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Your search keyword '"Maria Chountoulesi"' showing total 3 results
Start Over Author "Maria Chountoulesi" Publisher elsevier
3 results on '"Maria Chountoulesi"'

1. One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Author

Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Kety Giannetti, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Efthymios Kyrodimos, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios Valakos, Giannis Vatsellas, Russell Petty, Dimitris Thanos, Marco Demaria, Konstantinos Evangelou, Raffaella Di Micco, and Vassilis G. Gorgoulis

Subjects
Cell Biology, Cell isolation, Cell-based Assays, Flow Cytometry, Cancer, Molecular/Chemical Probes, Science (General), Q1-390
Abstract

Summary: Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present.For complete details on the use and execution of this protocol, please refer to Magkouta et al.1

Published
2024
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2. List of contributors

Author

Katerina Anagnostou, Maria Chountoulesi, Francesco Cilurzo, Costas Demetzos, Patricia Diaz-Rodriguez, Silvia Franzè, Jaspreet Singh Gulati, David Henson, Mario Jug, Indu Pal Kaur, Mariana Landin, Sotiris Michaleas, Umberto M. Musazzi, David Nardo, Nikolaos Naziris, Dorota Neugebauer, Natassa Pippa, Stergios Pispas, Mohhammad Ramzan, Carmen Remuñán-López, Helena Rouco, Simarjot Kaur Sandhu, Garima Sharma, Angeliki Siamidi, Joga Singh, Mandeep Singh, Athanasios Skouras, Joe E. Springer, Minas Stylianakis, Vincent J. Venditto, and Marilena Vlachou

Published
2020

3. Stimuli-responsive nanocarriers for drug delivery

Author

Natassa Pippa, Maria Chountoulesi, Nikolaos Naziris, Stergios Pispas, and Costas Demetzos

Subjects
Stimuli responsive, Chemistry, Liquid crystalline, Drug delivery, Nanotechnology, Nanocarriers
Abstract

Stimuli-responsive drug delivery nanosystems have received great attention recently, due to their significant advantages, such as their ability for targeted content release, by responding to the altered conditions of the pathological tissues, such as temperature and pH. Toward the development of new and more effective stimuli-responsive nanosystems for biomedical applications, a great variety of different smart stimuli-responsive polymers has been exploited. The combination of different in nature biomaterials, such as lipids and stimuli-responsive polymers, can provide chimeric/mixed advanced drug delivery liposomal nanosystems and also stabilize other nonlamellar lyotropic lipidic liquid crystalline nanosystems with upgraded functionality. In this chapter, the two abovementioned categories of stimuli-responsive lipidic nanocarriers are examined through different literature examples, also highlighting useful characterization techniques that should be applied toward the rational development and evaluation of this highly promising class of nanosystems.

Published
2020

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