid, Spectrophotometry, Alanine Transaminase analysis, Chromatography, High Pressure Liquid methods, Hyperoxaluria enzymology, Liver enzymology, Transaminases
- Abstract
We examine the suitability of a rapid and sensitive liquid chromatographic technique to determine L-alanine:glyoxylate aminotransferase (AGT) activity in human liver. Homogenised tissue was incubated for 30 min in the presence of substrates and the generated pyruvate was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography (HPLC). The procedure allowed the detection of the enzyme activity expressed by 10 micrograms of liver protein and was rapid enough resulting more sensitive and less time-consuming than the previous colorimetric one. We found that AGT activity in two hyperoxaluria type 1 patients was reduced as compared with controls. Also, cirrhotic patients had very low enzyme activities, even in the absence of detectable disorders of oxalate metabolism and this was ascribed to abnormal liver morphology. This may represent a misleading drawback if diagnosis of type 1 primary hyperoxaluria (PH1) uniquely relies on AGT assay.
- Published
- 1992
- Full Text
- View/download PDF
16. Rapid high-performance liquid chromatographic determination of urinary N-(1-methylethyl)-N'-phenyl-1,4-benzenediamine in workers exposed to aromatic amines.
- Author
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Pellegrino S, Petrarulo M, Testa E, and Nicolotti A
- Subjects
- Chromatography, High Pressure Liquid methods, Environmental Monitoring methods, Humans, Amines adverse effects, Occupational Diseases chemically induced, Phenylenediamines urine
- Abstract
A procedure has been developed for determining N-(1-methylethyl)-N'-phenyl-1,4-benzenediamine in urine by using high-performance liquid chromatography. The method uses chloroform extraction for partial clean-up of the urine sample. The separation is carried out on a reversed-phase column using 65 mmol/l aqueous ammonium acetate in acetonitrile (30:70, v/v) as the mobile phase. The column effluent is monitored at 290 nm with an ultraviolet detector. The analyte is separated from other normal urine constituents in less than 4 min. Peak height and concentration are linearly related. Coefficients of variation assessed for within-day reproducibility were 5.9 and 3.7% at concentrations of 22.3 and 92.1 micrograms/l, respectively. The mean analytical recovery from urine samples spiked with known amounts of amine was 89.7 +/- 6.8%. The request of only a small volume of urine and the simple pre-treatment procedure makes it suitable for the routine monitoring of the exposure of rubber vulcanization workers to aromatic amines.
- Published
- 1992
- Full Text
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17. Improved high-performance liquid chromatographic determination of urinary glycolic acid.
- Author
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Petrarulo M, Bianco O, Cosseddu D, Marangella M, Pellegrino S, and Linari F
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- Adult, Alcohol Oxidoreductases, Chromatography, High Pressure Liquid statistics & numerical data, Humans, Reference Values, Chromatography, High Pressure Liquid methods, Glycolates urine, Hyperoxaluria, Primary urine
- Published
- 1990
- Full Text
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18. Ion chromatographic determination of plasma oxalate in healthy subjects, in patients with chronic renal failure and in cases of hyperoxaluric syndromes.
- Author
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Petrarulo M, Bianco O, Marangella M, Pellegrino S, Linari F, and Mentasti E
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- Adult, Ascorbic Acid blood, Chromatography, Ion Exchange, Glyoxylates blood, Humans, Indicators and Reagents, Reference Values, Ultrafiltration, Hyperoxaluria blood, Kidney Failure, Chronic blood, Oxalates blood
- Abstract
An ion chromatographic procedure for the determination of plasma oxalate is proposed, in which the ultrafiltered sample is injected into an ion-chromatographic system. Sample processing appears effective in avoiding spontaneous oxalogenesis. Sensitivity (down to 1.0 mumol/l) allows determinations in normal and pathological samples; recoveries from plasma ultrafiltration are 94.6 +/- 11.7%. Protein binding was investigated and precautions to improve recoveries from plasma ultrafiltration are proposed. The technique is simple to perform from healthy controls averaged 6.75 +/- 2.62 mumols/l (mean +/- S.D. n = 18); samples from patients with primary hyperoxaluria and chronic renal failure undergoing regular dialysis were also analysed and some of the data obtained are reported and discussed.
