1. Active immunization against GnRH in pre-pubertal domestic mammals: testicular morphometry, histopathology and endocrine responses in rabbits, guinea pigs and ram lambs
- Author
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E. G. Sanchez-Cepeda, Manuel Garcia-Herreros, Miguel A. Gutierrez-Reinoso, and Pedro M. Aponte
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,endocrine system ,rabbits ,Biology ,Active immunization ,SF1-1100 ,Gonadotropin-Releasing Hormone ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Testis ,medicine ,Animals ,Endocrine system ,Body Weights and Measures ,Testosterone ,Vaccines, Contraceptive ,Hydrocortisone ,Sheep ,030219 obstetrics & reproductive medicine ,Vaccination ,Toxoid ,vaccines ,Sperm ,Animal culture ,030104 developmental biology ,Animals, Domestic ,GnRH ,Immunology ,Immunization ,Animal Science and Zoology ,Histopathology ,guinea pigs ,ram lambs ,Hormone ,medicine.drug - Abstract
Effective tools for male contraception are important in the control of reproduction in animal populations. The aim of the present study was to evaluate the effects of active immunization against gonadotropin-releasing hormone (GnRH) on male reproductive function assessing testicular morphological changes and serum-gonadotropin levels in pre-pubertal rabbits, guinea pigs and ram lambs. An anti-GnRH vaccine was developed by linking a GnRH-homologous molecule to a tetanus clostridial toxoid (Al(OH)3 coadjuvant). After vaccination protocols testicular morphometry, histopathological alterations and endocrine responses (FSH, LH, testosterone and cortisol serum levels) were evaluated. Testicular volume was significantly reduced in vaccinated animals with respect to the control group in rabbits, guinea pigs and ram lambs (P
- Published
- 2018