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Mycorrhizae and their hyphae play critical roles in soil organic carbon (SOC) accumulation. However, their individual contributions to SOC components and stability under climate warming conditions remain unclear. This study investigated the effects of warming on the SOC pools of Picea asperata (an ectomycorrhizal plant) and Fargesia nitida (an arbuscular mycorrhizal plant) mycorrhizae/hyphae on the eastern Tibetan Plateau. The results indicated that mycorrhizae made greater contributions to SOC accumulation than hyphae did by increasing labile organic carbon (LOC) components, such as particle organic carbon (POC), easily oxidizable organic carbon, and microbial biomass carbon, especially under warming conditions. Plant species also had different effects on SOC composition, resulting in higher mineral-associated organic carbon (MAOC) contents in F. nitida plots than in P. asperata plots; consequently, the former favored SOC stability more than the latter, with a lower POC/MAOC. Partial least-squares path modelling further indicated that mycorrhizae/hyphae indirectly affected LOC pools, mainly by changing soil pH and enzyme activities. Warming had no significant effect on SOC content but did change SOC composition by reducing LOC through affecting soil pH and iron oxides and ultimately increasing SOC stability in the presence of mycorrhizae for both plants. Therefore, the mycorrhizae of both plants are major contributors to the variation of SOC components and stability under warming conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Temperature sensitivity (Q 10 ) of soil microbial respiration serves as a crucial indicator for assessing the response of soil organic carbon (SOC) to global warming. However, the biogeographic variation in Q 10 remains inconsistent. In this study, we examined Q 10 and its potential drivers in nine old-growth mixed broad-leaved Korean pine (Pinus koraiensis Sieb. et Zucc.) forests (the climax community of Asian temperate mixed forest) under a wide range of climatic conditions. We found that stand characteristics (arbuscular mycorrhizal tree basal area to ectomycorrhizal tree basal area ratio and root to shoot ratio) contributed to soil C sequestration by facilitating the accumulation of soil recalcitrant C components. Contrary to the C quality-temperature hypothesis, Q 10 was not correlated with C quality (soil C to nitrogen ratio and recalcitrant C to labile C ratio). Soil mineral protection parameters (Fe/Al oxides) had negative effect on Q 10 because they inhibited microbial activities by decreasing substrate accessibility. Additionally, soils with high microbial biomass C and microbial biomass C to soil organic C ratio had high Q 10 . Overall, understanding the complex relationships among Q 10 , mineral protection, and microbial attributes on a spatial scale is essential for accurately predicting soil C cycling in forest ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Single phytoremediation has limited capacity to restore soil contaminated with extreme Mo due to its low metal accumulation. Soil organisms can help compensate for this deficiency in Mo-contaminated soils. However, there is limited information available on the integrated roles of different types of soil organisms, particularly the collaboration between soil microorganisms and soil animals, in phytoremediation. The objective of this study is to investigate the effects of a combination of arbuscular mycorrhizal fungi (AMF) and earthworms on the remediation of Mo-contaminated soils by alfalfa (Medicago sativa L.). The results indicated that in the soil-alfalfa system, earthworms effectively drive soil Mo activation, while AMF significantly improve the contribution of the translocation factor to total Mo removal (TMR) in alfalfas (p < 0.05). Meanwhile, compared to individual treatments, the combination of AMF and earthworm enhanced the expression of alfalfa root specific Mo transporter - MOT1 family genes to increase alfalfa uptake Mo (p < 0.05). This alleviated the competition between P/S nutrients and Mo on non-specific Mo transporters-P/S transporters (p < 0.05). Additionally, the proportion of organelle-bound Mo in the root was reduced to decrease Mo toxicity, while the cell wall-bound Mo proportion in the shoot was increased to securely accumulate Mo. The contributions of inoculants to alfalfa TMR followed the order (maximum increases): AMF + E combination (274.68 %) > alone treatments (130 %). Overall, the "functional division and cooperation" between earthworm and AMF are of great importance to the creation of efficient multi-biological systems in phytoremediation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The combined pollution of antibiotics and heavy metals (HMs) has a serious impact on the water ecological environment. Previous researches mainly focused on the removal of antibiotics or HMs as single pollutants, with limited investigation into the treatment efficiencies and underlying mechanisms associated with their co-occurring pollution. In this study, 16 micro vertical flow constructed wetlands (MVFCWs) were constructed to treat composite wastewater consisting of sulfamethoxazole (SMX), copper (Cu) and cadmium (Cd), involving two different inoculation treatments (arbuscular mycorrhizal fungi (AMF) inoculated and uninoculated) and eight kinds of pollutant exposure (Control Check (CK), SMX, Cu, Cd, SMX + Cu, SMX + Cd, Cu + Cd, SMX + Cu + Cd). The findings of this study demonstrated that the inoculation of AMF in MVFCWs resulted in removal efficiencies of SMX, Cu, and Cd ranging from 18.70% to 80.52%, 75.18% to 96.61%, and 40.50% to 89.23%, respectively. Cu and CuCd promoted the degradation of SMX in the early stage and inhibited the degradation of SMX in the later stage. Cd did not demonstrate a comparable promotive impact on SMX degradation, and its addition hindered Cu removal. However, comparatively, the presence of Cu exerted a more pronounced inhibitory effect on Cd removal. Furthermore, the addition of Cu augmented the abundances of Proteobacteria, Bacteroidetes (at the phylum level) and Rhodobacter, Lacunisphaera and Flavobacterium (at the genus level), and Cu exposure showed a substantially stronger influence on the microbial community than that of Cd and SMX. AMF might confer protection to plants against HMs and antibiotics by enriching Nakamurella and Lacunisphaera. These findings proved that AMF-C. indica MVFCW was a promising system, and the inoculation of AMF effectively enhanced the simultaneous removal of compound pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Lignite-converted bioorganic fertilizer substantially improves soil physiochemical properties, but little is known about how lignite bioorganic fertilizer (LBF) affects soil microbial communities and how the changed microbial communities impact their stability, functions, and crop growth in saline-sodic soil. Therefore, a two-year field experiment was conducted in saline-sodic soil in the upper Yellow River basin, Northwest China. Three treatments, i.e., the control treatment without organic fertilizer (CK), the farmyard manure treatment (FYM) amended with 21 t ha -1 (same as local farmers) sheep manure, and the LBF treatment amended with the optimal rate of LBF (3.0 and 4.5 t ha -1 ), were designed in this study. The results showed that after two years of application of LBF and FYM, the percentage of aggregate destruction (PAD) was significantly reduced by 14.4 % and 9.4 %, respectively, while the saturated hydraulic conductivity (K s ) was obviously increased by 114.4 % and 99.7 %, respectively. The LBF treatment significantly increased the contributions of nestedness to total dissimilarity by 101.4 % and 156.2 % in bacterial and fungal communities, respectively. LBF contributed to the shift from stochasticity to variable selection in the assembly of the fungal community. The LBF treatment enriched the bacterial classes of Gammaproteobacteria, Gemmatimonadetes, and Methylomirabilia and fungal classes of Glomeromycetes and GS13, which were mainly driven by PAD and K s . Additionally, the LBF treatment significantly increased the robustness and positive cohesions and decreased the vulnerability of the bacterial co-occurrence networks in both 2019 and 2020 in comparison with the CK treatment, indicating that the LBF treatment increased stability of bacterial community. The relative abundance of chemoheterotrophy and arbuscular mycorrhizae in the LBF treatment were 89.6 % and 854.4 % higher than those in the CK treatment, respectively, showing that the LBF enhanced sunflower-microbe interactions. The FYM treatment improved the functions mainly regarding sulfur respiration and hydrocarbon degradation by 309.7 % and 212.8 % in comparison with the CK treatment, respectively. The core rhizomicrobiomes in the LBF treatment showed strong positive connections with the stabilities of both bacterial and fungal co-occurrence networks, as well as the relative abundance and potential functions of chemoheterotrophy and arbuscular mycorrhizae. These factors were also linked to the growth of sunflowers. This study reveals that the LBF improved sunflower growth due to enhance microbial community stability and sunflower-microbe interactions through altering core rhizomicrobiomes in saline-sodic farmland., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Pesticides constitute an integral part of today's agriculture. Their widespread use leads to ubiquitous contamination of the environment, including soils. Soils are a precious resource providing vital functions to society - thus, it is of utmost importance to thoroughly assess the risk posed by widespread pesticide contamination. The exposure of non-target organisms to pesticides in soils is challenging to quantify since only a fraction of the total pesticide concentration is bioavailable. Here we measured and compared the bioavailable and total concentrations of three fungicides - boscalid, azoxystrobin, and epoxiconazole - and evaluated which concentration best predicts effects on nine microbial markers. The experiments were performed in three different soils at five time points over two months employing nearly 900 microcosms with a model plant. The total and bioavailable concentrations of azoxystrobin and boscalid decreased steadily during the trial to levels of 25 % and 8 % of the original concentration, respectively, while the concentration of epoxiconazole in soil nearly remained unchanged. The bioavailable fraction generally showed a slightly faster and more pronounced decline. The microbial markers varied in their sensitivity to the three fungicides. Specific microbial markers, such as arbuscular mycorrhizal fungi, and bacterial and archaeal ammonia oxidizers, were most sensitive to each of the fungicide treatments, making them suitable indicators for pesticide effects. Even though the responses were predominantly negative, they were also transient, and the impact was no longer evident after two months. Finally, the bioavailable fraction did not better predict the relationships between exposure and effect than the total concentration. This study demonstrates that key microbial groups are temporarily susceptible to a single fungicide application, pointing to the risk that repeated use of pesticides may disrupt vital soil functions such as nutrient cycling in agroecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Arbuscular mycorrhizal fungi were recovered from soil samples from the naturally radioactive soil at the long-abandoned South Terras uranium mine in Cornwall, UK. Species of Rhizophagus, Claroideoglomus, Paraglomus, Septoglomus, and Ambispora were recovered, and pot cultures from all except Ambispora were established. Cultures were identified to species level using morphological observation and rRNA gene sequencing combined with phylogenetic analysis. These cultures were used in pot experiments designed with a compartmentalised system to assess the contribution of fungal hyphae to the accumulation of essential elements, such as copper and zinc, and non-essential elements, such as lead, arsenic, thorium, and uranium into root and shoot tissues of Plantago lanceolata. The results indicated that none of the treatments had any positive or negative impact on shoot and root biomass. However, Rhizophagus irregularis treatments showed higher accumulation of copper and zinc in shoots, while R. irregularis and Septoglomus constrictum enhanced arsenic accumulation in roots. Moreover, R. irregularis increased uranium concentration in roots and shoots of the P. lanceolata plant. This study provides useful insight into fungal-plant interactions that determine metal and radionuclide transfer from soil into the biosphere at contaminated sites such as mine workings., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Nutrient resorption is an important mechanism for nutrient conservation and can maintain ecosystem stoichiometry. Here, we examined the global-scale variation of nitrogen resorption efficiency (NRE) and phosphorus resorption efficiency (PRE) by analyzing observations from 218 published papers. We used Pagel's λ to test the phylogenetic limitation on NRE and PRE and applied the random forest model to assess biotic and abiotic drivers, which included climate, soil, species characteristics, and topographical factors, and predicted the global NRE and PRE distributions. We found that NRE and PRE had oppositing trends among climatic zones, plant functional groups, and foliar nitrogen (N) to phosphorus (P) ratios. Nutrient resorption was higher in ectomycorrhizal trees than in arbuscular mycorrhizal trees. Moreover, foliar NRE and PRE were not linked to phylogeny. On average, the random forest overall explained 38 % (21 %-55 %) variation in NRE and 36 % (16 %-55 %) variation in PRE. Both NRE and PRE varied greatly with climate and soil organic carbon (SOC). The spatial variation of NRE and PRE was coupled to N-limitation and P-limitation, respectively. Our evaluation of the factors that influenced NRE and PRE and their global distributions, and our novel approach for evaluating plant utilization of nutrients, advances our understanding of the relative stability of ecosystem randomness in forest ecosystems and the global forest nutrient cycle., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

