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Parameters for wound age estimation are essential in forensic pathology but difficult to assess. With this retrospective study, wound age parameters in canine skin wounds were assessed with archive material of known age. Haematoxylin and eosin (HE) staining as standard, Prussian blue, Van Gieson (VG) and multiple other special stains were used as well as various immunohistochemistry (IHC) tests. Collagen fibre formation examination included HE staining but also immunolabelling for elastin and collagen I. Collagen fibre formation was further assessed by polarization in HE and VG. HE staining, erythrophagocytosis and presence of haemoglobin decay products in the Prussian blue stained-slides proved to be useful tools in wound age estimation in the early phase of wound repair of less than 5 days. The earliest detection of newly formed collagen was possible in 5-day-old wounds with HE and VG staining. Collagen I reactivity by IHC was weak at this time point, moderate to strong in the lesions older than 10 days and up to 30 days and strong in the lesions older than 3 months. Very slight polarization of collagen fibres was observed at 6 days, becoming stronger in wound lesions of 10-14 days and up to 30 days and reaching the same intensity as normal tissue in a 3-month-old lesion. The morphology of newly formed collagen fibres, utilizing HE and VG staining and polarization as well as collagen I IHC, which proved to be the most important parameters, correlated with distinguishable time points in the available cases of known wound age., Competing Interests: Declaration of competing interests The authors declared no conflicts in relation to the research, authorship and publication of this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Purpose: The primary aim of the present study is to examine the reasons for adolescents' refusal to get vaccinated with the COVID-19 vaccine, and examine correlates of vaccination among adolescents aged 12-18 years in Israel., Methods: A total of 150 youth aged 12-18 years participated in the study. Following parental consent (30% response rate) from an online internet Israeli participants' pool, 150 youth completed the survey (50.5% response rate). Data were collected from May to June 2021., Results: Over half (64.0%) of youth in this study had received the COVID-19 vaccine (25.5% received one dose and 38.9% two doses). Of the youth who were not vaccinated the most common reasons cited for refusing the vaccine was not knowing enough about the harms that a vaccine has in the long run, not trusting the drug companies that the vaccine will be safe, believing the virus is not dangerous, and doubting the safety of the vaccine in the short term. Bivariate odds ratios indicate that age (older) and having both parents vaccinated was related to increase the odds of the youth getting vaccinated. Higher distress over the effects of the vaccine was significantly related to lower odds of receiving the vaccine. Social media use was also related to a higher likelihood of being vaccinated at the bivariate level., Discussion: Study findings provide specific ways in which peer-designed and peer-led public health programs may encourage youth to receive the COVID-19 vaccine in a manner that recognizes concerns of Israeli youth., (Copyright © 2021 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.)