1. All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse
- Author
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Hayley A. Bounds, Masato Sadahiro, William D. Hendricks, Marta Gajowa, Karthika Gopakumar, Daniel Quintana, Bosiljka Tasic, Tanya L. Daigle, Hongkui Zeng, Ian Antón Oldenburg, and Hillel Adesnik
- Subjects
CP: Neuroscience ,Biology (General) ,QH301-705.5 - Abstract
Summary: Determining which features of the neural code drive behavior requires the ability to simultaneously read out and write in neural activity patterns with high precision across many neurons. All-optical systems that combine two-photon calcium imaging and targeted photostimulation enable the activation of specific, functionally defined groups of neurons. However, these techniques are unable to test how patterns of activity across a population contribute to computation because of an inability to both read and write cell-specific firing rates. To overcome this challenge, we make two advances: first, we introduce a genetic line of mice for Cre-dependent co-expression of a calcium indicator and a potent soma-targeted microbial opsin. Second, using this line, we develop a method for read-out and write-in of precise population vectors of neural activity by calibrating the photostimulation to each cell. These advances offer a powerful and convenient platform for investigating the neural codes of computation and behavior.
- Published
- 2023
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