The rhizosphere is one of the most dynamic interfaces on the Earth. Understanding the magnitudes of rhizosphere effects (RE, difference in bio-physicochemical properties between rhizosphere and bulk soils) on soil microbial communities and their moderators is important for studying on below-ground carbon (C) cycling. A comprehensive meta-analysis was conducted to quantify the REs on soil microbial biomass, community structure, respiration, and C-degrading enzymes. We found that REs on soil C and nutrients, total microbial biomass, the abundance of specific microbial groups, fungi to bacteria ratio, respiration, and C-degrading enzymes were positive, but the magnitudes were varied with biomes, plant functional types, and mycorrhizal types. REs on microbial biomass, respiration, and C-degrading enzymes increased with the increase of mean annual temperature and mean annual precipitation, but decreased with the increase of soil clay, C, nitrogen (N), and phosphorus (P) contents. The REs on microbial biomass and respiration also increased as the REs on soil C:N:P increased. Compared with bulk soil, per unit rhizosphere soil C supported more microbial biomass, per unit of which respired more C, leading to faster C decomposition in rhizosphere. Our findings indicate that the increase in microbial biomass, co-metabolism induced by labile and energy-rich organic C of root exudates, and overflow respiration induced by stoichiometric imbalance together contribute to the enhanced C decomposition in rhizosphere. The global pattern of REs on soil microbial communities is critical to revealing the plant-microbe-soil interactions in terrestrial ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier B.V.)

