1. Expression analysis of microRNAs and mRNAs in myofibroblast differentiation of lung resident mesenchymal stem cells.
- Author
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Wang C, Cao H, Gu S, Shi C, Chen X, and Han X
- Subjects
- Animals, Cell Differentiation genetics, Gene Expression Regulation, Developmental genetics, Humans, Idiopathic Pulmonary Fibrosis genetics, Idiopathic Pulmonary Fibrosis metabolism, Idiopathic Pulmonary Fibrosis pathology, Kruppel-Like Factor 4, Lung cytology, Lung metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Myofibroblasts cytology, Myofibroblasts metabolism, Transforming Growth Factor beta1 genetics, Kruppel-Like Transcription Factors genetics, Lung growth & development, MicroRNAs genetics, Transcription Factors genetics, Transforming Growth Factor beta1 metabolism, Tumor Suppressor Proteins genetics
- Abstract
Idiopathic pulmonary fibrosis (IPF) is a serious lung disease that involved the myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSCs). However, the specific molecular mechanisms of myofibroblast differentiation of LR-MSCs still remain a mystery. In this study, a comprehensive analysis of miRNAs and mRNAs changes in LR-MSCs treated with TGF-β1 was performed. Through computational approaches, the pivotal roles of differentially expressed miRNAs that were associated with tight junction, pathways in cancer, focal adhesion, and cytokine-cytokine receptor interaction were shown. Kruppel-like factor 4 (Klf4) and inhibitor of growth family, member 5 (Ing5) may be the targets for the therapy of pulmonary fibrosis by inhibiting myofibroblast differentiation of LR-MSCs and EMT. Collectively, a molecular paradigm for understanding myofibroblast differentiation of LR-MSCs in IPF was provided by the integrated miRNA/mRNA analyses., Competing Interests: Declaration of interest statement The authors declare no competing interests., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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