1. Characterization of a novel temperate phage originating from a cereulide-producing Bacillus cereus strain.
- Author
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Smeesters PR, Drèze PA, Bousbata S, Parikka KJ, Timmery S, Hu X, Perez-Morga D, Deghorain M, Toussaint A, Mahillon J, and Van Melderen L
- Subjects
- Bacillus Phages classification, Bacillus Phages physiology, Bacillus cereus genetics, Genome, Viral, Open Reading Frames, Prophages classification, Prophages genetics, Prophages isolation & purification, Prophages physiology, Siphoviridae classification, Siphoviridae physiology, Bacillus Phages genetics, Bacillus Phages isolation & purification, Bacillus cereus metabolism, Bacillus cereus virology, Depsipeptides metabolism, Siphoviridae genetics, Siphoviridae isolation & purification
- Abstract
A novel temperate bacteriophage was isolated from a Bacillus cereus cereulide-producing strain and named vB_BceS-IEBH. vB_BceS-IEBH belongs to the Siphoviridae family. The complete genome sequence (53 kb) was determined and annotated. Eighty-seven ORFs were detected and for 28, a putative function was assigned using the ACLAME database. vB_BceS-IEBH replicates as a plasmid in the prophage state. Accordingly, a 9-kb plasmid-like region composed of 13 ORFs was identified. A fragment of around 2000 bp comprising an ORF encoding a putative plasmid replication protein was shown to be self-replicating in Bacillus thuringiensis. Mass spectrometry analysis of the purified vB_BceS-IEBH particle identified 8 structural proteins and enabled assignment of a supplementary ORF as being part of the morphogenesis module. Genome analysis further illustrates the diversity of mobile genetic elements and their plasticity within the B. cereus group., (Copyright © 2011 Institut Pasteur. Published by Elsevier SAS. All rights reserved.)
- Published
- 2011
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