Excessively high concentrations of selenium (Se) in soil are toxic to crop plants, and inoculation with arbuscular mycorrhizal fungi (AMF) can reverse Se stress in maize (Zea mays L.). To investigate the underlying mechanisms, maize seedlings were treated with sodium selenate (5 mg Se[VI] kg -1 ) and/or AMF (Funneliformis mosseae and Claroideoglomus etunicatum). Dual RNA sequencing in mycorrhiza and 16 S ribosomal DNA sequencing in soil were performed. The results showed that Se(VI) application alone decreased plant dry weight, but increased plant Se concentration, total Se content (mainly selenocysteine), and root superoxide content. Inoculation with either F. mosseae or C. etunicatum increased plant dry weight, decreased Se accumulation and selenocysteine proportion, enhanced root peroxidase activity, and alleviated oxidative stress in Se(VI)-treated plants. Inoculation also downregulated the expression of genes encoding Se transporters, assimilation enzymes, and cysteine-rich receptor-like kinases in Se(VI)-stressed plants, similar to plant-pathogen interaction and glutathione metabolism related genes. Conversely, genes encoding selenium-binding proteins and those related to phenylpropanoid biosynthesis were upregulated in inoculated plants under Se(VI) stress. Compared with Se(VI)-free plants, Se tolerance index, symbiotic feedback percentage on plant dry weight, and root colonization rate were all increased in inoculated plants under Se(VI) stress, corresponding to upregulated expression of 'key genes' in symbiosis. AMF inoculation increased bacterial diversity, decreased the relative abundances of selenobacteria related to plant Se absorption (e.g., Proteobacteria and Firmicutes), and improved bacterial network complexity in Se(VI)-stressed soils. We suggest that stress-mediated enhancement of mycorrhizal symbiosis contributed to plant Se(VI) tolerance, whereas AMF-mediated reshaping of soil bacterial community structure prevented excessive Se accumulation in maize., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Arbuscular mycorrhizal fungi (AMF) are ubiquitous in farmland. But the knowledge on AMF impact on lead (Pb) migration in farmland is limited. A field experiment was conducted in the rainy season (May-October) for two years in a Pb-polluted farmland. Benomyl was used to specifically suppress the native AMF growth in the farmland. The effect of benomyl-induced AMF suppression on the Pb uptake in maize, and Pb loss via surface runoff and interflows (20 cm and 40 cm depth) from the farmland was investigated. The benomyl significantly inhibited the AMF growth, resulting in decreases in the colonization rate, spore number, and contents of total and easily extractable glomalin-related soil protein (GRSP); and promoted the Pb migration into maize shoots and mainly enriched in leaves. The particulate Pb accounted for 83.2%-90.6% of Pb loss via surface runoff, while the proportion of particulate Pb loss via interflow was decreased and the proportion of dissolved Pb loss increased with the increase of soil depth. The AMF suppression led to a decrease in dissolved Pb concentration and loss, but an increase in particulate Pb concentration and loss, and enhanced the total Pb loss via surface runoff and interflows. Moreover, significant or very significant negative correlations were observed between the AMF colonization rate in roots with the Pb uptake in leaves, and the content of easily extractable GRSP with the particulate Pb loss. These results indicated the native AMF contributed to immobilizing Pb in soil and inhibited its migration to crops and the surrounding environment., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Fruit-bodies of six Thelephora species (Fungi, Basidiomycota, Thelephoraceae) were analyzed for their trace element concentrations. In Thelephora penicillata, extremely high concentrations of Cd and As were found, followed by highly elevated concentrations of Cu and Zn. The highest accumulation ability was found for Cd with a mean concentration of 1.17 ± 0.37 g kg -1 (dry mass) in fruit-bodies collected from 20 unpolluted sites; the mean As concentration was 0.878 ± 0.242 g kg -1 . Furthermore, striking accumulation of Se (923 ± 28 mg kg -1 ) was found in one sample of T. vialis and elevated concentrations of S were detected in T. palmata (19.6 ± 5.9 g kg -1 ). The analyzed Thelephora species were sequenced and, based on the Maximum Likelihood phylogenetic analysis (ITS rDNA) of the genus, possible other Thelephora (hyper)accumulators were predicted on the basis of their phylogenetic relationship with the discovered (hyper)accumulators. The striking ability of T. penicillata to accumulate simultaneously Cd, As, Cu, and Zn has no parallel in the Fungal Kingdom and raises the question of a biological importance of metal(loid) hyperaccumulation in mushrooms., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Achieving sustainable agricultural development requires the efficient use of nutrient resources for crop production. Recovering nutrients from animal manures may play a key role in achieving this. Animal manures typically have low nutrient concentrations, and in ratios that are often not ideal for balanced crop nutrition. Here, combinations of organic and inorganic phosphorus (P) were formulated as granular products (organomineral fertilisers) with granule size suitable for transport and spreading. The fertilisers were produced by granulating powdered chicken litter with MAP and urea powders making the following formulations: 0:4, 1:3, 2:2, 3:1, 4:0. The kinetics of NH 4 + -N and P release from the fertilisers, and the effects on tomato growth and nutrition, as well as arbuscular mycorrhizal formation in roots following fertiliser application, were determined. Cumulative NH 4 + -N release ceased within 12 h, and was lower in the formulations with higher proportions of chicken litter. The cumulative P released reached approximately 80% of total P in all formulations, and the time to obtain maximum P dissolution was 19 days in the formulation that contained only chicken litter. The organomineral fertilisers increased tomato shoot growth by 15-28% compared to the chicken litter only, MAP only and MAP/urea formulations. Reasonable levels of mycorrhizal colonisation of tomato roots was achieved with the organomineral fertilisers. The results demonstrated that optimum plant growth does not depend solely on immediately available P, and that timing of nutrient supply to match plant demand is important. The combination of chicken litter with MAP sustained nutrient supply and improved plant growth. Taken together, organomineral fertiliser formulations are potential alternatives to inorganic P fertilisers that can improve crop growth and nutrition, while provide a sustainable use for animal production wastes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

More than 80% terrestrial plants establish mutualistic symbiosis with soil-borne arbuscular mycorrhizal fungi (AMF). These fungi not only significantly improve plant nutrient acquisition and stress resistance, but also mitigate heavy metal phytotoxicity, Furthermore, the extraradical mycorrhizal mycelia can form common mycorrhizal networks (CMNs) that link roots of multiple plants in a community. Here we show that the networks mediate migration of heavy metal cadmium (Cd) from maize (Zea mays L.) to soybean (Glycine max (Linn.) Merr.) plants. CMNs between maize and soybean plants were established after inoculation of maize plants with AMF Funneliformis mosseae. Application of CdCl 2 in maize plants led to 64.4% increase in the shoots and 48.2% increase in the roots in Cd content in CMNs-connected soybean plants compared to the control without Cd treatment in maize. Meanwhile, although the CMNs-connected soybean plants did not directly receive Cd supply, they upregulated transcriptional levels of Cd transport-related genes HATPase and RSTK 2.13- and 5.96-fold, respectively, induced activities of POD by 44.8% in the leaves, and increased MDA by 146.2% in the roots . Furthermore, Cd addition inhibited maize growth but mycorrhizal colonization improved plant performance in presence of Cd stress. This finding demonstrates that mycorrhizal networks mediate the transfer of Cd between plants of different species, suggesting a potential to use CMNs as a conduit to transfer toxic heavy metals from main food crops to heavy metal hyperaccumulators via intercropping., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Nutrient loss from terrestrial ecosystems via leaching and gaseous emissions is increasingly threatening global environmental and human health. Although arbuscular mycorrhizal fungi (AMF) have been shown to regulate soil N and P losses, a comprehensive quantitative overview of their influences on the losses of these soil nutrients across global scales is currently lacking. This study used a meta-analysis of 322 observations from 36 studies to assess the effect of AMF inoculum on 11 variables related to the loss of soil N and P. We found that the presence of AMF significantly reduced soil N and P losses, with the most pronounced reduction occurring in soil NO 3 - -N (-32%), followed by total P (-21%), available P (-16%) and N 2 O (-10%). However, the mitigation effects of AMF on soil N and P loss were dependent on the identity of AMF inoculum, plant type and soil biotic and abiotic factors. Generally, the mitigation effects of AMF increased with increasing AMF root colonization rate, microbial diversity of inoculants, soil organic carbon (SOC) content and experimental duration as well as with decreasing soil sand contents and soil N and P availability. Overall, this meta-analysis highlights the importance of AMF inoculation in mitigating N and P nutrient loss and environmental pollution for terrestrial ecosystem sustainability., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021. Published by Elsevier B.V.)

Copper (Cu) contamination threatens the stability of soil ecosystems. As important moderators of biochemical processes and soil remediation, the fungal community in contaminated soils has attracted much research interest. In this study, soil fungal diversity and community composition under long-term Cu contamination were investigated based on high-throughput sequencing. The co-occurrence networks were also constructed to display the co-occurrence patterns of the soil fungal community. The results showed that the richness and Chao1 index both significantly increased at 50 mg kg -1 Cu and then significantly decreased at 1600 and 3200 mg kg -1 Cu. Soil fungal diversity was significantly and positively correlated with plant dry weight. Specific tolerant taxa under different Cu contamination gradients were illustrated by linear discriminant analysis effect size (LEfSe). Soil Cu concentration and shoot dry weight were the strongest driving factors influencing fungal composition. The relative abundance of arbuscular mycorrhizal fungi increased first and then declined along with elevating Cu concentrations via FUNGuild analysis. The interactions among fungi were enhanced under light and moderate Cu contamination but weakened under heavy Cu contamination by random matrix theory (RMT)-based molecular ecological network analysis. Penicillium, identified as a keystone taxon in Cu-contaminated soils, had the function of removing heavy metals and detoxification, which might be vital to trigger the resistance of the fungal community to Cu contamination. The results may facilitate the identification of Cu pollution indicators and the development of in situ bioremediation technology for contaminated cultivated fields., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

