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Your search keyword '"Marino, Giovanna"' showing total 3 results
Start Over Author "Marino, Giovanna" Publisher elsevier
3 results on '"Marino, Giovanna"'

1. The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system

Author

Sammouth, Sophie, Roque D'Orbcastel, Emmanuelle, Gasset, Eric, Lemarie, Gilles, Breuil, Gilles, Marino, Giovanna, Coeurdacier, Jean-luc, Fivelstad, Sveinung, Blancheton, Jean-paul, Sammouth, Sophie, Roque D'Orbcastel, Emmanuelle, Gasset, Eric, Lemarie, Gilles, Breuil, Gilles, Marino, Giovanna, Coeurdacier, Jean-luc, Fivelstad, Sveinung, and Blancheton, Jean-paul

Abstract

Sea bass (Dicentrarchus tabrax) (135 +/- 4 g) were reared under tank-based recirculating aquaculture system for a 63-day period at four densities: 10, 40, 70, 100 kg m(-3). Fish performance, stress indicators (plasma cortisol, proteonemia plus other blood parameters-Na+, K+, glucose, pH, total CO2- and water quality were monitored. At the end of the 63-day period, resistance to infection was also studied by a nodavirus challenge. A 25-day test was performed on fish from two extreme densities (10 and 100 kg m(3)) and one intermediate density (40 kg m(3)). With regards to the different density treatments, there was no significant difference between the daily feed intake (DFI) and the specific growth rate (SGR) up to a density of 70 kg m(-3). No significant difference was found between treatments concerning the feed conversion ratio (FCR) and the mortality rate. No density effect was observed on the fish stress level (plasma cortisol) or on sensitivity to the nodavirus challenge. Under these experimental rearing conditions, the density above 70 kg m(-3) has an impact on growth performance (DFI and SGR) indicators and also some blood parameters (CO2) at the highest density tested (100 kg m(-3)). This study suggests that a density up to 70 kg m(-3) has no influence on sea bass performance and welfare. At 100 kg m(-3), average specific growth rate was decreased by 14% without welfare deterioration according to the welfare indicators monitored. (C) 2008 Elsevier B.V. All rights reserved.

Published
2009
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2. Environmental hazard of yperite released at sea: sublethal toxic effects on fish.

Author

Della Torre C, Petochi T, Farchi C, Corsi I, Dinardo MM, Sammarini V, Alcaro L, Mechelli L, Focardi S, Tursi A, Marino G, and Amato E

Subjects
Animals, Cytochrome P-450 CYP1A1 metabolism, Fish Proteins metabolism, Glucuronosyltransferase metabolism, Glutathione Transferase metabolism, Micronucleus Tests, Muscle, Skeletal drug effects, Muscle, Skeletal pathology, Oceans and Seas, Skin drug effects, Skin pathology, Anguilla abnormalities, Anguilla genetics, Anguilla metabolism, Mustard Gas toxicity, Water Pollutants, Chemical toxicity
Abstract

The aim of this study was to evaluate the potential toxicological effects on fish related to the leakage of yperite from rusted bomb shells dumped at sea. Both in vivo and field studies have been performed. As for the in vivo experiment, specimen of European eel were subcutaneously injected with 0.015, 0.15 and 1.5mg/kg of yperite and sacrificed after 24 and 48 h. In the field study, specimen of Conger eel were collected from a dumping site in the Southern Adriatic Sea. The presence/absence of yperite in tissues, genotoxicity, detoxification enzymes, histological alterations and gross abnormalities were investigated. Results of the in vivo experiment showed a significant increase of EROD activity at both 24h and 48 h. UGT activity increased significantly at 48 h post injection. An acute inflammatory response after 24h in skin layers and muscle was observed, associated to cell degeneration and necrosis after 48 h at the highest dose. On field, comet assay revealed genotoxicity in gills of fish from the dumping site. Specimen from the dumping site showed significantly higher EROD activities compared to controls, deep ulcers and papules on skin together with liver and spleen histopathological lesions., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Published
2013
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3. DNA damage, severe organ lesions and high muscle levels of As and Hg in two benthic fish species from a chemical warfare agent dumping site in the Mediterranean Sea.

Author

Della Torre C, Petochi T, Corsi I, Dinardo MM, Baroni D, Alcaro L, Focardi S, Tursi A, Marino G, Frigeri A, and Amato E

Subjects
Animals, Arsenicals analysis, Arsenicals metabolism, Chemical Warfare Agents analysis, Chemical Warfare Agents metabolism, Chromatography, Gas, Comet Assay, Cytochrome P-450 CYP1A1 metabolism, DNA Damage, Food Contamination analysis, Gills drug effects, Gills pathology, Glucuronosyltransferase metabolism, Industrial Waste analysis, Liver chemistry, Liver drug effects, Liver metabolism, Mediterranean Sea, Mercury Compounds analysis, Mercury Compounds metabolism, Muscle, Skeletal chemistry, Muscle, Skeletal drug effects, Muscle, Skeletal metabolism, Refuse Disposal, Seafood analysis, Skin drug effects, Skin pathology, Spleen drug effects, Spleen pathology, Water Pollutants, Chemical analysis, Water Pollutants, Chemical metabolism, Chemical Warfare Agents toxicity, Eels physiology, Fishes physiology, Industrial Waste adverse effects, Water Pollutants, Chemical toxicity
Abstract

The aim of the present study was to evaluate the environmental threat to benthic species from chemical weapons dumped in the southern Adriatic Sea. An ecotoxicological approach using chemical analysis and biological responses was applied, in two sentinel species: the Blackbelly rosefish Helicolenus dactylopterus and European conger Conger conger. Specimen were collected in a stretch of sea, where had been dumped war materials and from a reference site free of ordnance. Residues of yperite, Hg and As were measured in fish fillets. Skin, liver, kidney and spleen were examined for histopathological and macroscopical lesions. Liver detoxifying capacities (EROD and UDPGT) and genotoxicity (comet assay) were also investigated. As and Hg levels were three-four times higher than those from the reference site in both species (p<0.001). Both species captured in dumping site showed clear signs of chronic illness according to the health assessment index (HAI). Deep ulcers and nodules were observed on skin and external organs. Histological lesions such as periportal and bile duct fibrosis, pericholangitis, steatosis, granuloma and elevated splenic MMCs were detected in liver and spleen. Significantly higher EROD activities were also found in both species from dumping site (p<0.01). Comet assay revealed genotoxicty in gills of C. conger from dumping site, indicating uptake of chemical warfare agents through fish gills. European conger was found to be a more sensitive bioindicator of this type of contamination than the Blackbelly rosefish., ((c) 2010 Elsevier B.V. All rights reserved.)

Published
2010
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