9 results on '"Olaya-Abril, Alfonso"'
Search Results
2. Contributors
- Author
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Adeolu, Adesiji R., primary, Agelaki, Katerina, additional, Alabi, Taofic, additional, Álvaro-Fuentes, Jorge, additional, Andriamananjara, Andry, additional, Andrisoa, Riana H., additional, Antón, Rodrigo, additional, Apesteguía, Marcos, additional, Arrúe, José L., additional, Badole, Shrikant, additional, Barman, Dhananjay, additional, Baumgarten, Andreas, additional, Bhattacharyya, Pratap, additional, Bielsa, Ana, additional, Birkás, Márta, additional, Blanco, Juan A., additional, Bodson, Bernard, additional, Brahim, Nadhem, additional, Brostaux, Yves, additional, Büyükkılıç-Yanardag, Asuman, additional, Cameron, Alison, additional, Almendro Candel, María B., additional, Cantero-Martínez, Carlos, additional, Carmona-Garcés, Dora M., additional, Cluzeau, Daniel, additional, Colinet, Gilles, additional, Datta, Ashim, additional, de Lima Dias Delarica, Denise, additional, de Melo, Gabriel M.P., additional, de Melo, Valéria P., additional, de Melo, Wanderley J., additional, de Sousa-Neto, Eráclito R., additional, Dencső, Márton, additional, Dersch, Georg, additional, Di Bene, Claudia, additional, Donha, Riviane M.A., additional, Escolano, Javier Jerez, additional, Farina, Roberta, additional, Faz, Ángel, additional, Faz-Cano, Ángel, additional, Francaviglia, Rosa, additional, Francis, Frédéric, additional, Franko, Uwe, additional, Frouz, Jan, additional, Garcia-Franco, Noelia, additional, Gelybó, Györgyi, additional, Gomes, Luciene, additional, Gómez-Garrido, Melisa, additional, Gründling, Ralf, additional, Haubruge, Eric, additional, Hewson, Jennifer, additional, Hobley, Eleanor, additional, Horel, Ágota, additional, Hübner, Rico, additional, Ibrahim, Hatem, additional, Jones, Julia P.G., additional, Jourdan, Christophe, additional, Kása, Ilona, additional, Kavvadias, Victor, additional, Koubouris, Georgios, additional, Krishna Chaitanya, Anantha, additional, Lemtiri, Aboulkacem, additional, Lozano-García, Beatriz, additional, Lucas, I. Gomez, additional, Majumder, Shyam Prasad, additional, Malliaraki, Stella, additional, Mandal, Biswapati, additional, Marchetti, Alessandro, additional, Martínez-Martínez, Silvia, additional, Martínez-Sánchez, Juan José, additional, Mohammad, Thamer A., additional, Moreno, José Luis, additional, Muñoz, María Ángeles, additional, Nascimento, Nathália, additional, Nik Daud, Nik N., additional, Obregón-Romero, Rafael, additional, Olaya-Abril, Alfonso, additional, Olivier, Claire, additional, Ometto, Jean P., additional, Ondoño, Sara, additional, Pacheco, Felipe, additional, Padhan, Dhaneshwar, additional, Papadopoulou, Maria, additional, Parras-Alcántara, Luis, additional, Pedreño, J. Navarro, additional, Piccini, Chiara, additional, Pierreux, Jérome, additional, Plante, Alain, additional, Plaza-Bonilla, Daniel, additional, Psarras, Georgios, additional, Raghubanshi, Akhilesh Singh, additional, Rajoelison, Gabrielle, additional, Ramananantoandro, Tahiana, additional, Ramboatiana, Nantenaina, additional, Ramifehiarivo, Nandrianina, additional, Ranaivoson, Ntsoa, additional, Rasolohery, Andriambolantsoa, additional, Razafimanantsoa, Marie P., additional, Razafimbelo, Tantely, additional, Razafinarivo, Ravo N.G., additional, Razafindrakoto, Mieja, additional, Razakamanarivo, Herintsitohaina, additional, Rory, Padfield, additional, Saint-André, Laurent, additional, Sandén, Taru, additional, Sayok, Alexander K., additional, Singh, Rishikesh, additional, Singh, Hema, additional, Spiegel, Heide, additional, Srivastava, Pratap, additional, Stephanie, Evers, additional, Terrero, Martire A., additional, Theocharopoulos, Sideris, additional, Tóth, Eszter, additional, Tripathi, Sachchidanand, additional, Troccoli, Antonio, additional, van Meerveld, Ilja, additional, Vavoulidou, Evangelia, additional, Vindušková, Olga, additional, Virto, Iñigo, additional, Wiesmeier, Martin, additional, Zornoza, Raúl, additional, and Zorpas, Antonis A., additional
- Published
- 2018
- Full Text
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3. Soil organic carbon distribution in Mediterranean areas under a climate change scenario via multiple linear regression analysis.