A considerable amount of data has been published on the accumulation of radiocaesium ( 134 Cs and particularly, 137 Cs) in wild fungi since the first anthropogenically influenced releases into the environment due to nuclear weapon testing, usage and subsequently from major accidents at nuclear power plants in Chernobyl (1986) and Fukushima (2011). Wild fungi are particularly susceptible to accumulation of radiocaesium and contamination persists for decades after pollution events. Macromycetes (fruiting bodies, popularly called mushrooms) of the edible fungal species are an important part of the human and forest animal food-webs in many global locations. This review discusses published occurrences of 134 Cs and 137 Cs in twenty four species of Tricholoma mushrooms sourced from the Northern Hemisphere over the last five decades, but also includes some recent data from Italy and Poland. Tricholoma are an ectomycorrhizal species and the interval for contamination to permeate to lower soils layers which host their mycelial networks, results in a delayed manifestation of radioactivity. Available data from Poland, over similar periods, may suggest species selective differences in accumulation, with some fruiting bodies, e.g. T. portentosum, showing lower activity levels relative to others, e.g. T. equestre. Species like T. album, T. sulphurescens and T. terreum also show higher accumulation of radiocaesium, but reported observations are few. The uneven spatial distribution of the data combined with a limited number of observations make it difficult to decipher any temporal contamination patterns from the observations in Polish regions. When data from other European sites is included, a similar variability of 137 Cs activity is apparent but the more recent Ukrainian data appears to show relatively lower activities. 40 K activity in mushrooms which is associated with essential potassium, remains relatively constant. Further monitoring of 137 Cs activity in wild mushrooms would help to consolidate these observations., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

Cadmium (Cd) is a major environmental pollutant and one of the most toxic metals in the environment. Arbuscular mycorrhizal fungi (AMF) assisted phytoremediation can be used to remove Cd from polluted soils but the role of AMF, which mediate in Cd accumulation and tolerance, remains poorly understood. Here we inoculated Lolium perenne with two different AMF species (Glomus etunicatum and Glomus mosseae). Mycorrhizal L. perenne and non-mycorrhizal controls were exposed to Cd stress and we tested the effects of AMF mycorrhization on Cd uptake and subsequent tolerance, as well as the underlying mechanisms. Mycorrhizal infection increased root Cd 2+ uptake and we observed that net Cd 2+ influx was coupled with net Ca 2+ influx. The inactivation of Ca 2+ transporter channels decreased Cd 2+ uptake in non-inoculated roots to a greater extent than in inoculated roots, indicating that AMF activates additional ion transport channels. In consequence, inoculated plants exhibited higher Cd accumulation in both roots and shoots than non-inoculated controls. However, AMF-inoculated plants showed higher chlorophyll concentrations, photosynthesis, and growth under Cd, indicating lower Cd toxicity in AMF-inoculated plants, despite the increase in Cd uptake. We observed that AMF-inoculated favored the isolation of Cd within cell walls and vacuoles, and had higher concentrations of superoxide dismutase activity and glutathione concentration in roots than non-inoculated plants, consequently experiencing less stress upon Cd exposure. Our results highlight the potential and mechanism of AMF for enhancing phytoremediation of L. perenne in heavy metal contaminated environments., Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

In this study, we evaluated the impact of washing of Pb, Zn and Cd contaminated soil using EDTA-based technology (ReSoil®) on soil biological properties by measuring some of the most commonly used/sensitive biological indicators of soil perturbation. We estimated the temporal dynamics of the soil respiration, the activities of soil enzymes (dehydrogenase, β-glucosidase, urease, acid and alkaline phosphatase), and the effect of the remediation process on arbuscular mycorrhizal (AM) fungi in original (Orig), remediated (Rem) and remediated vitalized (Rem+V) soils during a more than one-year garden experiment. ReSoil® technology initially affected the activity level of soil microbial respiration and all enzyme activities except urease and reduced AM fungal potential in the soil. However, after one year of vegetable cultivation and standard gardening practices, soil microbial respiration, acid and alkaline phosphatase in the Rem and Rem+V reached similar activities as in the Orig. Only the activities of dehydrogenase and β-glucosidase remained lower in the remediated soil compared to the Orig. The frequency of arbuscular mycorrhiza in the root system, arbuscular density in the colonized root fragment, and the intensity of mycorrhizal colonization in the colonized root fragments in the remediated treatments increased with time; at the end of the experiment, no consistent differences in these parameters of mycorrhizal colonization were found among the treatments. Our results suggest a restored biological functioning of the remediated soil after one year of vegetable cultivation. In general, no differences were found between the Rem and Rem+V treatments, indicating that simple common garden practices are sufficient to restore soil functioning after remediation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

Understanding the combined effects of soil amendments and inoculation of mycorrhizal fungi on the response of different plant species during the phytostabilization process of trace elements contaminated soils is a challenge. This task is more difficult but more realistic when studied under field conditions. We assess the combined effects of two amendment doses and mycorrhizal inoculation on the response of saplings of two tree species planted in a contaminated field. The amendments were a mix of sugar beet lime and biosolid compost. The inoculation treatments were made with a commercial inoculum of arbuscular mycorrhizal fungi for wild olive and ectomycorrhizal fungi for stone pine. Results showed a weak or null effect of the mycorrhizal inoculation on plant growth, survival and trace element accumulation. There was a significant increase on P nutrition for stone pine, growing on non-amended conditions. Soil amendments were very effective reducing trace elements availability and their accumulation in both plant species, especially in roots. However, the effects on plant biomass were species-dependent and contrasted; low-dose amendments increased the biomass of wild olive by 33.3%, but reduced by 28% that of pine. The high doses of amendments (60 T ha -1 ) produced some negative effects on plant growth and nutrition, probably related to the increase of soil salinity. Both plant species, stone pine and wild olive, have been proved to be adequate for phytostabilization of contaminated soils under Mediterranean climate, due to their drought tolerance and the low transfer of trace elements from root to shoot, thus reducing toxicity for the food web. To implement microbial-assisted phytoremediation approaches, a better understanding of the diversity and ecology of plant-associated microorganisms is needed. The use of indigenous fungi, locally adapted and tolerant to contamination, would be more suitable for phytostabilization purposes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)

Modern breeding efforts have been accelerating crop improvement and yielding numerous cultivars with distinct genetic traits; however, interactions between different cultivars and their root-associated arbuscular mycorrhizal fungi (AMF) are not clear. Herein, we selected the 22 most common commercial maize (Zea mays) varieties in China and an inbred line (B73) to study the differential responses of these 23 cultivars to mycorrhizal inoculation when grown in an arable soil polluted by multiple metals (Pb, Zn, and Cd). We found that the different cultivars exhibited significant variations in plant metal accumulation, ranging from strong metal exclusion (ZYY9) to strong metal accumulation (B73). Mycorrhizal colonization substantially altered metal uptake and repartitioning, while bioaugmenting the inherent characteristics of metal accumulation; for example, the AMF enhanced leaf accumulation of the metal-accumulator B73, and markedly reduced the root uptake of the metal-excluder ZYY9. However, such AMF-induced alterations were also substantially dependent on plant organs (roots and shoots) and metal species. We found that the extent of the AMF-induced leaf alterations was substantially greater than that of the root alterations. Similarly, the number of instances where the AMF significantly altered the Zn and Cd accumulation was far higher than the number of instances where Pb accumulation was significantly altered by AMF. In addition, the presence of AMF appeared to trigger the maize antioxidant systems, which may have alleviated the toxicity of excessive Cd, increased the leaf chlorophyll content, augmented the net photosynthetic rate, and promoted the growth of 17.39% of the maize cultivars. Our results suggest that a future crop breeding challenge is to produce cultivars for safe production or phytoremediation, thereby optimizing the combinations of crop cultivars and their root-associated AMF in slightly to moderately metal-polluted arable soils., Competing Interests: Declaration of competing interest This work has no actual or potential conflict or interest including any financial personal or other relationships with other people or organizations., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Anthropogenic N emissions represent a potential threat for forest ecosystems, and environmental indicators that provide insight into the changing forest N cycle are needed. Tree ring N isotopic ratios (δ 15 N) appear as a contentious choice for this role as the exact mechanisms behind tree-ring δ 15 N changes seldom benefit from a scrutiny of the soil-to-tree N continuum. This study integrates the results from the analysis of soil chemistry, soil microbiome genomics, and δ 15 N values of soil N compounds, roots, ectomycorrhizal (EcM) fungi and recent tree rings of thirteen white spruce trees sampled in five stands, from two regions exposed to moderate anthropogenic N emissions (3.9 to 8.1 kg/ha/y) with distinctive δ 15 N signals. Our results reveal that airborne anthropogenic N with distinct δ 15 N signals may directly modify the NO 3 - δ 15 N values in surface soils, but not the ones of NH 4 + , the preferred N form of the studied trees. Hence, the tree-ring δ 15 N values reflect specific soil N conditions and assimilation modes by trees. Along with a wide tree-ring δ 15 N range, we report differences in: soil nutrient content and N transformation rates; δ 15 N values of NH 4 + , total dissolved N (TDN) and EcM mantle enveloping the root tips; and bacterial and fungal community structures. We combine EcM mantle and root δ 15 N values with fungal identification to infer that hydrophobic EcM fungi transfer N from the dissolved organic N (DON) pool to roots under acidic conditions, and hydrophilic EcM fungi transfer various N forms to roots, which also assimilate N directly under less acidic conditions. Despite the complexities of soil biogeochemical properties and processes identified in the studied sites, in the end, the tree-ring δ 15 N averages inversely correlate with soil pH and anthropogenic N inputs, confirming white spruce tree-ring δ 15 N values as a suitable indicator for environmental research on forest N cycling., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Crown Copyright © 2021. Published by Elsevier B.V. All rights reserved.)

