1. Functional inhibition of UQCRB suppresses angiogenesis in zebrafish.
- Author
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Cho YS, Jung HJ, Seok SH, Payumo AY, Chen JK, and Kwon HJ
- Subjects
- Amino Acid Sequence, Animals, Bridged Bicyclo Compounds pharmacology, Carrier Proteins genetics, Dose-Response Relationship, Drug, Embryo, Nonmammalian drug effects, Embryo, Nonmammalian physiology, Embryonic Development, Gene Knockdown Techniques, Mitochondria genetics, Mitochondria metabolism, Molecular Sequence Data, Morpholinos pharmacology, Prognosis, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Zebrafish genetics, Carrier Proteins metabolism, Gene Expression Regulation, Developmental, Neovascularization, Physiologic genetics, Zebrafish physiology
- Abstract
As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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