- Author
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Olaya-Abril A, Parras-Alcántara L, Lozano-García B, and Obregón-Romero R
- Abstract
Over time, the interest on soil studies has increased due to its role in carbon sequestration in terrestrial ecosystems, which could contribute to decreasing atmospheric CO
2 rates. In many studies, independent variables were related to soil organic carbon (SOC) alone, however, the contribution degree of each variable with the experimentally determined SOC content were not considered. In this study, samples from 612 soil profiles were obtained in a natural protected (Red Natura 2000) of Sierra Morena (Mediterranean area, South Spain), considering only the topsoil 0-25cm, for better comparison between results. 24 independent variables were used to define it relationship with SOC content. Subsequently, using a multiple linear regression analysis, the effects of these variables on the SOC correlation was considered. Finally, the best parameters determined with the regression analysis were used in a climatic change scenario. The model indicated that SOC in a future scenario of climate change depends on average temperature of coldest quarter (41.9%), average temperature of warmest quarter (34.5%), annual precipitation (22.2%) and annual average temperature (1.3%). When the current and future situations were compared, the SOC content in the study area was reduced a 35.4%, and a trend towards migration to higher latitude and altitude was observed., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
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4. Proteomics-driven design of a multiplex bead-based platform to assess natural IgG antibodies to pneumococcal protein antigens in children.
- Author
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Jiménez-Munguía I, van Wamel WJ, Olaya-Abril A, García-Cabrera E, Rodríguez-Ortega MJ, and Obando I
- Subjects
- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Male, Antibodies, Bacterial blood, Antigens, Bacterial, Immunoglobulin G blood, Pneumococcal Vaccines administration & dosage, Proteomics
- Abstract
Pneumococcal surface proteins are potential candidates for the development of protein-based vaccines and serological assays. The objective of the study was to develop a multiple bead-based immunoassay using Luminex xMAP® technology for the quantitation of natural antibodies against Streptococcus pneumoniae proteins and the characterization of the acute serum response following pneumococcal pneumonia in children. Sixty-four recombinantly produced pneumococcal proteins, which were selected based on their proteomic experimental identification by "shaving" live cells with trypsin followed by LC/MS/MS analysis, were coupled to fluorescent SeroMAP® beads and anti-pneumococcal specific IgG levels were determined in sera. Multiplex assay was validated through comparison of IgG levels to 14 randomly chosen pneumococcal antigens by using multiplex and singleplex assays. Acute serum IgG levels against RrgB were significantly lower in children ≤ 4 years old with pneumococcal pneumonia than those in controls. In addition, there was a small trend toward slightly lower antibody levels for PrsA, RrgC and RrgB in pneumonia patients of the all age group., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
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5. Characterization of protective extracellular membrane-derived vesicles produced by Streptococcus pneumoniae.