Agricultural soils are an important source of nitrous oxide (N 2 O), a potent greenhouse gas involved in the destruction of the protective ozone layer that contributes to global warming. During N 2 O production, soil microorganisms play important driving and regulating roles. A few recent studies have revealed the potential effects of arbuscular mycorrhizal fungi (AMF), a widely distributed soil fungi, on controlling N 2 O emissions. However, how AMF regulate N 2 O production from soils remains poorly understood. To address the knowledge gap, we manipulated two independent soil environments, which were either allowed (AM) or prevented (NM) access by AMF hyphae in a microcosm experiment (n = 5). Soil physicochemical properties, N 2 O flux, the diversity of bacterial communities, and the abundance of key genes responsible for N 2 O production were assessed in both treatments over three months. Results showed that the presence of AMF significantly decreased N 2 O emissions from agricultural soils in the 1st month, and the abundance of key genes responsible for denitrification (nirK and nosZ) significantly decreased in AM treatments, indicating that the regulation of N 2 O emissions is transmitted by AMF-induced changes in the denitrification process. A structural equation model further revealed that AMF indirectly influenced N 2 O emissions by altering the abundance of N metabolism-related genes, rather than by altering soil chemical properties or the diversity of bacterial communities. Thus, we proposed a possible mechanism by which AMF can regulate denitrification activities and therefore N 2 O emissions from agricultural soils., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