- Published
- 1990
- Full Text
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19. Extension of dental abscess to the orbit.
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Pellegrino SV
- Subjects
- Adolescent, Cellulitis etiology, Dental Caries complications, Edema etiology, Humans, Male, Periapical Abscess prevention & control, Tooth Extraction, Focal Infection, Dental complications, Orbit, Periapical Abscess complications
- Abstract
A week after dental treatment, a dentoalveolar abscess developed in a 14-year-old boy. Surgical intervention was needed to evacuate pus and remove the cause of infection. It is suggested that initially establishing drainage after dental treatment would have prevented the sequela that ensued.
- Published
- 1980
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20. High-performance liquid chromatographic determination of glyoxylic acid in urine.
- Author
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Petrarulo M, Pellegrino S, Bianco O, Marangella M, Linari F, and Metasti E
- Subjects
- Chromatography, High Pressure Liquid, Glyoxylates pharmacokinetics, Humans, Hydrogen-Ion Concentration, Indicators and Reagents, Phenylhydrazines urine, Spectrophotometry, Ultraviolet, Glyoxylates urine
- Abstract
A high-performance liquid chromatographic (HPLC) method for the determination of urinary glyoxylic acid is proposed. The system is based on the precolumn derivatization of alpha-keto acids by means of phenylhydrazine, separation of the phenylhydrazone formed by HPLC and spectrophotometric detection at 324 nm. The method is precise and allows the determination of 0.5 mumol/l glyoxylate. The poor stability of glyoxylate under all conventional preservation conditions requires the analysis to be carried out as soon as possible after urine collection. Results of determinations on urine samples from healthy controls and from patients with idiopathic calcium stone disease and type I primary hyperoxaluria are reported.
- Published
- 1988
- Full Text
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21. Derivatization and high-performance liquid chromatographic determination of urinary glycolic acid.
- Author
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Petrarulo M, Pellegrino S, Bianco O, Marangella M, Linari F, and Mentasti E
- Subjects
- Chromatography, High Pressure Liquid, Glyoxylates metabolism, Humans, Hydrazones urine, Hyperoxaluria urine, Glycolates urine
- Abstract
A high-performance liquid chromatographic method for the determination of urinary glycolic acid is proposed, based on pre-column derivatization with phenylhydrazine coupled with the enzymatic oxidation of glycolate to glyoxylate. The phenylhydrazone formed is separated by liquid chromatography and detected at 324 nm. The minimum detectable concentration of glycolate was 10.0 mumol/l. The recovery of glycolate added to urine averaged 96.1%. The day-to-day coefficients of variation calculated by analysis of two urine samples with normal and high glycolate contents were 4.6 and 7.5%, respectively. Results of analyses of urine samples from healthy persons, idiopathic calcium stone formers and Type I primary hyperoxaluria patients are reported.
- Published
- 1989
- Full Text
- View/download PDF
22. New category of drug allergy.
- Author
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Patriarca G, Romano A, Schiavino D, Nucera E, di Rienzo V, Pellegrino S, Fais G, and Romagna-Manoja E
- Subjects
- Humans, Radioallergosorbent Test, Skin Tests, Time Factors, Drug Hypersensitivity classification
- Published
- 1988
- Full Text
- View/download PDF
23. Pancuronium allergy: a case report.
- Author
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Patriarca G, Nucera E, Schiavino D, Romano A, Di Rienzo V, Pellegrino S, and Fais G
- Subjects
- Adult, Humans, Immunologic Tests, Male, Resuscitation, Skin Tests, Anaphylaxis chemically induced, Drug Hypersensitivity etiology, Pancuronium adverse effects
- Abstract
A case of anaphylactic shock to pancuronium bromide is described in which a type I immunological reaction occurred. A 33-yr-old male anaesthetist, with no family or personal history of allergy, collapsed soon after induction of anaesthesia. The drugs used included fentanyl, droperidol, thiopentone and pancuronium. Diffuse erythema occurred, with probable cardiac arrest. The patient was treated with cardiac massage, sympathomimetics and cortisone, and made a complete recovery. Examination (skin tests, passive transfer according to Prausnitz and Küstner, in vitro lymphocyte transformation test, total IgE assay) revealed that the allergic reaction was to pancuronium bromide.
- Published
- 1989
- Full Text
- View/download PDF
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