- Author
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Olaya-Abril A, Prados-Rosales R, McConnell MJ, Martín-Peña R, González-Reyes JA, Jiménez-Munguía I, Gómez-Gascón L, Fernández J, Luque-García JL, García-Lidón C, Estévez H, Pachón J, Obando I, Casadevall A, Pirofski LA, and Rodríguez-Ortega MJ
- Subjects
- Animals, Anti-Bacterial Agents chemistry, Cell Membrane metabolism, Child, Preschool, Chromatography, Chromatography, Liquid, Computational Biology, Databases, Protein, Drug Resistance, Bacterial, Fatty Acids chemistry, Female, Flow Cytometry, Humans, Male, Mice, Mice, Inbred BALB C, Pneumococcal Infections drug therapy, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Pneumococcal Infections blood, Streptococcus pneumoniae metabolism
- Abstract
Extracellular vesicles are produced by many pathogenic microorganisms and have varied functions that include secretion and release of microbial factors, which contribute to virulence. Very little is known about vesicle production by Gram-positive bacteria, as well as their biogenesis and release mechanisms. In this work, we demonstrate the active production of vesicles by Streptococcus pneumoniae from the plasma membrane, rather than being a product from cell lysis. We biochemically characterized them by proteomics and fatty acid analysis, showing that these vesicles and the plasma membrane resemble in essential aspects, but have some differences: vesicles are more enriched in lipoproteins and short-chain fatty acids. We also demonstrate that these vesicles act as carriers of surface proteins and virulence factors. They are also highly immunoreactive against human sera and induce immune responses that protect against infection. Overall, this work provides insights into the biology of this important Gram-positive human pathogen and the role of extracellular vesicles in clinical applications., Biological Significance: Pneumococcus is one of the leading causes of bacterial pneumonia worldwide in children and the elderly, being responsible for high morbidity and mortality rates in developing countries. The augment of pneumococcal disease in developed countries has raised major public health concern, since the difficulties to treat these infections due to increasing antibiotic resistance. Vaccination is still the best way to combat pneumococcal infections. One of the mechanisms that bacterial pathogens use to combat the defense responses of invaded hosts is the production and release of extracellular vesicles derived from the outer surface. Little is known about this phenomenon in Gram-positives. We show that pneumococcus produces membrane-derived vesicles particularly enriched in lipoproteins. We also show the utility of pneumococcal vesicles as a new type of vaccine, as they induce protection in immunized mice against infection with a virulent strain. This work will contribute to understand the role of these structures in important biological processes such as host-pathogen interactions and prevention of human disease., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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6. Surfomics: shaving live organisms for a fast proteomic identification of surface proteins.
- Author
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Olaya-Abril A, Jiménez-Munguía I, Gómez-Gascón L, and Rodríguez-Ortega MJ
- Subjects
- Animals, Humans, Bacterial Proteins metabolism, Gram-Negative Bacteria metabolism, Gram-Negative Bacterial Infections metabolism, Gram-Positive Bacteria metabolism, Gram-Positive Bacterial Infections metabolism, Proteomics methods
- Abstract
Surface proteins play a critical role in the interaction between cells and their environment, as they take part in processes like signaling, adhesion, transport, etc. In pathogenic microorganisms, they can also participate in virulence or cytotoxicity. As these proteins have the highest chances to be recognized by the immune system, they are often the targets for the discovery of new vaccines. In addition, they can serve for the development of serological-based tools to diagnose infectious diseases. First-generation proteomic strategies for the identification of surface proteins rely on the biochemical fractionation and/or enrichment of this group of molecules or organelles containing them. However, in the last years, a novel second-generation approach has been developed, consisting of the digestion of live, intact cells with proteases, so that surface-exposed moieties (i.e. the "surfome" of a cell) are "shaved" and analyzed by LC/MS/MS. Here we review such a strategy, firstly set up and developed in Gram-positive bacteria, and further applied to Gram-negative bacteria, unicellular fungi, and also pluricellular organisms. We also discuss the advantages and inconvenients of the approach, and the still unresolved question about the intriguing presence of proteins predicted as cytoplasmic in the surfomes. This article is part of a Special Issue entitled: Trends in Microbial Proteomics., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
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7. Exploring the pan-surfome of Streptococcus suis: looking for common protein antigens.