As symbionts capable of reciprocal rewards, arbuscular mycorrhizal fungi (AMF) can alleviate heavy metal toxicity to host plants and are easily influenced by elevated CO 2 (ECO 2 ). Although the individual effects of ECO 2 and cadmium (Cd) on AMF have been widely reported, the response of AMF to ECO 2  + Cd receives little attention. We evaluated the combined effects of ECO 2 and Cd on AMF in the rhizosphere soil and roots of Robinia pseudoacacia L. seedlings. Under ECO 2  + Cd relative to Cd, AMF gene copies and richness in rhizosphere soils increased (p < 0.05) and the diversity reduced (p < 0.05) at 4.5 mg Cd kg -1 dry soil; whereas root AMF abundance at 4.5 mg Cd kg -1 dry soil and the diversity and richness reduced (p < 0.05). Elevated CO 2 caused obvious differences in the dominant genera abundance between rhizosphere soils and roots upon Cd exposure. Responses of C, water-soluble organic nitrogen (WSON), pH, and diethylene triamine penta-acetic acid (DTPA)-Cd in rhizosphere soils and root N to ECO 2 shaped dominant genera in Cd-polluted rhizosphere soils. Levels of DTPA-Cd, WSON, C and pH in rhizosphere soils and C/N ratio, N, and Cd in roots to ECO 2 affected (p < 0.05) dominant genera in roots under Cd exposure. AMF richness and diversity were lower in roots than in rhizosphere soils. Elevated CO 2 altered AMF communities in rhizosphere soils and roots of R. pseudoacacia seedlings exposed to Cd. AMF associated with R. pseudoacacia may be useful/interesting to be used for improving the phytoremediation of Cd under ECO 2 ., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The unregulated deposition of metal-based nanoparticles in terrestrial ecosystems particularly in agricultural systems has alarmingly threatened the sustainability of the environment and diversity of beneficial microbial populations such as soil bacteria and fungi. This occurs due to the poor treatment of biosolids during wastewater treatment and their application in agricultural fields to enhance the fertility of soils. Continuous deposition, low biodegradability, and longer persistence of metal nanoparticles in soils adversely impact the population of soil beneficial bacteria and fungi. The current literature suggests the toxic outcome of nanoparticle-fungi and nanoparticle-bacteria interactions based on various toxicity endpoints. Therefore, due to the extreme importance of beneficial soil bacteria and fungi for soil fertility and plant growth, this review summarizes the production, application, release of metal nanoparticles in the soil system and their impact on various soil microbes specifically plant growth-promoting rhizobacteria, cellular toxicity and impact of nanoparticles on bioactive molecule production by microbes, destructive nanoparticle impact on unicellular, mycorrhizal, and cellulose/lignin degrading fungi. This review also highlights the molecular alterations in fungi and bacteria-induced by nanoparticles and suggests a plausible toxicity mechanism. This review advances the understanding of the nano-toxicity aspect as a common outcome of nanoparticles and fungi/bacteria interactions., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Global change alters how terrestrial ecosystems function and makes them less stable over time. Global change can also suppress the development and effectiveness of arbuscular mycorrhizal fungi (AMF). This is concerning, as AMF have been shown to alleviate the negative influence of global changes on plant growth and maintain species coexistence. However, how AMF and global change interact and influence community temporal stability remains poorly understood. Here, we conducted a 4-year field experiment and used structural equation modeling (SEM) to explore the influence of elevated temperature, nitrogen (N) addition and AMF suppression on community temporal stability (quantified as the ratio of the mean community productivity to its standard deviation) in a temperate meadow in northern China. We found that elevated temperature and AMF suppression independently decreased the community temporal stability but that N addition had no impact. Community temporal stability was mainly driven by elevated temperature, N addition and AMF suppression that modulated the dominant species stability; to a lesser extent by the elevated temperature and AMF suppression that modulated AMF richness associated with community asynchrony; and finally by the N addition and AMF suppression that modulated mycorrhizal colonization. In addition, although N addition, AMF suppression and elevated temperature plus AMF suppression reduced plant species richness, there was no evidence that changes in community temporal stability were linked to changes in plant richness. SEM further showed that elevated temperature, N addition and AMF suppression regulated community temporal stability by influencing both the temporal mean and variation in community productivity. Our results suggest that global environmental changes may have appreciable consequences for the stability of temperate meadows while also highlighting the role of belowground AMF status in the responses of plant community temporal stability to global change., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The purpose of this work was to study the factors affecting the absorption of U by plants growing on the spoil tip of an abandoned mine in western Spain. The plant species were selected based on how palatable they were to livestock and were sampled for four consecutive years during which, we also recorded rainfall data. The factors related to the plants studied were the leaf size and the percentage and characteristics of the arbuscular mycorrhizae (AM) fungi present in their roots. Our results showed a correlation between the annual rainfall and the U concentration in the plants. The percentage of mycorrhization and AM vesicles is a predominant factor in the uptake of U by plants. Spergularia rubra (L.) J.Presl & C.Presl, which is resistant to mycorrhization, contained higher U concentrations relative to the plants that grew with AM mycorrhization. The absorption curves of the different plants studied indicated that these plants were tolerant to 238 U from 875 Bq kg -1 (70 mg kg -1 ), with a hormesis effect below that concentration. The annual U removal was 0.068%, suggesting that AM are responsible for limiting the incorporation of U into the food chain, favouring its retention in the soil and preventing its dispersion., Competing Interests: Declaration of competing interest The authors have no affiliation with any organization with a direct or indirect financial interest in the subject matter discussed in the manuscript., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Growing environmental concern regarding multi elements-contaminated soils reveals the necessity of paying more attention to environmentally friendly remediation techniques such as phytoremediation. A large number of factors influences phytoremediation of potentially toxic elements (PTEs) and investigation on a variety of these factors need appropriate statistical approaches such as "Taguchi optimization" which effectively decreases time and cost of experiments. In the present study, based on the Taguchi optimization method, the effects of several biological (plant type and mycorrhizal fungi (AMF)) and chemical (chelating agents, surfactants and organic acids) factors, on the phytoremediation of soils contaminated with zinc (Zn), lead (Pb), cadmium (Cd) and nickel (Ni) were investigated. The goal was to find out the most effective factors as well as the best level for each factor. The values of dry weights in roots and aerial parts of the studied plants were in orders of maize > sorghum > sunflower and sorghum > maize > sunflower, respectively. AMF was the main factor in increasing dry weight of shoots. Inoculation of AMF caused increases in root and shoot uptake of some PTEs. RESULTS: showed that phytoremediation of PTEs is element-dependent; as Zn showed the highest translocation factor (TF) and bioconcentration factor (BCF) values, while Ni showed the lowest ones and the intermediate values belonged to Pb and Cd. These results show the diverse distribution of elements in plant parts, as Zn and Ni were mostly accumulated in shoot and root, respectively. Although different factors caused impacts on phytoremediation criteria, the role of plant type in the phytoremediation of PTEs was at the first rank. Mean TF of PTEs in sunflower was 6.3 times that of maize. Sunflower showed high TF value for the four elements and translocated most of the PTEs from root to the aerial parts demonstrating phytoextraction as the main mechanism in this plant. Maize and sorghum, however, showed low TF and accumulated most of PTEs in their roots revealing phytostabilization as the main mechanism. In general, it can be concluded that plant type was the most influential factor in the phytoremediation of PTEs followed by EDTA and AMF. Taguchi optimization revealed the appropriateness and significance of different chemical and biological treatments on phytoremediation criteria of different elements., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Selenium (Se) deficiency is a public health concern that is mainly caused by inadequate intake of Se from staple crops. The purpose of this study is to investigate the effects of inoculation with different arbuscular mycorrhizal fungus (AMF) strains, including Funneliformis mosseae (Fm) and Glomus versiforme (Gv), and fertilization with selenite or selenate on the accumulation and speciation of Se in rice. The results showed that using both AMF inoculation and Se fertilization could promote organic Se accumulation in rice grain than using only Se fertilization. Moreover, grain of rice inoculated with Fm and grown in soil fertilized with selenate had the highest accumulation of Se, of which selenomethionine was the dominant Se species. The AMF inoculation also led to high content of available Se and high relative abundance of Firmicutes in soil. The high concentration of available Se in soil suggests that the AMF inoculation may modify the microbial community, which then causes the Se uptake of rice to increase, in turn causing the amount of organic Se accumulated in rice to increase. Based on these results, using AMF inoculation combined with Se fertilization can be a promising strategy for Se biofortification in rice., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Maize (Zea mays L.)/soybean (Glycine max L.) intercropping has been widely practiced in China, because of its effectiveness in improving crop yield and nitrogen utilization efficiency. However, the responses of indigenous arbuscular mycorrhizal fungal (AMF) diversity and communities in rhizosphere soil and roots to intercropping systems with different nitrogen application rates remain unclear. In this study, a field experiment was conducted with split-plot design, and AMF communities in crop rhizosphere soil and roots in monoculture and intercropping systems treated with different levels of nitrogen fertilization were investigated using Illumina MiSeq sequencing. Nitrogen fertilization significantly decreased the AMF alpha-diversity in maize rhizosphere soil, and no significant differences were observed between monocultured and intercropped maize. The Shannon index of soybean rhizosphere soil was significantly higher in intercropping treatments than in monoculture treatments for the corresponding nitrogen levels. The AMF diversity in the roots of maize showed different trends to those in the soil. The dominant genera in the present study were Glomus&#95;f&#95;Glomeraceae, Paraglomus, and Gigaspora, which occupied 55.52%, 9.18%, and 8.20% of the rhizosphere soil and 65.35%, 5.32%, and 17.16% of the roots, respectively. Our study showed that the abundance of the dominant genus, Glomus&#95;f&#95;Glomeraceae in maize soil and roots significantly increased in intercropping treatments compared with monoculture treatments, and it also increased with the increase in nitrogen application levels. In soybean soil and roots, the abundance of Glomus&#95;f&#95;Glomeraceae decreased with the increase in nitrogen application levels. The results of the redundancy and correlations analyses indicated that the changes in the AMF diversity and community in intercropping areas were significantly associated with alterations of the soil total nitrogen and alkali-hydrolysable nitrogen due to the interactions between maize and soybeans in intercropping systems with different nitrogen fertilizer application rates., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Total synthesis of Myc-IV(C16:0, S) via automated electrochemical assembly has been accomplished. This tetrasaccharide has been studied as a symbiotic signal molecule of Arbuscular Mycorrhiza fungi. We have achieved stereoselective synthesis of a disaccharide building block using the mixed-electrolyte system for electrochemical glycosylation; 2 + 1+1 strategy enables us to access the tetrasaccharide precursor and complete the synthesis Myc-IV(C16:0, S) efficiently., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Using of arbuscular mycorrhizal fungi (AMF) has emerged as a new technique to alleviate the toxic metals stress through changing their chemical behavior. The present work was conducted as a factorial arrangement based on a completely randomized design to study the inoculation effects of Glomus intraradices, Glomus mosseae and Glomus etunicatum, on Pb and Zn fractions in the rhizosphere of alfalfa by using rhizobox technique in two agricultural soils with different Zn and Pb concentrations [with low (LH) and high (HH) concentration levels]. The results showed that AMF colonization promoted plant growth and lowered the shoot and root Pb and shoot Zn concentrations in the studied soils compared to uninoculated treatments. Mycorrhizal colonization significantly increased the Ca(NO 3 ) 2 - extractable Zn and ORG-Zn (respectively 500 and 59.6% more than the uninoculated treatment) and decreased the OXI-Zn (20.32% less than the none inoculated treatment) in the HH soil. By contrast, mycorrhizae slightly increased the CARB, OXI and ORG-Zn forms in the LH soil compared to the uninoculation condition. In the AMF- treated HH soil, an increase was recorded in the Ca(NO 3 ) 2 - extractable Pb, EXCH-Pb and CARB-Pb (respectively, 17.65, 3.09 and 14.22% compared to the none inoculated treatment) and a decrease in the OXI and ORG-Pb forms (respectively, 28.79 and 13.51% compared to the uninoculated treatment). A reverse status was observed for Pb changes in the LH soil. Depending on the contamination level, the mycorrhizal inoculation differentially affected the Pb and Zn fractions at different distances from the root surface. In the LH soil, at <5 mm distance (i.e. rhizospheric soil), the mycorrhizal inoculation decreased the CARB (about 17.99%) and OXI -Zn (about 29.63%) forms compared to bulk soil (i.e. > 5 mm distance) while ORG-Zn was increased up to 48.63%. However, Ca(NO 3 ) 2 - extractable, CARB and ORG-Pb was increased in rhizosphere soil (respectively, 89.33, 3.84 and 6.14%) and OXI-Pb was decreased up to 10.36% compared to the bulk soil. In the HH soil, mycorrhizal inoculation increased the CARB and OXI-Zn (respectively, 1.76 and 5.71%) and OXI-Pb fractions (11.56%) compared to the <5 mm distances. Whereas, it reduced the Ca(NO 3 ) 2 - extractable, EXCH, and ORG-Zn (Respectively, 52.70, 19.19 and 30.16%) and Ca(NO 3 ) 2 - extractable, CARB and ORG-Pb (respectively, 47.18, 3.70 and 5.79%). These results revealed that depending on the soil contamination level and nature of the element, AMF colonization affects biogeochemical fractions of the metals and their accumulation in the plant tissues., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Heavy metal (HM) pollution of soils is one of the most important and unsolved environmental problems affecting the world, with alternative solutions currently being investigated through different approaches. Arbuscular mycorrhizal fungi (AMF) are soil inhabitants that form symbiotic relationships with plants. This alleviates HM toxicity in the host plant, thereby enhancing tolerance. However, the few investigations that have addressed the presence of metals in the fungus structures were performed under experimental conditions, with there being no results reported for Pb. The current study represents a first approximation concerning the capability of spores to accumulate Pb in the AMF community present in a Pb polluted soil under field conditions. Micro X-ray fluorescence was utilized to obtain a direct observation of Pb in spores, and the innovation of total reflection X-ray fluorescence was applied to obtain Pb quantification in spores. The AMF community included species of Ambisporaceae, Archaeosporaceae, Gigasporacea, Glomeraceae and Paraglomeraceae, and was tolerant to high Pb concentrations in soil. Pb accumulation in AMF spores was demonstrated at the community level and corroborated by direct observation of the most abundant spores, which belonged to the Gigasporaceae group. Spore Pb accumulation is possibly dependent on the AMF and host plant species., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Interactions of macrofungi with U, Th, Pb and Ag were investigated in the former ore mining district of Příbram, Czech Republic. Samples of saprotrophic (34 samples, 24 species) and ectomycorrhizal (38 samples, 26 species) macrofungi were collected from a U-polluted Norway spruce plantation and tailings and analyzed for metal content. In contrast to Ag, which was highly accumulated in fruit-bodies, concentrations of U generally did not exceed 3mg/kg which indicates a very low uptake rate and efficient exclusion of U from macrofungi. In ectomycorrhizal tips (mostly determined to species level by DNA sequencing), U contents were practically identical with those of the non-mycorrhizal fine spruce roots. These findings suggest a very limited role of macrofungi in uptake and biotransformation of U in polluted forest soils. Furthermore, accumulation of U, Th, Pb and Ag in macrofungal fruit-bodies apparently does not depend on total content and chemical fractionation of these metals in soils (tested by the BCR sequential extraction in this study)., (Copyright © 2014 Elsevier B.V. All rights reserved.)

