Search

Genetic diversity of Moniezia spp. from domestic ruminants in Senegal and Ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as Moniezia benedeni. On the other hand, the tapeworms obtained from sheep and goat were identified as Moniezia expansa or could not be identified because of the lack of diagnostic morphologic character, i.e. interproglottidal glands (IPGs). Phylogenetic analysis based on cox1 gene sequences revealed that the worms from sheep/goat and cattle formed distinct clades, and three mitochondrial lineages were confirmed within the sheep/goat tapeworms. The maximum pairwise divergences among the three mitochondrial linages were about 3% in cox1 and 0.1% in SSU rDNA, while that between the worms from sheep/goat and cattle reached 13% in cox1 and 2.7% in SSU rDNA. All of the three mitochondrial lineages contained tapeworms morphologically identified as M. expansa, and the tapeworms without IPGs were confirmed in one of the three lineages, indicating the tapeworms without IPGs were also M. expansa., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)

To identify the etiologic agents of cystic echinococcosis in Ethiopia, unilocular hydatid cysts were collected from 11 sheep, 16 cattle and 16 camels slaughtered in abattoirs of Aweday, Jijiga, Haramaya and Addis Ababa during June 2010 to February 2011. A PCR-based DNA sequencing of the mitochondrial cytochrome oxidase c subunit 1 gene (cox1) was conducted for 40 cysts. The majority of cysts (87.5%) were identified as Echinococcus granulosus sensu stricto and the rest as Echinococcus canadensis. The fertile cysts of E. granulosus s.s. were found only from sheep, although it occurred in all the host species. The predominance of E. granulosus s.s. has important implications for public health since this species is the most typical causative agent of human cystic echinococcosis worldwide. The major cox1 haplotype of E. granulosus s.s. detected in Ethiopia was the same as that has been reported to be most common in Peru and China. However, a few cox1 haplotypes unique to Ethiopia were found in both of the two Echinococcus species. The present regional data would serve as baseline information in determining the local transmission patterns and in designing appropriate control strategies., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)