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Gómez-Gascón L, Luque I, Olaya-Abril A, Jiménez-Munguía I, Orbegozo-Medina RA, Peralbo E, Tarradas C, and Rodríguez-Ortega MJ
- Subjects
- Animals, Antibodies, Bacterial genetics, Antibodies, Bacterial immunology, Antigens, Bacterial genetics, Bacterial Proteins genetics, Bacterial Proteins immunology, Deoxyribonucleases genetics, Female, Membrane Proteins genetics, Membrane Proteins immunology, Membrane Proteins isolation & purification, Mice, Streptococcal Infections genetics, Streptococcal Infections immunology, Streptococcal Vaccines immunology, Streptococcus suis genetics, Swine, Tandem Mass Spectrometry, Deoxyribonucleases immunology, Streptococcal Infections veterinary, Streptococcus suis immunology
- Abstract
Streptococcus suis is a swine and human pathogen for which no commercial vaccine is still available. Conserved and broadly distributed surface proteins have become the chosen targets for the development of efficacious vaccines that could overcome the problems of non-heterologous protection of bacterins or capsule polysaccharide-based vaccines. In this work, we have analyzed by proteomics a collection of 39 strains obtained from infected pigs. The isolates belonged to 19 of the most prevalent serotypes during the last years. We have applied the "shaving" approach to define the "pan-surfome" or the set of both common and unique surface proteins identified in such strains. This set was constituted by 113 proteins. We have categorized them for their potential for further vaccination studies, based on their distribution among strains and their a priori accessibility to antibodies. According to these criteria, the cell-wall protein SsnA appears to be the best candidate from this list, as it was that with the widest distribution among the analyzed pathogen types, it showed to be highly immunogenic and highly accessible to antibodies, as demonstrated by flow cytometry., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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8. Another turn of the screw in shaving Gram-positive bacteria: Optimization of proteomics surface protein identification in Streptococcus pneumoniae.
- Author
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Olaya-Abril A, Gómez-Gascón L, Jiménez-Munguía I, Obando I, and Rodríguez-Ortega MJ
- Subjects
- Cell Membrane chemistry, Membrane Proteins analysis, Membrane Proteins chemistry, Peptide Mapping methods, Bacterial Proteins chemistry, Bacterial Proteins isolation & purification, Cell Fractionation methods, Mass Spectrometry methods, Membrane Proteins isolation & purification, Peptide Hydrolases chemistry, Streptococcus pneumoniae metabolism
- Abstract
Bacterial surface proteins are of outmost importance as they play critical roles in the interaction between cells and their environment. In addition, they can be targets of either vaccines or antibodies. Proteomic analysis through "shaving" live cells with proteases has become a successful approach for a fast and reliable identification of surface proteins. However, this protocol has not been able to reach the goal of excluding cytoplasmic contamination, as cell lysis is an inherent process during culture and experimental manipulation. In this work, we carried out the optimization of the "shaving" strategy for the Gram-positive human pathogen Streptococcus pneumoniae, a bacterium highly susceptible to autolysis, and set up the conditions for maximizing the identification of surface proteins containing sorting or exporting signals, and for minimizing cytoplasmic contamination. We also demonstrate that cell lysis is an inherent process during culture and experimental manipulation, and that a low level of lysis is enough to contaminate a "surfome" preparation with peptides derived from cytoplasmic proteins. When the optimized conditions were applied to several clinical isolates, we found the majority of the proteins described to induce protection against pneumococcal infection. In addition, we found other proteins whose protection capacity has not been yet tested. In addition, we show the utility of this approach for providing antigens that can be used in serological tests for the diagnosis of pneumococcal disease., (Copyright © 2012 Elsevier B.V. All rights reserved.)
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- 2012
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9. A surface protein of Streptococcus suis serotype 2 identified by proteomics protects mice against infection.
- Author
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Mandanici F, Gómez-Gascón L, Garibaldi M, Olaya-Abril A, Luque I, Tarradas C, Mancuso G, Papasergi S, Bárcena JA, Teti G, Beninati C, and Rodríguez-Ortega MJ
- Subjects
- Animals, Bacterial Proteins therapeutic use, Membrane Proteins therapeutic use, Mice, Proteomics, Streptococcal Infections prevention & control, Streptococcal Vaccines immunology, Streptococcus suis immunology, Swine, Bacterial Proteins immunology, Membrane Proteins immunology, Streptococcal Infections veterinary, Streptococcal Vaccines therapeutic use, Swine Diseases prevention & control
- Abstract
Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
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