A water soluble glucan (PS-I) was isolated from the hot aqueous extract of the fruit bodies of an ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. The total hydrolysis, methylation analysis, periodate oxidation, and NMR ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC) studies revealed the presence of the following repeating unit in the polysaccharide: This glucan showed excellent activation of macrophages as well as splenocytes and thymocytes in vitro., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

One method of mobilization of iron by mycorrhizal organisms is through the secretion of small organic chelators called siderophores. Hydroxamate donor chelators are a common type of siderophore that is frequently used by fungal organisms. The primary siderophore that is produced by fungi from the genera Ceratobasidium and Rhizoctonia is the tripodal trishydroxamate siderophore basidiochrome. To gain some insight into the iron uptake mechanisms of these symbiotic fungi, the iron binding characteristics of basidiochrome were determined. It was found that basidiochrome exhibits a log β(110) of 27.8±0.1 and a pFe value of 25.0. These values are similar to those of another fungal trishydroxamate siderophore, ferrichrome. The similarity in iron affinity between the two siderophores suggests that the structure of the backbone has little influence in complex formation due to the length of the pendant arms, although the identity of the terminating groups of the pendant arms is likely related to complex stability. The role of basidiochrome in the biogeochemical cycling of iron is also discussed., (Copyright © 2011 Elsevier Inc. All rights reserved.)

Organophosphorus pesticides in crops and soil pose a serious threat to public health and environment. Arbuscular mycorrhizal (AM) fungi may make a contribution to organophosphate degradation in soil and consequently decrease chemical residues in crops. A pot culture experiment was conducted to investigate the influences of Glomus caledonium 90036 and Acaulospora mellea ZZ on the dynamics of phoxim residues in green onion (Allium fistulosum L.) and soil at different harvest dates after phoxim application. Results show that mycorrhizal colonization rates of inoculated plants were higher than 70%. Shoot and root fresh weights did not vary with harvest dates but increased significantly in AM treatments. Phoxim residues in plants and soil decreased gradually with harvest dates, and markedly reduced in AM treatments. Kinetic analysis indicated that phoxim degradation in soil followed a first-order kinetic model. AM inoculation accelerated the degradation process and reduced the half-life. G. caledonium 90036 generally produced more pronounced effects than A. mellea ZZ on both the plant growth and phoxim residues in plants and soil. Our results indicate a promising potential of AM fungi for the control of organophosphate residues in vegetables, as well as for the phytoremediation of organophosphorus pesticide-contaminated soil., (Copyright © 2010 Elsevier B.V. All rights reserved.)

Camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. In recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. In this study we have isolated endophytic fungi strains from Apodytes dimidiata (Icacinaceae), a medium sized tree from the Western Ghats, India. The fungi were identified as Fusarium solani using both ITS rDNA sequencing and spore morphology. Two strains, MTCC 9667 and MTCC 9668 were isolated, both of which produced camptothecin and 9-methoxycamptothecin in their mycelia; one of the strains, MTCC 9668 also produced 10-hydroxycamptothecin, though in small amounts. The yields of camptothecin in MTCC 9667 and MTCC 9668 were 37 and 53 microg/100g, respectively, after 4 days of incubation in broth culture. The yields of 10-hydroxycamptothecin and 9-methoxycamptothecin in MTCC 9668 were 8.2 and 44.9 microg/100g, respectively. Further research in optimizing the culture conditions of these fungal strains might permit their application for the production of camptothecin and 10-hydroxycamptothecin., (2009. Published by Elsevier Ltd. All rights reserved.)

A field study of the natural attenuation occurring in a slag heap contaminated with high available cadmium was carried out. The aims of this research were: to determine plants colonizing this slag heap; to analyze colonization and morphological biodiversity of spores of arbuscular mycorrhizal fungi (AMF); to determine spore distribution in undisturbed samples; to know mycelium and glomalin abundance in the rhizosphere of these plants, and to investigate glomalin participation in Cd-stabilization. Forming vegetal islands, 22 different pioneering plant species from 11 families were colonizing the slag heap. The most common plants were species of Fabaceae, Asteraceae and Poaceae. Almost all plants were hosting AMF in their roots, and spores belonging to Gigaspora, Glomus, Scutellospora and Acaulospora species were observed. Micromorphological analysis showed that spores were related to decomposing vegetal residues and excrements, which means that mesofauna is contributing to their dispersion in the groundmass. Mycelium mass ranged from 0.11 to 26.3 mg/g, which contained between 13 and 75 mg of glomalin/g. Slag-extracted total glomalin was between 0.36 and 4.74 mg/g. Cadmium sequestered by glomalin extracted from either slag or mycelium was 0.028 mg/g. The ecological implication of these results is that organisms occupying vegetal patches are modifying mine residues, which contribute to soil formation.

Polyphosphate is ubiquitous among living organisms and has a variety of biochemical functions. Arbuscular mycorrhizal fungi have been known to accumulate polyphosphate as a key compound for their function. However, an enzymatic assay using polyphosphate kinase (PPK) reverse reaction, in which polyphosphate is converted to adenosine triphosphate (ATP) and quantified by luciferase assay, failed to detect accumulation of polyphosphate in some mycorrhizal root. When yeast exopolyphosphatase (PPX) was applied to these samples, a much higher polyphosphate level was detected than when the PPK assay was applied. Detailed analysis of substrate chain length specificity of these methods using polyphosphate chain length standards revealed that the PPX method was the most appropriate to detect short-chain polyphosphate. The average chain length of the shortest polyphosphate fraction that could be quantified with more than 50% efficiency was 3 for the PPX method and 38 for the PPK method. It was also suggested that the ratio of the PPK value to the PPX value may be useful as a simple and relative index to compare polyphosphate chain length distribution in different samples.

The amount of glomalin-related soil protein (GRSP), a glycoprotein produced by arbuscular mycorrhizal fungi (AMF), its contribution to the sequestering of Cu and Zn in the soil, and the microsite variation of other soil traits (pH, water-stable aggregates--[WSA], soil organic carbon--[SOC]) was studied in a semi-arid Mediterranean ecosystem near a copper smelter and affected by deposit of metal-rich particles since 1964. Rhizospheric (R) and non-rhizospheric (NR) soil of four representative plants (Argemone subfusiformis, Baccharis linearis, Oenothera affinis and Polypogon viridis) was analyzed. The results showed a strong variability in GRSP (6.6-36.8 mg g(-1)), Cu content (62-831 mg kg(-1) for the total Cu and 5.8-326 mg kg(-1) for the available Cu) and pH (4.2-5.5) in the different plant and rhizospheric zones analyzed. A strong relationship between the GRSP with the soil Cu and Zn contents was found (r=0.89 and 0.76 for Cu and Zn respectively, p<0.001). The GRSP-bound Cu ranged from 3.76 to 89.0 mg g(-1) soil and represents 1.44-27.5% of the total Cu content in soil. Moreover, the WSA reached 89% in P. viridis R. For this plant, the C contained in GRSP represented up to 89% of SOC, and this coincided with the most extreme conditions of soil degradation within the ecosystem (the highest content of heavy metals and low pH values). This study provides evidence on the role of the GRSP in Cu and Zn sequestration and suggests a highly efficient mechanism of AMF to mitigate stress leading to stabilization of soils highly polluted by mining activities.

Colonization of the roots of leek (Allium porrum L.) by the arbuscular mycorrhizal fungus Glomus intraradices induced the formation of apocarotenoids, whose accumulation has been studied over a period of 25 weeks. Whereas the increase in the levels of the dominating cyclohexenone derivatives resembles the enhancement of root length colonization, the content of mycorradicin derivatives remains relatively low throughout. Structural analysis of the cyclohexenone derivatives by mass spectrometry and NMR spectroscopy showed that they are mono- and diglycosides of 13-hydroxyblumenol C and blumenol C acylated with 3-hydroxy-3-methyl-glutaric and/or malonic acid. Along with the isolation of three known compounds five others are shown to be hitherto unknown members of the fast-growing family of mycorrhiza-induced cyclohexenone conjugates.

The distribution of lead and zinc in glomalin-related soil protein (GRSP), a widespread glycoprotein presumably produced by arbuscular mycorrhizal fungi (AMF) in soil, and in some other soil fractions (soil organic matter - [SOM], carbonates, phosphates, etc.) was studied in soils from an area near a lead smelter that differed in SOM, carbonates and heavy metal (HM) content. Total GRSP represented 5.4-21.2% of the SOM and was positively correlated with the soil Pb and Zn concentrations (r=0.57 and 0.66, p=0.007 and p=0.001 for Pb and Zn, respectively). Pb and Zn were predominantly bound to carbonates and organic matter. The amount of lead bound to GRSP varied between 0.69 and 23.4 mg g(-1) DW GRSP which is 0.8-15.5% of the total soil Pb. The amount of GRSP-bound metal was positively correlated with the total concentration in the case of Pb (r=0.90, p=0.000) but the opposite was found for Zn (r=-0.41, p=0.048), indicating that GRSP predominantly binds Pb. The percentages of HM-GRSP in HM-SOM were variable and were not correlated with SOM content.

Metabolite profiling of soluble primary and secondary metabolites, as well as cell wall-bound phenolic compounds from roots of barrel medic (Medicago truncatula) was carried out by GC-MS, HPLC and LC-MS. These analyses revealed a number of metabolic characteristics over 56 days of symbiotic interaction with the arbuscular mycorrhizal (AM) fungus Glomus intraradices, when compared to the controls, i.e. nonmycorrhizal roots supplied with low and high amounts of phosphate. During the most active stages of overall root mycorrhization, elevated levels of certain amino acids (Glu, Asp, Asn) were observed accompanied by increases in amounts of some fatty acids (palmitic and oleic acids), indicating a mycorrhiza-specific activation of plastidial metabolism. In addition, some accumulating fungus-specific fatty acids (palmitvaccenic and vaccenic acids) were assigned that may be used as markers of fungal root colonization. Stimulation of the biosynthesis of some constitutive isoflavonoids (daidzein, ononin and malonylononin) occurred, however, only at late stages of root mycorrhization. Increase of the levels of saponins correlated AM-independently with plant growth. Only in AM roots was the accumulation of apocarotenoids (cyclohexenone and mycorradicin derivatives) observed. The structures of the unknown cyclohexenone derivatives were identified by spectroscopic methods as glucosides of blumenol C and 13-hydroxyblumenol C and their corresponding malonyl conjugates. During mycorrhization, the levels of typical cell wall-bound phenolics (e.g. 4-hydroxybenzaldehyde, vanillin, ferulic acid) did not change; however, high amounts of cell wall-bound tyrosol were exclusively detected in AM roots. Principal component analyses of nonpolar primary and secondary metabolites clearly separated AM roots from those of the controls, which was confirmed by an hierarchical cluster analysis. Circular networks of primary nonpolar metabolites showed stronger and more frequent correlations between metabolites in the mycorrhizal roots. The same trend, but to a lesser extent, was observed in nonmycorrhizal roots supplied with high amounts of phosphate. These results indicate a tighter control of primary metabolism in AM roots compared to control plants. Network correlation analyses revealed distinct clusters of amino acids and sugars/aliphatic acids with strong metabolic correlations among one another in all plants analyzed; however, mycorrhizal symbiosis reduced the cluster separation and enlarged the sugar cluster size. The amino acid clusters represent groups of metabolites with strong correlations among one another (cliques) that are differently composed in mycorrhizal and nonmycorrhizal roots. In conclusion, the present work shows for the first time that there are clear differences in development- and symbiosis-dependent primary and secondary metabolism of M. truncatula roots.

Almost all land plants have developed a symbiosis with arbuscular mycorrhizal fungi. Establishment of the association is accompanied by structural changes in the plant root. During arbuscule formation fungal hyphae penetrate the root apoplast and install highly specialized interfaces for solute transport between plant and fungus. The periarbuscular membrane which is part of the plant plasma membrane surrounding arbuscular structures was shown to harbour a high density of different transport systems. Among these also expression of aquaporins was described, which potentially can act as a low affinity transport system for ammonia or ammonium. The present study provides data for expression, localization and function of plant aquaporins in the periarbuscular membrane of mycorrhizal Medicago truncatula plants.

Colonization of roots of Ornithogalum umbellatum by the arbuscular mycorrhizal fungus Glomus intraradices induced the accumulation of different types of apocarotenoids. In addition to the mycorrhiza-specific occurrence of cyclohexenone derivatives and the "yellow pigment" described earlier, free mycorradicin and numerous mycorradicin derivatives were detected in a complex apocarotenoid mixture for the first time. From the accumulation pattern of the mycorradicin derivatives their possible integration into the continuously accumulating "yellow pigment" is suggested. Structure analyses of the cyclohexenone derivatives by MS and NMR revealed that they are mono-, di- and branched triglycosides of blumenol C, 13-hydroxyblumenol C, and 13-nor-5-carboxy-blumenol C, some of which contain terminal rhamnose as sugar moiety.